1. Leinamycin E1 acting as an anticancer prodrug activated by reactive oxygen species.

    PNAS 112(27):8278 (2015) PMID 26056295 PMCID PMC4500275

    Leinamycin (LNM) is a potent antitumor antibiotic produced by Streptomyces atroolivaceus S-140, featuring an unusual 1,3-dioxo-1,2-dithiolane moiety that is spiro-fused to a thiazole-containing 18-membered lactam ring. Upon reductive activation in the presence of cellular thiols, LNM exerts its ...
  2. Deciphering poxvirus gene expression by RNA sequencing and ribosome profiling.

    Journal of Virology 89(13):6874 (2015) PMID 25903347 PMCID PMC4468498

    The more than 200 closely spaced annotated open reading frames, extensive transcriptional read-through, and numerous unpredicted RNA start sites have made the analysis of vaccinia virus gene expression challenging. Genome-wide ribosome profiling provided an unprecedented assessment of poxvirus g...
  3. Quantitative profiling of initiating ribosomes in vivo.

    Nature Methods 12(2):147 (2015) PMID 25486063

    Cells have evolved exquisite mechanisms to fine-tune the rate of protein synthesis in response to stress. Systemic mapping of start-codon positions and precise measurement of the corresponding initiation rate would transform our understanding of translational control. Here we present quantitativ...
  4. Quantitative profiling of initiating ribosomes in vivo.

    Nature Methods 12(2):147 (2015) PMID 25486063

    Cells have evolved exquisite mechanisms to fine-tune the rate of protein synthesis in response to stress. Systemic mapping of start-codon positions and precise measurement of the corresponding initiation rate would transform our understanding of translational control. Here we present quantitativ...
  5. Quantitative profiling of initiating ribosomes in vivo.

    Nature Methods 12(2):147 (2015) PMID 25486063 PMCID PMC4344187

    Cells have evolved exquisite mechanisms to fine-tune the rate of protein synthesis in response to stress. Systemic mapping of start-codon positions and precise measurement of the corresponding initiation rate would transform our understanding of translational control. Here we present quantitativ...
  6. Quantitative profiling of initiating ribosomes in vivo.

    Nature Methods 12(2):147 (2015) PMID 25486063 PMCID PMC4344187

    Cells have evolved exquisite mechanisms to fine-tune the rate of protein synthesis in response to stress. Systemic mapping of start-codon positions and precise measurement of the corresponding initiation rate would transform our understanding of translational control. Here we present quantitativ...
  7. Quantitative profiling of initiating ribosomes in vivo.

    Nature Methods 12(2):147 (2015) PMID 25486063

    Cells have evolved exquisite mechanisms to fine-tune the rate of protein synthesis in response to stress. Systemic mapping of start-codon positions and precise measurement of the corresponding initiation rate would transform our understanding of translational control. Here we present quantitativ...
  8. Enediynes: Exploration of microbial genomics to discover new anticancer drug leads.

    Bioorganic & Medicinal Chemistry Letters 25(1):9 (2015) PMID 25434000

    The enediyne natural products have been explored for their phenomenal cytotoxicity. The development of enediynes into anticancer drugs has been successfully achieved through the utilization of polymer- and antibody-drug conjugates (ADCs) as drug delivery systems. An increasing inventory of enedi...
  9. Enediynes: Exploration of microbial genomics to discover new anticancer drug leads.

    Bioorganic & Medicinal Chemistry Letters 25(1):9 (2015) PMID 25434000 PMCID PMC4480864

    The enediyne natural products have been explored for their phenomenal cytotoxicity. The development of enediynes into anticancer drugs has been successfully achieved through the utilization of polymer- and antibody-drug conjugates (ADCs) as drug delivery systems. An increasing inventory of enedi...
  10. Comparative characterization of the lactimidomycin and iso-migrastatin biosynthetic machineries revealing unusual features for acyltransferase-less type I polyketide synthases and providing an opportunity to engineer new analogues.

    Biochemistry (Washington) 53(49):7854 (2014) PMID 25405956 PMCID PMC4270375

    Lactimidomycin (LTM, 1) and iso-migrastatin (iso-MGS, 2) belong to the glutarimide-containing polyketide family of natural products. We previously cloned and characterized the mgs biosynthetic gene cluster from Streptomyces platensis NRRL 18993. The iso-MGS biosynthetic machinery featured an acy...
  11. Comparative characterization of the lactimidomycin and iso-migrastatin biosynthetic machineries revealing unusual features for acyltransferase-less type I polyketide synthases and providing an opportunity to engineer new analogues.

    Biochemistry (Washington) 53(49):7854 (2014) PMID 25405956 PMCID PMC4270375

    Lactimidomycin (LTM, 1) and iso-migrastatin (iso-MGS, 2) belong to the glutarimide-containing polyketide family of natural products. We previously cloned and characterized the mgs biosynthetic gene cluster from Streptomyces platensis NRRL 18993. The iso-MGS biosynthetic machinery featured an acy...
  12. Comparative Characterization of the Lactimidomycin and iso-Migrastatin Biosynthetic Machineries Revealing Unusual Features for Acyltransferase-less Type I Polyketide Synthases and Providing an Opportunity To Engineer New Analogues.

    Biochemistry (Washington) 53(49):7854 (2014) PMID 25405956

    Lactimidomycin (LTM, 1) and iso-migrastatin (iso-MGS, 2) belong to the glutarimide-containing polyketide family of natural products. We previously cloned and characterized the mgs biosynthetic gene cluster from Streptomyces platensis NRRL 18993. The iso-MGS biosynthetic machinery featured an acy...
  13. Comparative Characterization of the Lactimidomycin and iso-Migrastatin Biosynthetic Machineries Revealing Unusual Features for Acyltransferase-less Type I Polyketide Synthases and Providing an Opportunity To Engineer New Analogues.

    Biochemistry (Washington) 53(49):7854 (2014) PMID 25405956 PMCID PMC4270375

    Lactimidomycin (LTM, 1) and iso-migrastatin (iso-MGS, 2) belong to the glutarimide-containing polyketide family of natural products. We previously cloned and characterized the mgs biosynthetic gene cluster from Streptomyces platensis NRRL 18993. The iso-MGS biosynthetic machinery featured an acy...
  14. Comparative characterization of the lactimidomycin and iso-migrastatin biosynthetic machineries revealing unusual features for acyltransferase-less type I polyketide synthases and providing an opportunity to engineer new analogues.

    Biochemistry (Washington) 53(49):7854 (2014) PMID 25405956 PMCID PMC4270375

    Lactimidomycin (LTM, 1) and iso-migrastatin (iso-MGS, 2) belong to the glutarimide-containing polyketide family of natural products. We previously cloned and characterized the mgs biosynthetic gene cluster from Streptomyces platensis NRRL 18993. The iso-MGS biosynthetic machinery featured an acy...
  15. Comparative Characterization of the Lactimidomycin and iso-Migrastatin Biosynthetic Machineries Revealing Unusual Features for Acyltransferase-less Type I Polyketide Synthases and Providing an Opportunity To Engineer New Analogues.

    Biochemistry (Washington) 53(49):7854 (2014) PMID 25405956 PMCID PMC4270375

    Lactimidomycin (LTM, 1) and iso-migrastatin (iso-MGS, 2) belong to the glutarimide-containing polyketide family of natural products. We previously cloned and characterized the mgs biosynthetic gene cluster from Streptomyces platensis NRRL 18993. The iso-MGS biosynthetic machinery featured an acy...
  16. A proteogenomics approach integrating proteomics and ribosome profiling increases the efficiency of protein identification and enables the discovery of alternative translation start sites.

    Proteomics 14(23-24):2688 (2014) PMID 25156699

    Next-generation transcriptome sequencing is increasingly integrated with MS to enhance MS-based protein and peptide identification. Recently, a breakthrough in transcriptome analysis was achieved with the development of ribosome profiling (ribo-seq). This technology is based on the deep sequenci...
  17. A proteogenomics approach integrating proteomics and ribosome profiling increases the efficiency of protein identification and enables the discovery of alternative translation start sites.

    Proteomics 14(23-24):2688 (2014) PMID 25156699 PMCID PMC4391000

    Next-generation transcriptome sequencing is increasingly integrated with MS to enhance MS-based protein and peptide identification. Recently, a breakthrough in transcriptome analysis was achieved with the development of ribosome profiling (ribo-seq). This technology is based on the deep sequenci...
  18. A proteogenomics approach integrating proteomics and ribosome profiling increases the efficiency of protein identification and enables the discovery of alternative translation start sites.

    Proteomics 14(23-24):2688 (2014) PMID 25156699

    Next-generation transcriptome sequencing is increasingly integrated with MS to enhance MS-based protein and peptide identification. Recently, a breakthrough in transcriptome analysis was achieved with the development of ribosome profiling (ribo-seq). This technology is based on the deep sequenci...
  19. A proteogenomics approach integrating proteomics and ribosome profiling increases the efficiency of protein identification and enables the discovery of alternative translation start sites.

    Proteomics 14(23-24):2688 (2014) PMID 25156699

    Next-generation transcriptome sequencing is increasingly integrated with MS to enhance MS-based protein and peptide identification. Recently, a breakthrough in transcriptome analysis was achieved with the development of ribosome profiling (ribo-seq). This technology is based on the deep sequenci...
  20. BlmB and TlmB provide resistance to the bleomycin family of antitumor antibiotics by N-acetylating metal-free bleomycin, tallysomycin, phleomycin, and zorbamycin.

    Biochemistry (Washington) 53(44):6901 (2014) PMID 25299801 PMCID PMC4230324

    The bleomycin (BLM) family of glycopeptide-derived antitumor antibiotics consists of BLMs, tallysomycins (TLMs), phleomycins (PLMs), and zorbamycin (ZBM). The self-resistant elements BlmB and TlmB, discovered from the BLM- and TLM-producing organisms Streptomyces verticillus ATCC15003 and Strept...