An ultrasensitive squamous cell carcinoma antigen biosensing platform utilizing double-antibody single-channel amplification strategy.
Biosensors and Bioelectronics 72:156 (2015)
A novel electrochemical immunosensor was developed for ultrasensitive detection of squamous cell carcinoma antigen (SCCA), which was based on the double-antibody single-channel amplification strategy. For the first time, human immunoglobulin antibody (anti-HIgG) was used as the supporting framew...
Electrochemiluminescence modified electrodes based on RuSi@Ru(bpy)3(2+) loaded with gold functioned nanoporous CO/Co3O4 for detection of mycotoxin deoxynivalenol.
Biosensors and Bioelectronics 70:28 (2015)
Nanoporous cobalt (NPCo) with three-dimensional network nanostructure was obtained by selectively dealloying Al from CoAl alloy in a mild alkaline solution. Co3O4 grew further on the surface of NPCo and then NPCo/Co3O4 was obtained. Gold nanoparticles functionalized NPCo/Co3O4 (NPCo/Co3O4-Au) wa...
Desulfurization of immobilized sulfur-oxidizing bacteria, Thialkalivibrio versutus, by magnetic nanaoparticles under haloalkaliphilic conditions.
Biotechnology Letters 37(8):1631 (2015)
As a haloalkaliphilic, sulfur-oxidizing bacteria, Thialkalivibrio versutus D301 can remove sulfide, thiosulfate and polysulfide in wastewater, we investigated how it might be reused when mixed with high concentrations of elemental sulfur.
A process is described to immobilize T. versutus cells by...
A simple, rapid and reliable liquid chromatography-mass spectrometry method for determination of methotrexate in human plasma and its application to therapeutic drug monitoring.
Biomedical Chromatography 29(8):1197 (2015)
A simple, rapid and reliable liquid chromatography-electrospray ionization tandem mass spectrometry method was established and validated for the determination of methotrexate in human plasma. After a straightforward protein precipitation by acetonitrile-water (70:30, v/v), methotrexate (MTX) and...
Salidroside ameliorates insulin resistance through activation of a mitochondria-associated AMPK/PI3K/Akt/GSK3β pathway.
British journal of pharmacology and chemotherapy 172(13):3284 (2015)
Recent reports have suggested that salidroside could protect cardiomyocytes from oxidative injury and stimulate glucose uptake in skeletal muscle cells by activating AMP-activated protein kinase (AMPK). The aim of this study was to evaluate the therapeutic effects of salidroside on diabetic mice...
C1q/tumor necrosis factor-related protein-6 attenuates post-infarct cardiac fibrosis by targeting RhoA/MRTF-A pathway and inhibiting myofibroblast differentiation.
Archiv fÃ¼r Kreislaufforschung 110(4):35 (2015)
C1q/tumor necrosis factor-related protein-6 (CTRP6) is a newly identified adiponectin paralog with modulation effects on metabolism and inflammation. However, the cardiovascular function of CTRP6 remains unknown. This study aimed to determine its role in cardiac fibrosis and explore the possible...
Population genetic data of 15 STR loci in Gansu Han population from China.
International Journal of Legal Medicine 129(4):735 (2015)
The genetic polymorphisms of 15 autosomal short tandem repeat (STR) loci included in the AmpFISTR Sinofiler™ kit were evaluated in 547 healthy unrelated Han individuals from Gansu, China. All of the loci reached the Hardy-Weinberg equilibrium after the Bonferroni correction (p > 0.0033). These l...
The role of the TLR4/NF-κB signaling pathway in Aβ accumulation in primary hippocampal neurons.
Sheng li xue bao : [Acta physiologica Sinica] 67(3):319 (2015)
The present study aimed to investigate the role of the Toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB) signaling pathway in the accumulation of amyloid β protein (Aβ) in primary hippocampal neurons of rats. The purity of these cultured neurons was determined by using immunofluorescence tec...
Application of Europium Multiwalled Carbon Nanotubes as Novel Luminophores in an Electrochemiluminescent Aptasensor for Thrombin Using Multiple Amplification Strategies.
ACS Applied Materials & Interfaces 7(23):12663 (2015)
A novel electrochemiluminescent (ECL) aptasensor was proposed for the determination of thrombin (TB) using exonuclease-catalyzed target recycling and hybridization chain reaction (HCR) to amplify the signal. The capture probe was immobilized on an Au-GS-modified electrode through a Au-S bond. Su...
Compact, Programmable, and Stable Biofunctionalized Upconversion Nanoparticles Prepared through Peptide-Mediated Phase Transfer for High-Sensitive Protease Sensing and in Vivo Apoptosis Imaging.
ACS Applied Materials & Interfaces 7(22):11849 (2015)
Protease represents an important class of biomarkers for disease diagnostics and drug screening. Conventional fluorescence-based probes for in vivo protease imaging suffer from short excitation wavelengths and poor photostability. Upconversion nanoparticles (UCNPs) hold great promise for biosens...
Design of a brightness-enhancement-film-adaptive freeform lens to enhance overall performance in direct-lit light-emitting diode backlighting.
Applied Optics 54(17):5542 (2015)
In this study, a brightness-enhancement-film- (BEF) adaptive method is proposed to design freeform lenses for enhancing brightness performance in a direct-lit light-emitting diode (LED) backlight system. A detailed design algorithm is presented based on the analysis of the output optical propert...
Incomplete Kawasaki disease with coronary artery aneurysm and coronary sinus thrombus.
Pediatric Cardiology 36(5):1097 (2015)
We describe herein a rare case of coronary artery aneurysm and coronary sinus thrombus due to incomplete Kawasaki disease. To the best of our knowledge, coronary sinus thrombosis has not previously been reported as a complication of this disease. Our patient underwent thrombolytic therapy with a...
Mechanism of miR-21 via Wnt/β-catenin signaling pathway in human A549 lung cancer cells and Lewis lung carcinoma in mice.
Asian Pacific Journal of Tropical Medicine 8(6):479 (2015)
To study the mechanism of effect of miR-21 via Wnt/β-catenin signaling pathway in human A549 lung cancer cells and Lewis lung carcinoma in mice.
The effect of miR-21 on A549 cells were detected by MTT method. MiR-21 expression levels were overexpressed or inhibited in A549 cells by transfecting ...
Effects of insulin-like growth factor-1 on the relaxation responses of the cavernous smooth muscle from aged rats.
Scandinavian Journal of Urology 49(3):260 (2015)
The aim of this study was determine whether intracavernosal injection (ICI) of insulin-like growth factor-1 (IGF-1) protein can improve corpus cavernosal smooth muscle relaxation in aging rats.
Ten young (4-month-old) and 30 old (24-month-old) Sprague-Dawley male rats were enrolled in the study....
IL-33 Reverses an Obesity-Induced Deficit in Visceral Adipose Tissue ST2+ T Regulatory Cells and Ameliorates Adipose Tissue Inflammation and Insulin Resistance.
Journal of Immunology 194(10):4777 (2015)
Obesity is associated with insulin resistance and inflammation thought to be caused by a visceral adipose tissue (VAT)-localized reduction in immunoregulatory cells and increase in proinflammatory immune cells. We previously found that VAT regulatory T cells (Tregs) normally express high levels ...
[Value of cervical liquid-based cytology and high-risk human papilloma virus for patients of high-grade squamous intraepithelial lesion excised with positive margins by loop electrosurgical excision procedure].
Zhonghua Yixue Zazhi 95(17):1331 (2015)
To explore the value of cervical liquid-based cytology test (LCT) and high-risk human papilloma virus for patients of high-grade squamous intraepithelial lesion (HSIL) excised with positive margins by loop electrosurgical excision procedure (LEEP).
A total of 404 HSIL patients with positive marg...
Advanced principal component analysis method for phase reconstruction.
Optics Express 23(9):12222 (2015)
Focus on the phase reconstruction from three phase-shifting interferograms with unknown phase shifts, an advanced principal component analysis method is proposed. First, use a simple subtraction operation among interferograms, two intensity difference images are obtained easily. Second, set the ...
Early-Onset Candida glabrata Interface Keratitis after Deep Anterior Lamellar Keratoplasty.
Optometry and Vision Science 92(5):e93 (2015)
Fungal interface keratitis by Candida species can occur several weeks to several months after deep anterior lamellar keratoplasty (DALK). Here, we report a case of early-onset fungal interface keratitis by Candida glabrata after DALK.
A 31-year-old Chinese man complained of decreased vision 4 da...
Kinetics of plasma microRNA-499 expression in acute myocardial infarction.
Journal of thoracic disease 7(5):890 (2015)
MicroRNA (miRNA) is reported to be present in human plasma and has been increasingly suggested as a biomarker for diseases. Our study aimed to investigate the kinetics of cardiac-specific microR-499 (miR-499) in acute myocardial infarction (AMI).
Circulating concentrations of cardiac enriched mi...
p-Benzoquinone adsorption-separation, sensing and its photoinduced transformation within a robust Cd(II)-MOF in a SC-SC fashion.
Chemical Communications 51(35):7443 (2015)
p-Benzoquinone (Q) adsorption–separation, sensing and its photoinduced transformation within a robust Cd(II)-MOF (1) is reported. All the adsorption, sensing and photochemical reactions are directly performed on the single-crystals of 1.