A novel multiplex bead-based platform highlights the diversity of extracellular vesicles.
Journal of Extracellular Vesicles 5:29975 (2016)
The surface protein composition of extracellular vesicles (EVs) is related to the originating cell and may play a role in vesicle function. Knowledge of the protein content of individual EVs is still limited because of the technical challenges to analyse small vesicles. Here, we introduce a nove...
A Synergistic Interaction between Chk1- and MK2 Inhibitors in KRAS-Mutant Cancer
Cell 162(5):1169 (2015)
A Synergistic Interaction between Chk1- and MK2 Inhibitors in KRAS-Mutant Cancer.
Cell 162(1):146 (2015)
KRAS is one of the most frequently mutated oncogenes in human cancer. Despite substantial efforts, no clinically applicable strategy has yet been developed to effectively treat KRAS-mutant tumors. Here, we perform a cell-line-based screen and identify strong synergistic interactions between cell...
IAP antagonization promotes inflammatory destruction of vascular endothelium.
EMBO Reports 16(6):719 (2015)
In this study, we show for the first time that the therapeutic antagonization of inhibitor of apoptosis proteins (IAPs) inhibits B16 melanoma growth by disrupting tumor vasculature. Specifically, the treatment of mice bearing B16 melanoma with an IAP antagonist compound A (Comp A) inhibits tumor...
miRs-138 and -424 control palmitoylation-dependent CD95-mediated cell death by targeting acyl protein thioesterases 1 and 2 in CLL.
Blood 125(19):2948 (2015)
Resistance toward CD95-mediated apoptosis is a hallmark of many different malignancies, as it is known from primary chronic lymphocytic leukemia (CLL) cells. Previously, we could show that miR-138 and -424 are downregulated in CLL cells. Here, we identified 2 new target genes, namely acyl protei...
Spastin binds to lipid droplets and affects lipid metabolism.
PLoS Genetics 11(4):e1005149 (2015)
Mutations in SPAST, encoding spastin, are the most common cause of autosomal dominant hereditary spastic paraplegia (HSP). HSP is characterized by weakness and spasticity of the lower limbs, owing to progressive retrograde degeneration of the long corticospinal axons. Spastin is a conserved micr...
Lipid droplets as a novel cargo of tunnelling nanotubes in endothelial cells.
Scientific reports 5:11453 (2015)
Intercellular communication is a fundamental process in the development and functioning of multicellular organisms. Recently, an essentially new type of intercellular communication, based on thin membrane channels between cells, has been reported. These structures, termed intercellular or tunnel...
Long-term live cell microscopy studies of lipid droplet fusion dynamics in adipocytes.
Journal of Lipid Research 54(12):3419 (2013)
During the adipogenic differentiation process of mesenchymal stem cells, lipid droplets (LDs) grow slowly by transferring lipids between each other. Recent findings hint at the possibility that a fusion pore is involved. In this study, we analyze lipid transfer data obtained in long-term label-f...
Two-color glycan labeling of live cells by a combination of Diels-Alder and click chemistry.
Angewandte Chemie. International edition in Eng... 52(15):4265 (2013)
Locally resolved membrane binding affinity of the N-terminus of α-synuclein.
Biochemistry (Washington) 51(19):3960 (2012)
α-Synuclein is abundantly present in Lewy bodies, characteristic of Parkinson's disease. Its exact physiological role has yet to be determined, but mitochondrial membrane binding is suspected to be a key aspect of its function. Electron paramagnetic resonance spectroscopy in combination with sit...
Remodeling of lipid droplets during lipolysis and growth in adipocytes.
Journal of Biological Chemistry 287(14):11164 (2012)
Synthesis, storage, and turnover of triacylglycerols (TAGs) in adipocytes are critical cellular processes to maintain lipid and energy homeostasis in mammals. TAGs are stored in metabolically highly dynamic lipid droplets (LDs), which are believed to undergo fragmentation and fusion under lipoly...
Fluorescent conjugated block copolymer nanoparticles by controlled mixing.
Chemical Communications 48(15):2104 (2012)
Monitoring of the formation of stable fluorescent nanoparticles from controlled mixing of a THF solution of poly(fluorene ethynylene)-block-poly(ethylene glycol) in a microfluidic laminar flow crossjunction by spatially resolved fluorescence spectroscopy reveals the time scale of particle format...
Fast and long term lipid droplet tracking with CARS microscopy.
Journal of Biophotonics 4(6):435 (2011)
Photobleaching of organic fluorophores commonly used in fluorescence microscopy puts a limit to the number of images which can be acquired. Label-free imaging techniques therefore offer advantages both for rapid image acquisition and for long-term observations. CARS microscopy is a label-free im...
Asymmetric PPCys: strongly fluorescing NIR labels.
Chemical Communications 46(29):5289 (2010)
By a stepwise synthesis strategy biofunctionalized Pyrrolopyrrole Cyanines (PPCy) with an asymmetric substitution pattern were obtained. These exhibit extremely strong and narrowband NIR absorption and fluorescence. Internalization of a peptide bound PPCy is demonstrated using live cell microsco...