Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.
Journal of Experimental Medicine 213(6):979 (2016)
Hematopoiesis culminates in the production of functionally heterogeneous blood cell types. In zebrafish, the lack of cell surface antibodies has compelled researchers to use fluorescent transgenic reporter lines to label specific blood cell fractions. However, these approaches are limited by the...
Uncharted Waters: Zebrafish Cancer Models Navigate a Course for Oncogene Discovery.
Advances in Experimental Medicine and Biology 916:3 (2016)
Over a decade has elapsed since the first genetically-engineered zebrafish cancer model was described. During this time remarkable progress has been made. Sophisticated genetic tools have been built to generate oncogene expressing cancers and characterize multiple models of solid and blood tumor...
Melanoma Regression and Recurrence in Zebrafish.
Methods in Molecular Biology 1451:143 (2016)
Melanoma is the most lethal form of skin cancer with high mortality rates. Most melanoma cases have activating mutations in BRAF (V600E) and the selective inhibitors of BRAF(V600E) have been successfully used in patients. However, after initial tumor regression, the majority of patients develop ...
Poised Regeneration of Zebrafish Melanocytes Involves Direct Differentiation and Concurrent Replenishment of Tissue-Resident Progenitor Cells.
Developmental Cell 33(6):631 (2015)
Efficient regeneration following injury is critical for maintaining tissue function and enabling organismal survival. Cells reconstituting damaged tissue are often generated from resident stem or progenitor cells or from cells that have dedifferentiated and become proliferative. While lineage-tr...
Zebrafish as a platform to study tumor progression.
Methods in Molecular Biology 1176:143 (2014)
The zebrafish has emerged as a powerful model system to study human diseases, including a variety of neoplasms. Principal components that have contributed to the rise in use of this vertebrate model system are its high fecundity, ease of genetic manipulation, and low cost of maintenance. Vital i...
Screening for melanoma modifiers using a zebrafish autochthonous tumor model.
Journal of Visualized Experiments (69):e50086 (2012)
Genomic studies of human cancers have yielded a wealth of information about genes that are altered in tumors. A challenge arising from these studies is that many genes are altered, and it can be difficult to distinguish genetic alterations that drove tumorigenesis from that those arose incidenta...
The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset.
Nature 471(7339):513 (2011)
The most common mutation in human melanoma, BRAF(V600E), activates the serine/threonine kinase BRAF and causes excessive activity in the mitogen-activated protein kinase pathway. BRAF(V600E) mutations are also present in benign melanocytic naevi, highlighting the importance of additional genetic...
Melanoma biology and the promise of zebrafish.
Zebrafish 5(4):247 (2008)
Advantageous organismal and technical attributes of the zebrafish are being increasingly applied to study cancer biology. Along with other tumor models, zebrafish that develop melanomas have been generated. In both genetics and phenotype, zebrafish melanomas are strikingly similar to their human...
APC and colon cancer: two hits for one.
Nature Medicine 13(11):1286 (2007)
Some C. elegans class B synthetic multivulva proteins encode a conserved LIN-35 Rb-containing complex distinct from a NuRD-like complex.
PNAS 103(45):16782 (2006)
The Caenorhabditis elegans synthetic multivulva (synMuv) genes act redundantly to antagonize the specification of vulval cell fates, which are promoted by an RTK/Ras pathway. At least 26 synMuv genes have been genetically identified, several of which encode proteins with homologs that act in chr...
Identification and classification of genes that act antagonistically to let-60 Ras signaling in Caenorhabditis elegans vulval development.
Genetics 173(2):709 (2006)
The synthetic multivulva (synMuv) genes negatively regulate Ras-mediated vulval induction in the nematode Caenorhabditis elegans. The synMuv genes define three classes, A, B, and C, such that double mutants carrying mutations in genes of any two classes are multivulva. The class B synMuv genes i...
A new class of C. elegans synMuv genes implicates a Tip60/NuA4-like HAT complex as a negative regulator of Ras signaling.
Developmental Cell 6(4):563 (2004)
The class A and class B synMuv genes are functionally redundant negative regulators of a Ras signaling pathway that induces C. elegans vulval development. A number of class B synMuv genes encode components of an Rb and histone deacetylase complex that likely acts to repress transcription of gene...
A New Class ofC. eleganssynMuv Genes Implicates a Tip60/NuA4-like HAT Complex as a Negative Regulator of Ras Signaling
Developmental Cell 6(4):563 (2004)
The class A and class B synMuv genes are functionally redundant negative regulators of a Ras signaling pathway that induces
C. elegans vulval development. A number of class B synMuv genes encode components of an Rb and histone deacetylase complex that likely acts to repress transcrip...
New genes that interact with lin-35 Rb to negatively regulate the let-60 ras pathway in Caenorhabditis elegans.
Genetics 164(1):135 (2003)
Previous studies have shown that a synthetic multivulva phenotype results from mutations in genes that antagonize the ras-mediated intercellular signaling system responsible for vulval induction in Caenorhabditis elegans. Synthetic multivulva mutations define two classes of genes, A and B, and a...