1. An S-Acylation Switch of Conserved G Domain Cysteines Is Required for Polarity Signaling by ROP GTPases

    Current Biology 25(21):2875 (2015)

  2. Identification, Functional Characterization, and Evolution of Terpene Synthases from a Basal Dicot.

    Plant Physiology 169(3):1683 (2015) PMID 26157114 PMCID PMC4634067

    Bay laurel (Laurus nobilis) is an agriculturally and economically important dioecious tree in the basal dicot family Lauraceae used in food and drugs and in the cosmetics industry. Bay leaves, with their abundant monoterpenes and sesquiterpenes, are used to impart flavor and aroma to food, and h...
  3. Isolation and Functional Characterization of Carotenoid Cleavage Dioxygenase-1 from Laurus nobilis L. (Bay Laurel) Fruits.

    Journal of Agricultural and Food Chemistry 63(37):8275 (2015) PMID 26359684

    Bay laurel (Laurus nobilis L.) is an agriculturally important tree used in food, drugs, and the cosmetics industry. Many of the health beneficial properties of bay laurel are due to volatile terpene metabolites that they contain, including various norisoprenoids. Despite their importance, little...
  4. Enhanced formation of aromatic amino acids increases fragrance without affecting flower longevity or pigmentation in Petunia × hybrida.

    Plant Biotechnology Journal 13(1):125 (2015) PMID 25283446

    Purple Petunia × hybrida V26 plants accumulate fragrant benzenoid-phenylpropanoid molecules and anthocyanin pigments in their petals. These specialized metabolites are synthesized mainly from the aromatic amino acids phenylalanine. Here, we studied the profile of secondary metabolites of petunia...
  5. Systems approach for exploring the intricate associations between sweetness, color and aroma in melon fruits.

    BMC Plant Biology 15(1):71 (2015) PMID 25887588 PMCID PMC4448286

    Melon (Cucumis melo) fruits exhibit phenotypic diversity in several key quality determinants such as taste, color and aroma. Sucrose, carotenoids and volatiles are recognized as the key compounds shaping the above corresponding traits yet the full network of biochemical events underlying their s...
  6. The mono - and sesquiterpene content of aphid-induced galls on Pistacia palaestina is not a simple reflection of their composition in intact leaves.

    Journal of Chemical Ecology 40(6):632 (2014) PMID 24916768

    Pistacia palaestina Boiss. (Anacardiaceae), a sibling species of P. terebinthus also known as turpentine tree or terebinth tree, is common in the Levant region. The aphid Baizongia pistaciae L. manipulates the leaves of the plant to form large galls, which provide both food and protection for it...
  7. The PH gene determines fruit acidity and contributes to the evolution of sweet melons.

    Nature Communications 5:4026 (2014) PMID 24898284

    Taste has been the subject of human selection in the evolution of agricultural crops, and acidity is one of the three major components of fleshy fruit taste, together with sugars and volatile flavour compounds. We identify a family of plant-specific genes with a major effect on fruit acidity by ...
  8. Formation of norisoprenoid flavor compounds in carrot (Daucus carota L.) roots: characterization of a cyclic-specific carotenoid cleavage dioxygenase 1 gene.

    Journal of Agricultural and Food Chemistry 61(50):12244 (2013) PMID 24289159

    Carotenoids are isoprenoid pigments that upon oxidative cleavage lead to the production of norisoprenoids that have profound effect on flavor and aromas of agricultural products. The biosynthetic pathway to norisoprenoids in carrots (Daucus carota L.) is still largely unknown. We found the volat...
  9. Genetic and chemical characterization of an EMS induced mutation inCucumis meloCRTISO gene

    Archives of Biochemistry and Biophysics 539(2):117 (2013) PMID 23973661

    In order to broaden the available genetic variation of melon, we developed an ethyl methanesulfonate mutation library in an orange-flesh 'Charentais' type melon line that accumulates β-carotene. One mutagenized M2 family segregated for a novel recessive trait, a yellow-orange fruit flesh ('yofI'...
  10. Catabolism of L-methionine in the formation of sulfur and other volatiles in melon (Cucumis melo L.) fruit.

    Plant Journal 74(3):458 (2013) PMID 23402686

    Sulfur-containing aroma volatiles are important contributors to the distinctive aroma of melon and other fruits. Melon cultivars and accessions differ in the content of sulfur-containing and other volatiles. L-methionine has been postulated to serve as a precursor of these volatiles. Incubation ...
  11. Induced mutation in β-CAROTENE HYDROXYLASE results in accumulation of β-carotene and conversion of red to orange color in pepper fruit.

    Theoretical and Applied Genetics 126(3):557 (2013) PMID 23124390

    Pepper fruit is typically red, but green, orange and yellow cultivars are gaining consumer acceptance. This color variation is mainly due to variations in carotenoid composition. Orange color in pepper can result from a number of carotenoid profiles, but its genetic basis is only partly known. W...
  12. Benzaldehyde is a precursor of phenylpropylamino alkaloids as revealed by targeted metabolic profiling and comparative biochemical analyses in Ephedra spp.

    Phytochemistry 81:71 (2012) PMID 22727117

    Ephedrine and pseudoephedrine are phenylpropylamino alkaloids widely used in modern medicine. Some Ephedra species such as E. sinica Stapf (Ephedraceae), a widely used Chinese medicinal plant (Chinese name: Ma Huang), accumulate ephedrine alkaloids as active constituents. Other Ephedra species, ...
  13. Naturally occurring norephedrine oxazolidine derivatives in khat (Catha edulis).

    Planta Medica 78(8):838 (2012) PMID 22495440

    Khat (Catha edulis Forsk.) is a perennial shrub whose young leaves are chewed for their psychostimulating and anorectic properties. The main active principles of khat are believed to be the phenylpropylamino alkaloids, primarily (-)-cathinone [(S)-α-aminopropiophenone], (+)-cathine [(1S)(2S)-nor...
  14. Distribution of primary and specialized metabolites in Nigella sativa seeds, a spice with vast traditional and historical uses.

    Molecules 17(9):10159 (2012) PMID 22922285

    Black cumin (Nigella sativa L., Ranunculaceae) is an annual herb commonly used in the Middle East, India and nowadays gaining worldwide acceptance. Historical and traditional uses are extensively documented in ancient texts and historical documents. Black cumin seeds and oil are commonly used as...
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  15. Differential effects of prenylation and s-acylation on type I and II ROPS membrane interaction and function.

    Plant Physiology 155(2):706 (2011) PMID 21139084 PMCID PMC3032461

    Prenylation primarily by geranylgeranylation is required for membrane attachment and function of type I Rho of Plants (ROPs) and Gγ proteins, while type II ROPs are attached to the plasma membrane by S-acylation. Yet, it is not known how prenylation affects ROP membrane interaction dynamics and ...
  16. Study of 'Redhaven' peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism.

    BMC Plant Biology 11:24 (2011) PMID 21269483 PMCID PMC3045293

    Carotenoids are plant metabolites which are not only essential in photosynthesis but also important quality factors in determining the pigmentation and aroma of flowers and fruits. To investigate the regulation of carotenoid metabolism, as related to norisoprenoids and other volatile compounds i...
  17. Predator-released hydrocarbons repel oviposition by a mosquito.

    Ecology Letters 13(9):1129 (2010) PMID 20618841

    Prey species commonly use predator-released kairomones (PRKs) to detect risk of predation, yet the chemical identity of PRKs remains elusive. Chemical identification of PRKs will facilitate the study of predator-prey interactions and the risk of predation, and when the prey are pests, will poten...
  18. An S-acylation switch of conserved G domain cysteines is required for polarity signaling by ROP GTPases.

    Current Biology 20(10):914 (2010) PMID 20451389

    Rho GTPases are master regulators of cell polarity. For their function, Rhos must associate with discrete plasma membrane domains. Rho of Plants (ROPs) or RACs comprise a single family. Prenylation and S-acylation of hypervariable domain cysteines of Ras and Rho GTPases are required for their fu...
  19. Effects of wax coatings and postharvest storage on sensory quality and aroma volatile composition of 'Mor' mandarins.

    Journal of the Science of Food and Agriculture 90(6):995 (2010) PMID 20355140

    Mandarins suffer from accumulation of off-flavours after harvest. In this study the sensory quality and aroma profile composition of homogenised segments of untreated (control) and wax-coated 'Mor' mandarins after 7 days at 20 degrees C or 3 or 6 weeks of cold storage at 5 degrees C were examine...
  20. Metabolic networking in Brunfelsia calycina petals after flower opening.

    Journal of Experimental Botany 61(5):1393 (2010) PMID 20202996 PMCID PMC2837258

    Brunfelsia calycina flowers change colour from purple to white due to anthocyanin degradation, parallel to an increase in fragrance and petal size. Here it was tested whether the production of the fragrant benzenoids is dependent on induction of the shikimate pathway, or if they are formed from ...