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  1. Specificity enhancement towards phenolic substrate by immobilization of laccase on surface plasmon resonance sensor chip

    Journal of Molecular Catalysis B: Enzymatic 121:32 (2015)

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  2. A semi-rational approach to obtain an ionic liquid tolerant bacterial laccase through π-type interactions.

    International Journal of Biological Macromolecules 79:822 (2015) PMID 26054663

    Laccases are particularly promising enzymes for biotechnology and bioremediation purposes. They are among the most effective enzymes capable of catalyzing the degradation of phenolic compounds with poor water solubility. The technological utility of lacasses can be enhanced greatly by their use ...
  3. Phenol removal from refinery wastewater by mutant recombinant horseradish peroxidase.

    Biotechnology and Applied Biochemistry 61(2):226 (2014) PMID 24112382

    Application of mutated recombinant horseradish peroxidase (HRP) for phenol removal from refinery effluents is reported. Recombinant HRP produced in Escherichia coli suffers from the disadvantage of lacking glycosylation, which affects its catalytic efficiency and stability toward inactivating pa...
  4. Studies on the refolding process of recombinant horseradish peroxidase.

    Molecular Biotechnology 54(2):484 (2013) PMID 22872497

    Horseradish peroxidase (HRP) is an important heme-containing glyco-enzyme that has been used in many biotechnological fields. Valuable proteins like HRP can be obtained in sufficient amounts using Escherichia coli as an expression system. However, frequently, the expression of recombinant enzyme...
  5. Extra EF hand unit (DX) mediated stabilization and calcium independency of α-amylase.

    Molecular Biotechnology 53(3):270 (2013) PMID 22407721

    It is the common feature of α-amylases that calcium ion is required for their structural integrity and thermal stability. All amylases have at least one Ca(2+) per molecule; therefore amino acids involved in calcium binding are specific and conserved. In this study, sequence analysis revealed th...
  6. Molecular detection of human parechovirus type 1 in stool samples from children with diarrhea.

    International Journal of Infectious Diseases 16(9):e673 (2012) PMID 22771054

    Human parechovirus type 1 (HPeV-1) appears to be associated with gastrointestinal and respiratory infections, and occasionally with central nervous system symptoms. Since there is no accurate information about the incidence and epidemiology of HPeV-1 in Iran, this study was designed to describe ...
  7. Inhibition mediated stabilization effect of imidazolium based ionic liquids on alcohol dehydrogenase

    Journal of Molecular Liquids 170:66 (2012)

    Ionic liquids (ILs) have gained increasing attention as solvents in chemical and biotechnological applications. Since alcohol dehydrogenases are of prime interest in industrial field, the present study has been conducted to study the influence of imidazolium based ionic liquids on kine...
  8. Mesophilic alcohol dehydrogenase behavior in imidazolium based ionic liquids

    Journal of Molecular Liquids 161(3):139 (2011)

    The use of ionic liquids to replace organic solvents in biocatalytic processes has recently gained much attention. Despite the wide applications of oxidoreductases, there are few reports of their catalyzed reaction in ionic liquid. We have investigated the influence of four water miscible i...
  9. Effect of ionic liquids on the structure, stability and activity of two related α-amylases.

    International Journal of Biological Macromolecules 48(1):93 (2011) PMID 20946913

    Ionic liquids are recognized as green solvents for carbohydrates dissolution. However, only a limited number of studies have been carried out to investigate their effect on carbohydrate hydrolyzing enzymes. We have investigated the influence of two water miscible ionic liquids on the activity, s...
  10. Purification and characterization of a thermostable phytate resistant alpha-amylase from Geobacillus sp. LH8.

    International Journal of Biological Macromolecules 46(1):27 (2010) PMID 19874846

    A thermophilic and amylolytic bacterium (LH8) was isolated from the hot spring of Larijan in Iran at 65 degrees C. Identification of strain LH8 by 16S rDNA sequence analysis showed that LH8 strain belongs to the Geobacillus sp. with 99% sequence similarity with the 16S rDNA of Geobacillus thermo...