1. Fitness cost of antibiotic susceptibility during bacterial infection.

    Science Translational Medicine 7(297):297ra114 (2015) PMID 26203082

    Advances in high-throughput DNA sequencing allow for a comprehensive analysis of bacterial genes that contribute to virulence in a specific infectious setting. Such information can yield new insights that affect decisions on how to best manage major public health issues such as the threat posed ...
  2. A Poly-N-acetylglucosamine-Shiga toxin broad-spectrum conjugate vaccine for Shiga toxin-producing Escherichia coli.

    mBio 5(2):e00974 (2014) PMID 24667709 PMCID PMC3977355

    Many pathogens produce the β-(1-6)-linked poly-N-acetylglucosamine (PNAG) surface polysaccharide that is being developed as a broadly protective antimicrobial vaccine. However, it is unknown whether systemically injected PNAG vaccines or antibodies would provide protective immunity against patho...
  3. Enhanced in vivo fitness of carbapenem-resistant oprD mutants of Pseudomonas aeruginosa revealed through high-throughput sequencing.

    PNAS 110(51):20747 (2013) PMID 24248354 PMCID PMC3870709

    An important question regarding the biologic implications of antibiotic-resistant microbes is how resistance impacts the organism's overall fitness and virulence. Currently it is generally thought that antibiotic resistance carries a fitness cost and reduces virulence. For the human pathogen Pse...
  4. Enhanced in vivo fitness of carbapenem-resistant oprD mutants of Pseudomonas aeruginosa revealed through high-throughput sequencing.

    PNAS 110(51):20747 (2013) PMID 24248354 PMCID PMC3870709

    An important question regarding the biologic implications of antibiotic-resistant microbes is how resistance impacts the organism's overall fitness and virulence. Currently it is generally thought that antibiotic resistance carries a fitness cost and reduces virulence. For the human pathogen Pse...
  5. Airway fungal colonization compromises the immune system allowing bacterial pneumonia to prevail.

    Critical Care Medicine 41(9):e191 (2013) PMID 23887232

    To study the correlation between fungal colonization and bacterial pneumonia and to test the effect of antifungal treatments on the development of bacterial pneumonia in colonized rats. Experimental animal investigation. University research laboratory. Pathogen-free male Wistar rats weighing 250...
  6. Airway fungal colonization compromises the immune system allowing bacterial pneumonia to prevail.

    Critical Care Medicine 41(9):e191 (2013) PMID 23887232

    To study the correlation between fungal colonization and bacterial pneumonia and to test the effect of antifungal treatments on the development of bacterial pneumonia in colonized rats. Experimental animal investigation. University research laboratory. Pathogen-free male Wistar rats weighing 250...
  7. Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens.

    PNAS 110(24):E2209 (2013) PMID 23716675 PMCID PMC3683766

    Microbial capsular antigens are effective vaccines but are chemically and immunologically diverse, resulting in a major barrier to their use against multiple pathogens. A β-(1→6)-linked poly-N-acetyl-d-glucosamine (PNAG) surface capsule is synthesized by four proteins encoded in genetic loci des...
  8. Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens.

    PNAS 110(24):E2209 (2013) PMID 23716675 PMCID PMC3683766

    Microbial capsular antigens are effective vaccines but are chemically and immunologically diverse, resulting in a major barrier to their use against multiple pathogens. A β-(1→6)-linked poly-N-acetyl-d-glucosamine (PNAG) surface capsule is synthesized by four proteins encoded in genetic loci des...
  9. Commensal Escherichia coli strains in Guiana reveal a high genetic diversity with host-dependant population structure.

    Environmental Microbiology Reports 5(1):49 (2013) PMID 23757130

    We undertook a large-scale epidemiological survey of commensal Escherichia coli in Trois-Sauts, an isolated village located in the south of French Guiana where human population exchanges are restricted and source of antibiotics controlled. Stools from 162 Wayampi Amerindians and rectal swabs fro...
  10. Commensal Escherichia coli strains in Guiana reveal a high genetic diversity with host-dependant population structure.

    Environmental Microbiology Reports 5(1):49 (2013) PMID 23757130

    We undertook a large-scale epidemiological survey of commensal Escherichia coli in Trois-Sauts, an isolated village located in the south of French Guiana where human population exchanges are restricted and source of antibiotics controlled. Stools from 162 Wayampi Amerindians and rectal swabs fro...
  11. Randomized controlled trial of parent therapeutic education on antibiotics to improve parent satisfaction and attitudes in a pediatric emergency department.

    PLoS ONE 8(9):e75590 (2013) PMID 24086581 PMCID PMC3784452

    To evaluate therapeutic education delivered in a pediatric emergency department to improve parents' satisfaction and attitudes about judicious antibiotic use. In an emergency department of a tertiary pediatric hospital, children aged 1 month to 6 years and discharged with an oral antibiotic pres...
  12. Randomized controlled trial of parent therapeutic education on antibiotics to improve parent satisfaction and attitudes in a pediatric emergency department.

    PLoS ONE 8(9):e75590 (2013) PMID 24086581 PMCID PMC3784452

    To evaluate therapeutic education delivered in a pediatric emergency department to improve parents' satisfaction and attitudes about judicious antibiotic use. In an emergency department of a tertiary pediatric hospital, children aged 1 month to 6 years and discharged with an oral antibiotic pres...
  13. A comprehensive analysis of in vitro and in vivo genetic fitness of Pseudomonas aeruginosa using high-throughput sequencing of transposon libraries.

    PLoS Pathogens 9(9):e1003582 (2013) PMID 24039572 PMCID PMC3764216

    High-throughput sequencing of transposon (Tn) libraries created within entire genomes identifies and quantifies the contribution of individual genes and operons to the fitness of organisms in different environments. We used insertion-sequencing (INSeq) to analyze the contribution to fitness of a...
  14. A comprehensive analysis of in vitro and in vivo genetic fitness of Pseudomonas aeruginosa using high-throughput sequencing of transposon libraries.

    PLoS Pathogens 9(9):e1003582 (2013) PMID 24039572 PMCID PMC3764216

    High-throughput sequencing of transposon (Tn) libraries created within entire genomes identifies and quantifies the contribution of individual genes and operons to the fitness of organisms in different environments. We used insertion-sequencing (INSeq) to analyze the contribution to fitness of a...
  15. Magic bullets for the 21st century: the reemergence of immunotherapy for multi- and pan-resistant microbes.

    Journal of Antimicrobial Chemotherapy 67(12):2785 (2012) PMID 22899807 PMCID PMC3695597

    In our current world, antibiotic resistance among pathogenic microbes keeps getting worse with few new antibiotics being pursued by pharmaceutical companies. Modern-day immunotherapies, reminiscent of the serotherapy approaches used in the early days of antimicrobial treatments, are a potential ...
  16. Magic bullets for the 21st century: the reemergence of immunotherapy for multi- and pan-resistant microbes.

    Journal of Antimicrobial Chemotherapy 67(12):2785 (2012) PMID 22899807 PMCID PMC3695597

    In our current world, antibiotic resistance among pathogenic microbes keeps getting worse with few new antibiotics being pursued by pharmaceutical companies. Modern-day immunotherapies, reminiscent of the serotherapy approaches used in the early days of antimicrobial treatments, are a potential ...
  17. Natural antibodies in normal human serum inhibit Staphylococcus aureus capsular polysaccharide vaccine efficacy.

    Clinical Infectious Diseases 55(9):1188 (2012) PMID 22806596 PMCID PMC3529611

    Vaccines against Streptococcus pneumoniae, Neisseria meningitidis, and Hemophilus influenzae type b induce functional opsonic or bactericidal antibodies to surface capsular polysaccharides (CP). Targeting the comparable Staphylococcus aureus CP seems logical, but to date such efforts have failed...
  18. Natural antibodies in normal human serum inhibit Staphylococcus aureus capsular polysaccharide vaccine efficacy.

    Clinical Infectious Diseases 55(9):1188 (2012) PMID 22806596 PMCID PMC3529611

    Vaccines against Streptococcus pneumoniae, Neisseria meningitidis, and Hemophilus influenzae type b induce functional opsonic or bactericidal antibodies to surface capsular polysaccharides (CP). Targeting the comparable Staphylococcus aureus CP seems logical, but to date such efforts have failed...
  19. Targeting pan-resistant bacteria with antibodies to a broadly conserved surface polysaccharide expressed during infection.

    Journal of Infectious Diseases 205(11):1709 (2012) PMID 22448004 PMCID PMC3415848

    New therapeutic targets for antibiotic-resistant bacterial pathogens are desperately needed. The bacterial surface polysaccharide poly-β-(1-6)-N-acetyl-glucosamine (PNAG) mediates biofilm formation by some bacterial species, and antibodies to PNAG can confer protective immunity. By analyzing seq...
  20. Targeting pan-resistant bacteria with antibodies to a broadly conserved surface polysaccharide expressed during infection.

    Journal of Infectious Diseases 205(11):1709 (2012) PMID 22448004 PMCID PMC3415848

    New therapeutic targets for antibiotic-resistant bacterial pathogens are desperately needed. The bacterial surface polysaccharide poly-β-(1-6)-N-acetyl-glucosamine (PNAG) mediates biofilm formation by some bacterial species, and antibodies to PNAG can confer protective immunity. By analyzing seq...