1. Amplification of TRIM44: pairing a prognostic target with potential therapeutic strategy.

    JNCI Journal of the National Cancer Institute 106(5) (2014) PMID 24777112 PMCID PMC4112924

    Many prognostic biomarkers have been proposed recently. However, there is a lack of therapeutic strategies exploiting novel prognostic biomarkers. We aimed to propose therapeutic options in patients with overexpression of TRIM44, a recently identified prognostic gene. Genomic and transcriptomic ...
  2. Amplification of TRIM44: pairing a prognostic target with potential therapeutic strategy.

    JNCI Journal of the National Cancer Institute 106(5) (2014) PMID 24777112 PMCID PMC4112924

    Many prognostic biomarkers have been proposed recently. However, there is a lack of therapeutic strategies exploiting novel prognostic biomarkers. We aimed to propose therapeutic options in patients with overexpression of TRIM44, a recently identified prognostic gene. Genomic and transcriptomic ...
  3. Dual-modality gene reporter for in vivo imaging.

    PNAS 111(1):415 (2014) PMID 24347640 PMCID PMC3890795

    The ability to track cells and their patterns of gene expression in living organisms can increase our understanding of tissue development and disease. Gene reporters for bioluminescence, fluorescence, radionuclide, and magnetic resonance imaging (MRI) have been described but these suffer various...
  4. Magnetic resonance imaging of tumor glycolysis using hyperpolarized 13C-labeled glucose.

    Nature Medicine 20(1):93 (2014) PMID 24317119 PMCID PMC3886895

    In this study, we monitored glycolysis in mouse lymphoma and lung tumors by measuring the conversion of hyperpolarized [U-2H, U-13C]glucose to lactate using 13C magnetic resonance spectroscopy and spectroscopic imaging. We observed labeled lactate only in tumors and not in surrounding normal tis...
  5. Analysis of image heterogeneity using 2D Minkowski functionals detects tumor responses to treatment.

    Magnetic Resonance in Medicine 71(1):402 (2014) PMID 23440731

    The acquisition of ever increasing volumes of high resolution magnetic resonance imaging (MRI) data has created an urgent need to develop automated and objective image analysis algorithms that can assist in determining tumor margins, diagnosing tumor stage, and detecting treatment response. We h...
  6. Magnetic resonance imaging of tumor glycolysis using hyperpolarized 13C-labeled glucose.

    Nature Medicine 20(1):93 (2014) PMID 24317119 PMCID PMC3886895

    In this study, we monitored glycolysis in mouse lymphoma and lung tumors by measuring the conversion of hyperpolarized [U-2H, U-13C]glucose to lactate using 13C magnetic resonance spectroscopy and spectroscopic imaging. We observed labeled lactate only in tumors and not in surrounding normal tis...
  7. Spin echo measurements of the extravasation and tumor cell uptake of hyperpolarized [1-(13) C]lactate and [1-(13) C]pyruvate.

    Magnetic Resonance in Medicine 70(5):1200 (2013) PMID 23280500

    To assess the blood-tissue distribution of hyperpolarized (13) C-labeled molecules in vivo. Spin-echo experiments with simultaneous acquisition of the free induction decay (FID) signal following the excitation pulse and the spin-echo signal, were used to monitor hyperpolarized [1-(13) C]lactate,...
  8. Magnetic resonance imaging with hyperpolarized [1,4-(13)C2]fumarate allows detection of early renal acute tubular necrosis.

    PNAS 109(33):13374 (2012) PMID 22837393 PMCID PMC3421192

    Acute kidney injury (AKI) is a common and important medical problem, affecting 10% of hospitalized patients, and it is associated with significant morbidity and mortality. The most frequent cause of AKI is acute tubular necrosis (ATN). Current imaging techniques and biomarkers do not allow ATN t...
  9. Probing lactate dehydrogenase activity in tumors by measuring hydrogen/deuterium exchange in hyperpolarized l-[1-(13)C,U-(2)H]lactate.

    Journal of the American Chemical Society 134(10):4969 (2012) PMID 22316419 PMCID PMC3303201

    (13)C magnetic resonance spectroscopy and spectroscopic imaging measurements of hyperpolarized (13)C label exchange between exogenously administered [1-(13)C]pyruvate and endogenous lactate, catalyzed by lactate dehydrogenase (LDH), has proved to be a powerful approach for probing tissue metabol...
  10. Hyperpolarized (13)C spectroscopy detects early changes in tumor vasculature and metabolism after VEGF neutralization.

    Cancer Research 72(4):854 (2012) PMID 22223844 PMCID PMC3378497

    No clinically validated biomarkers exist to image tumor responses to antiangiogenic therapy. Here, we report the utility of hyperpolarized (13)C magnetic resonance spectroscopy (MRS) to detect the early effects of anti-VEGF therapy. In two colorectal cancer xenograft models, displaying different...
  11. Hyperpolarized [1-13C]-ascorbic and dehydroascorbic acid: vitamin C as a probe for imaging redox status in vivo.

    Journal of the American Chemical Society 133(30):11795 (2011) PMID 21692446 PMCID PMC3144679

    Dynamic nuclear polarization (DNP) of (13)C-labeled metabolic substrates in vitro and their subsequent intravenous administration allow both the location of the hyperpolarized substrate and the dynamics of its subsequent conversion into other metabolic products to be detected in vivo. We report ...
  12. Detection of tumor glutamate metabolism in vivo using (13)C magnetic resonance spectroscopy and hyperpolarized [1-(13)C]glutamate.

    Magnetic Resonance in Medicine 66(1):18 (2011) PMID 21695718

    Dynamic nuclear polarization can be used to increase the sensitivity of solution state (13)C magnetic resonance spectroscopy by four orders of magnitude. We show here that [1-(13)C]glutamate can be polarized to 28%, representing a 35,000-fold increase in its sensitivity to detection at 9.4 T and...
  13. Acute NADPH oxidase activation potentiates cerebrovascular permeability response to bradykinin in ischemia-reperfusion.

    Free Radical Biology and Medicine 50(4):518 (2011) PMID 21167936 PMCID PMC3038265

    Free radical generation is a key event in cerebral reperfusion injury. Bradykinin (Bk) and interleukin-1β (IL-1β) have both been implicated in edema formation after stroke, although acute Bk application itself results in only a modest permeability increase. We have investigated the molecular mec...
  14. Acute NADPH oxidase activation potentiates cerebrovascular permeability response to bradykinin in ischemia–reperfusion

    Free Radical Biology and Medicine 50(4):518 (2011)

    Free radical generation is a key event in cerebral reperfusion injury. Bradykinin (Bk) and interleukin-1β (IL-1β) have both been implicated in edema formation after stroke, although acute Bk application itself results in only a modest permeability increase. We have investigated the molecula...
  15. Detection of tumor response to a vascular disrupting agent by hyperpolarized 13C magnetic resonance spectroscopy.

    Molecular Cancer Therapeutics 9(12):3278 (2010) PMID 21159611 PMCID PMC3003424

    Nuclear spin hyperpolarization can dramatically increase the sensitivity of the (13)C magnetic resonance experiment, allowing dynamic measurements of the metabolism of hyperpolarized (13)C-labeled substrates in vivo. Here, we report a preclinical study of the response of lymphoma tumors to the v...
  16. Detection of tumor response to a vascular disrupting agent by hyperpolarized 13C magnetic resonance spectroscopy.

    Molecular Cancer Therapeutics 9(12):3278 (2010) PMID 21159611 PMCID PMC3003424

    Nuclear spin hyperpolarization can dramatically increase the sensitivity of the (13)C magnetic resonance experiment, allowing dynamic measurements of the metabolism of hyperpolarized (13)C-labeled substrates in vivo. Here, we report a preclinical study of the response of lymphoma tumors to the v...
  17. Detection of tumor response to a vascular disrupting agent by hyperpolarized 13C magnetic resonance spectroscopy.

    Molecular Cancer Therapeutics 9(12):3278 (2010) PMID 21159611 PMCID PMC3003424

    Nuclear spin hyperpolarization can dramatically increase the sensitivity of the (13)C magnetic resonance experiment, allowing dynamic measurements of the metabolism of hyperpolarized (13)C-labeled substrates in vivo. Here, we report a preclinical study of the response of lymphoma tumors to the v...
  18. Detection of tumor response to a vascular disrupting agent by hyperpolarized 13C magnetic resonance spectroscopy.

    Molecular Cancer Therapeutics 9(12):3278 (2010) PMID 21159611 PMCID PMC3003424

    Nuclear spin hyperpolarization can dramatically increase the sensitivity of the (13)C magnetic resonance experiment, allowing dynamic measurements of the metabolism of hyperpolarized (13)C-labeled substrates in vivo. Here, we report a preclinical study of the response of lymphoma tumors to the v...
  19. Detection of tumor response to a vascular disrupting agent by hyperpolarized 13C magnetic resonance spectroscopy.

    Molecular Cancer Therapeutics 9(12):3278 (2010) PMID 21159611 PMCID PMC3003424

    Nuclear spin hyperpolarization can dramatically increase the sensitivity of the (13)C magnetic resonance experiment, allowing dynamic measurements of the metabolism of hyperpolarized (13)C-labeled substrates in vivo. Here, we report a preclinical study of the response of lymphoma tumors to the v...
  20. Detection of tumor response to a vascular disrupting agent by hyperpolarized 13C magnetic resonance spectroscopy.

    Molecular Cancer Therapeutics 9(12):3278 (2010) PMID 21159611 PMCID PMC3003424

    Nuclear spin hyperpolarization can dramatically increase the sensitivity of the (13)C magnetic resonance experiment, allowing dynamic measurements of the metabolism of hyperpolarized (13)C-labeled substrates in vivo. Here, we report a preclinical study of the response of lymphoma tumors to the v...