The SMC-5/6 Complex and the HIM-6 (BLM) Helicase Synergistically Promote Meiotic Recombination Intermediate Processing and Chromosome Maturation during Caenorhabditis elegans Meiosis.
PLoS Genetics 12(3):e1005872 (2016)
Meiotic recombination is essential for the repair of programmed double strand breaks (DSBs) to generate crossovers (COs) during meiosis. The efficient processing of meiotic recombination intermediates not only needs various resolvases but also requires proper meiotic chromosome structure. The Sm...
Micro-proteomics with iterative data analysis: Proteome analysis in C. elegans at the single worm level.
Proteomics 16(3):381 (2016)
Proteomics studies typically analyze proteins at a population level, using extracts prepared from tens of thousands to millions of cells. The resulting measurements correspond to average values across the cell population and can mask considerable variation in protein expression and function betw...
Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA.
Cell Reports 13(11):2565 (2015)
We present the crystal structure of the junction-resolving enzyme GEN1 bound to DNA at 2.5 Å resolution. The structure of the GEN1 protein reveals it to have an elaborated FEN-XPG family fold that is modified for its role in four-way junction resolution. The functional unit in the crystal is a m...
Both Chromosome Decondensation and Condensation Are Dependent on DNA Replication in C. elegans Embryos.
Cell Reports 12(3):405 (2015)
During cell division, chromatin alternates between a condensed state to facilitate chromosome segregation and a decondensed form when DNA replicates. In most tissues, S phase and mitosis are separated by defined G1 and G2 gap phases, but early embryogenesis involves rapid oscillations between re...
Global Proteomics Analysis of the Response to Starvation in C. elegans.
Molecular & Cellular Proteomics 14(7):1989 (2015)
Periodic starvation of animals induces large shifts in metabolism but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. ...
Combined hereditary and somatic mutations of replication error repair genes result in rapid onset of ultra-hypermutated cancers.
Nature Genetics 47(3):257 (2015)
DNA replication-associated mutations are repaired by two components: polymerase proofreading and mismatch repair. The mutation consequences of disruption to both repair components in humans are not well studied. We sequenced cancer genomes from children with inherited biallelic mismatch repair d...
Erratum: Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans.
Nature Communications 6:6352 (2015)
GEN1 from a thermophilic fungus is functionally closely similar to non-eukaryotic junction-resolving enzymes.
Journal of Molecular Biology 426(24):3946 (2014)
Processing of Holliday junctions is essential in recombination. We have identified the gene for the junction-resolving enzyme GEN1 from the thermophilic fungus Chaetomium thermophilum and expressed the N-terminal 487-amino-acid section. The protein is a nuclease that is highly selective for four...
Tetraspanin (TSP-17) protects dopaminergic neurons against 6-OHDA-induced neurodegeneration in C. elegans.
PLoS Genetics 10(12):e1004767 (2014)
Parkinson's disease (PD), the second most prevalent neurodegenerative disease after Alzheimer's disease, is linked to the gradual loss of dopaminergic neurons in the substantia nigra. Disease loci causing hereditary forms of PD are known, but most cases are attributable to a combination of genet...
Having a direct look: analysis of DNA damage and repair mechanisms by next generation sequencing.
Experimental Cell Research 329(1):35 (2014)
Genetic information is under constant attack from endogenous and exogenous sources, and the use of model organisms has provided important frameworks to understand how genome stability is maintained and how various DNA lesions are repaired. The advance of high throughput next generation sequencin...
C. elegans whole-genome sequencing reveals mutational signatures related to carcinogens and DNA repair deficiency.
Genome Research 24(10):1624 (2014)
Mutation is associated with developmental and hereditary disorders, aging, and cancer. While we understand some mutational processes operative in human disease, most remain mysterious. We used Caenorhabditis elegans whole-genome sequencing to model mutational signatures, analyzing 183 worm popul...
Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans.
Nature Communications 5:5485 (2014)
The small ubiquitin-like modifier (SUMO), initially characterized as a suppressor of a mutation in the gene encoding the centromeric protein MIF2, is involved in many aspects of cell cycle regulation. The dynamics of conjugation and deconjugation and the role of SUMO during the cell cycle remain...
Stress response: anything that doesn't kill you makes you stronger.
Current Biology 23(22):R1012 (2013)
A new study shows that DNA damage not only elicits response pathways directly related to DNA repair but also induces a response that extensively overlaps with the pathogen infection pathway and confers resistance to both oxidative stress and heat shock.
Copyright © 2013 Elsevier Ltd. All rights...
RNA-binding protein GLD-1/quaking genetically interacts with the mir-35 and the let-7 miRNA pathways in Caenorhabditis elegans.
Open Biology 3(11):130151 (2013)
Messenger RNA translation is regulated by RNA-binding proteins and small non-coding RNAs called microRNAs. Even though we know the majority of RNA-binding proteins and microRNAs that regulate messenger RNA expression, evidence of interactions between the two remain elusive. The role of the RNA-b...
The NM23-H1/H2 homolog NDK-1 is required for full activation of Ras signaling in C. elegans.
Development 140(16):3486 (2013)
The group I members of the Nm23 (non-metastatic) gene family encode nucleoside diphosphate kinases (NDPKs) that have been implicated in the regulation of cell migration, proliferation and differentiation. Despite their developmental and medical significance, the molecular functions of these NDPK...
If you don't want them shed them.
Cell Research 23(2):171 (2013)
Seminal studies in C. elegans contributed to our general understanding of programmed cell death conferred by apoptosis. A recent study unravelled a new form of cell death in the worm and provided insights into its regulation. Affected cells are shed from intact tissues, a modality of death likel...
Combinatorial regulation of meiotic holliday junction resolution in C. elegans by HIM-6 (BLM) helicase, SLX-4, and the SLX-1, MUS-81 and XPF-1 nucleases.
PLoS Genetics 9(7):e1003591 (2013)
Holliday junctions (HJs) are cruciform DNA structures that are created during recombination events. It is a matter of considerable importance to determine the resolvase(s) that promote resolution of these structures. We previously reported that C. elegans GEN-1 is a symmetrically cleaving HJ res...
Germ cell apoptosis and DNA damage responses.
Advances in Experimental Medicine and Biology 757:249 (2013)
In the past 12 years, since the first description of C. elegans germ cell apoptosis, this area of research rapidly expanded. It became evident that multiple genetic pathways lead to the apoptotic demise of germ cells. We are only beginning to understand how these pathways that all require the CE...
LAAT-1 is the lysosomal lysine/arginine transporter that maintains amino acid homeostasis.
Science 337(6092):351 (2012)
Defective catabolite export from lysosomes results in lysosomal storage diseases in humans. Mutations in the cystine transporter gene CTNS cause cystinosis, but other lysosomal amino acid transporters are poorly characterized at the molecular level. Here, we identified the Caenorhabditis elegans...
The dynamics of replication licensing in live Caenorhabditis elegans embryos.
Journal of Cell Biology 196(2):233 (2012)
Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of Caenorhabditis elegans embryos. As expected, MC...