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  1. Effect of ionic liquids on the structure, stability and activity of two related α-amylases.

    International Journal of Biological Macromolecules 48(1):93 (2011) PMID 20946913

    We have investigated the influence of two water miscible ionic liquids on the activity, stability and structure of two related α-amylases from Bacillus amyloliquefaciens and Bacillus lichiniformis. Upon changes in ionic liquids concentrations, both enzymes activity and stability were reduced. Associ...
  2. Studies on the refolding process of recombinant horseradish peroxidase.

    Molecular Biotechnology 54(2):484 (2013) PMID 22872497

    Horseradish peroxidase (HRP) is an important heme-containing glyco-enzyme that has been used in many biotechnological fields. Valuable proteins like HRP can be obtained in sufficient amounts using Escherichia coli as an expression system. However, frequently, the expression of recombinant enzyme res...
  3. Purification and characterization of a thermostable phytate resistant alpha-amylase from Geobacillus sp. LH8.

    International Journal of Biological Macromolecules 46(1):27 (2010) PMID 19874846

    A thermophilic and amylolytic bacterium (LH8) was isolated from the hot spring of Larijan in Iran at 65 degrees C. Identification of strain LH8 by 16S rDNA sequence analysis showed that LH8 strain belongs to the Geobacillus sp. with 99% sequence similarity with the 16S rDNA of Geobacillus thermodeni...
  4. Extra EF Hand Unit (DX) Mediated Stabilization and Calcium Independency of α-Amylase.

    Molecular Biotechnology 53(3):270 (2013) PMID 22407721

    We used site-directed mutagenesis to replace histidine 58 with Asp (D), Ile (I), Tyr (Y), Phe (F), and Arg (R) at the seventh position of EF-hand-like motif. Upon the addition of an extra DX unit to the calcium-binding loop in H58D variant, thermal stability, catalytic activity, and chelating power...
  5. Molecular detection of human parechovirus type 1 in stool samples from children with diarrhea.

    International Journal of Infectious Diseases 16(9):e673 (2012) PMID 22771054

    We also compared this RT-PCR method with a cell culture procedure. Out of 472 samples, 112 (23.7%) were HPeV-1-positive. The incidence and prevalence of this virus was highest in children under 1 year old with diarrhea (p=0.036), and was higher in the spring and autumn than in summer and winter (p<0...
  6. Mesophilic alcohol dehydrogenase behavior in imidazolium based ionic liquids

    Journal of Molecular Liquids 161(3):139 (2011)

    We have investigated the influence of four water miscible ionic liquids on the activity, stability and structure of the mesophilic alcohol dehydrogenase from yeast. Upon changes in ionic liquids concentration, both activity and stability of the enzyme were affected. As the concentration of ionic liq...