1. Antenatal betamethasone produces protracted changes in anxiety-like behaviors and in the expression of microtubule-associated protein 2, brain-derived neurotrophic factor and the tyrosine kinase B receptor in the rat cerebellar cortex.

    International Journal of Developmental Neurosci... 43:78 (2015) PMID 25889225

    Using classic Golgi staining methods, we previously showed that the administration of synthetic glucocorticoid betamethasone in equivalent doses to those given in cases of human premature birth generates long-term alterations in Purkinje cell dendritic development in the cerebellar cortex. In th...
  2. New insights into the regulatory mechanisms of ppGpp and DksA on Escherichia coli RNA polymerase-promoter complex.

    Nucleic Acids Research 43(10):5249 (2015) PMID 25916853 PMCID PMC4446441

    The stringent response modulators, guanosine tetraphosphate (ppGpp) and protein DksA, bind RNA polymerase (RNAP) and regulate gene expression to adapt bacteria to different environmental conditions. Here, we use Atomic Force Microscopy and in vitro transcription assays to study the effects of th...
  3. Ribosome. Mechanical force releases nascent chain-mediated ribosome arrest in vitro and in vivo.

    Science 348(6233):457 (2015) PMID 25908824

    Protein synthesis rates can affect gene expression and the folding and activity of the translation product. Interactions between the nascent polypeptide and the ribosome exit tunnel represent one mode of regulating synthesis rates. The SecM protein arrests its own translation, and release of arr...
  4. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways

    Cell 160(5):870 (2015)

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. −1 frameshifting occurs on Escherichia coli’s dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process...
  5. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways.

    Cell 160(5):870 (2015) PMID 25703095 PMCID PMC4344849

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. -1 frameshifting occurs on Escherichia coli's dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process, includin...
  6. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways.

    Cell 160(5):870 (2015) PMID 25703095 PMCID PMC4344849

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. -1 frameshifting occurs on Escherichia coli's dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process, includin...
  7. Ribosome Excursions during mRNA Translocation Mediate Broad Branching of Frameshift Pathways.

    Cell 160(5):870 (2015) PMID 25703095

    Programmed ribosomal frameshifting produces alternative proteins from a single transcript. -1 frameshifting occurs on Escherichia coli's dnaX mRNA containing a slippery sequence AAAAAAG and peripheral mRNA structural barriers. Here, we reveal hidden aspects of the frameshifting process, includin...
  8. Purkinje cell dendritic atrophy induced by prenatal stress is mitigated by early environmental enrichment.

    Neuropediatrics 46(1):37 (2015) PMID 25535699

    Prenatal stress (PS) in experimental animals causes long-lasting changes in Purkinje cell dendritic morphology. Furthermore, these structural changes are associated with an increase in anxiogenic behaviors in the elevated plus maze (EPM) and open-field (OF) test. As environmental enrichment (EE)...
  9. Purkinje cell dendritic atrophy induced by prenatal stress is mitigated by early environmental enrichment.

    Neuropediatrics 46(1):37 (2015) PMID 25535699

    Prenatal stress (PS) in experimental animals causes long-lasting changes in Purkinje cell dendritic morphology. Furthermore, these structural changes are associated with an increase in anxiogenic behaviors in the elevated plus maze (EPM) and open-field (OF) test. As environmental enrichment (EE)...
  10. Elucidating the Folding Pathways of Calcium-Binding Proteins

    Biophysical Journal 108(2):515a (2015)

  11. Role of Pore Loops in the Mechanism of Polypeptide Translocation by a AAA+ Protease Machine

    Biophysical Journal 108(2):502a (2015)

  12. Trigger loop folding determines transcription rate of Escherichia coli's RNA polymerase.

    PNAS 112(3):743 (2015) PMID 25552559 PMCID PMC4311812

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations mo...
  13. Trigger loop folding determines transcription rate of Escherichia coli's RNA polymerase.

    PNAS 112(3):743 (2015) PMID 25552559 PMCID PMC4311812

    Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations mo...
  14. Stochastic approach to the molecular counting problem in superresolution microscopy.

    PNAS 112(2):E110 (2015) PMID 25535361 PMCID PMC4299210

    Superresolution imaging methods-now widely used to characterize biological structures below the diffraction limit-are poised to reveal in quantitative detail the stoichiometry of protein complexes in living cells. In practice, the photophysical properties of the fluorophores used as tags in supe...
  15. Stochastic approach to the molecular counting problem in superresolution microscopy.

    PNAS 112(2):E110 (2015) PMID 25535361 PMCID PMC4299210

    Superresolution imaging methods--now widely used to characterize biological structures below the diffraction limit--are poised to reveal in quantitative detail the stoichiometry of protein complexes in living cells. In practice, the photophysical properties of the fluorophores used as tags in su...
  16. Stochastic approach to the molecular counting problem in superresolution microscopy.

    PNAS 112(2):E110 (2015) PMID 25535361

    Superresolution imaging methods-now widely used to characterize biological structures below the diffraction limit-are poised to reveal in quantitative detail the stoichiometry of protein complexes in living cells. In practice, the photophysical properties of the fluorophores used as tags in supe...
  17. Stochastic approach to the molecular counting problem in superresolution microscopy.

    PNAS 112(2):E110 (2015) PMID 25535361 PMCID PMC4299210

    Superresolution imaging methods--now widely used to characterize biological structures below the diffraction limit--are poised to reveal in quantitative detail the stoichiometry of protein complexes in living cells. In practice, the photophysical properties of the fluorophores used as tags in su...
  18. The heat released during catalytic turnover enhances the diffusion of an enzyme.

    Nature 517(7533):227 (2015) PMID 25487146

    Recent studies have shown that the diffusivity of enzymes increases in a substrate-dependent manner during catalysis. Although this observation has been reported and characterized for several different systems, the precise origin of this phenomenon is unknown. Calorimetric methods are often used...
  19. The heat released during catalytic turnover enhances the diffusion of an enzyme.

    Nature 517(7533):227 (2015) PMID 25487146 PMCID PMC4363105

    Recent studies have shown that the diffusivity of enzymes increases in a substrate-dependent manner during catalysis. Although this observation has been reported and characterized for several different systems, the precise origin of this phenomenon is unknown. Calorimetric methods are often used...
  20. The heat released during catalytic turnover enhances the diffusion of an enzyme.

    Nature 517(7533):227 (2015) PMID 25487146

    Recent studies have shown that the diffusivity of enzymes increases in a substrate-dependent manner during catalysis. Although this observation has been reported and characterized for several different systems, the precise origin of this phenomenon is unknown. Calorimetric methods are often used...