1. Understanding the mechanism of short-range electron transfer using an immobilized cupredoxin.

    Journal of the American Chemical Society 134(29):11848 (2012) PMID 22788731

    The hydrophobic patch of azurin (AZ) from Pseudomonas aeruginosa is an important recognition surface for electron transfer (ET) reactions. The influence of changing the size of this region, by mutating the C-terminal copper-binding loop, on the ET reactivity of AZ adsorbed on gold electrodes mod...
  2. The influence of active site loop mutations on the thermal stability of azurin from Pseudomonas aeruginosa.

    Archives of Biochemistry and Biophysics 521(1-2):18 (2012) PMID 22446157

    The copper site and overall structures of azurin (AZ) variants in which the amicyanin (AMI) and plastocyanin (PC) metal binding loops have been introduced, AZAMI and AZPC, respectively, are similar to that of AZ, whereas the loop conformations resemble those in the native proteins. To assess the...
  3. Redox cycling and kinetic analysis of single molecules of solution-phase nitrite reductase.

    PNAS 108(42):17269 (2011) PMID 21969548 PMCID PMC3198337

    Single-molecule measurements are a valuable tool for revealing details of enzyme mechanisms by enabling observation of unsynchronized behavior. However, this approach often requires immobilizing the enzyme on a substrate, a process which may alter enzyme behavior. We apply a microfluidic trappin...
  4. Fluorescence lifetime analysis of nitrite reductase from Alcaligenes xylosoxidans at the single-molecule level reveals the enzyme mechanism.

    Chemistry: A European Journal 17(43):12015 (2011) PMID 21922585

  5. Sonic Hedgehog-induced proliferation requires specific Gα inhibitory proteins.

    Journal of Biological Chemistry 286(10):8067 (2011) PMID 21209076 PMCID PMC3048693

    Proliferation of cerebellar granular neuronal precursors (CGNPs) is mediated by Sonic Hedgehog (Shh), which activates the Patched and Smoothened (Smo) receptor complex. Although its protein sequence suggests that Smo is a G protein coupled receptor (GPCR), the evidence that this receptor utilize...