1. Effects of fasting on serial measurements of hyperpolarized [1-(13) C]pyruvate metabolism in tumors.

    NMR in Biomedicine 29(8):1048 (2016) PMID 27309986 PMCID PMC4973679

    Imaging of the metabolism of hyperpolarized [1-(13) C]pyruvate has shown considerable promise in preclinical studies in oncology, particularly for the assessment of early treatment response. The repeatability of measurements of (13) C label exchange between pyruvate and lactate was determined in...
  2. A comparison of quantitative methods for clinical imaging with hyperpolarized (13)C-pyruvate.

    NMR in Biomedicine 29(4):387 (2016) PMID 27414749 PMCID PMC4833181

    Dissolution dynamic nuclear polarization (DNP) enables the metabolism of hyperpolarized (13)C-labelled molecules, such as the conversion of [1-(13)C]pyruvate to [1-(13)C]lactate, to be dynamically and non-invasively imaged in tissue. Imaging of this exchange reaction in animal models has been sh...
  3. Targeted transperineal biopsy of the prostate has limited additional benefit over background cores for larger MRI-identified tumors.

    World Journal of Urology 34(4):501 (2016) PMID 26238348 PMCID PMC4799791

    To compare histological outcomes in patients undergoing MRI-transrectal ultrasound fusion transperineal (MTTP) prostate biopsy and determine the incremental benefit of targeted cores. Seventy-six consecutive patients with 89 MRI-identified targets underwent MTTP biopsy. Separate targeted biopsie...
  4. MRI with hyperpolarised [1-13C]pyruvate detects advanced pancreatic preneoplasia prior to invasive disease in a mouse model.

    Gut 65(3):465 (2016) PMID 26347531 PMCID PMC4789827

    Pancreatic cancer (PCa) is treatable by surgery when detected at an early stage. Non-invasive imaging methods able to detect both established tumours and their precursor lesions are needed to select patients for surgery. We investigated here whether pancreatic preneoplasia could be detected prio...
  5. Defining the learning curve for multiparametric magnetic resonance imaging (MRI) of the prostate using MRI-transrectal ultrasonography (TRUS) fusion-guided transperineal prostate biopsies as a validation tool.

    BJU International 117(1):80 (2016) PMID 25099182

    To determine the accuracy of multiparametric magnetic resonance imaging (mpMRI) during the learning curve of radiologists using MRI targeted, transrectal ultrasonography (TRUS) guided transperineal fusion biopsy (MTTP) for validation. Prospective data on 340 men who underwent mpMRI (T2-weighted ...
  6. Evaluating Prostate Cancer Using Fractional Tissue Composition of Radical Prostatectomy Specimens and Pre-Operative Diffusional Kurtosis Magnetic Resonance Imaging.

    PLoS ONE 11(7):e0159652 (2016) PMID 27467064

    Evaluating tissue heterogeneity using non-invasive imaging could potentially improve prostate cancer assessment and treatment. 20 patients with intermediate/high-risk prostate cancer underwent diffusion kurtosis imaging, including calculation of apparent diffusion (Dapp) and kurtosis (Kapp), pri...
  7. Ratio of Tumor to Normal Prostate Tissue Apparent Diffusion Coefficient as a Method for Quantifying DWI of the Prostate.

    American Journal of Roentgenology 205(6):W585 (2015) PMID 26587948

    The purpose of this study was to investigate the ability of the apparent diffusion coefficient (ADC) ratio of tumor to normal prostate tissue to overcome inherent variability based on choice of b values, with whole-mount histopathologic analysis as the reference standard for tumor identification...
  8. Carbonic Anhydrase Activity Monitored In Vivo by Hyperpolarized 13C-Magnetic Resonance Spectroscopy Demonstrates Its Importance for pH Regulation in Tumors.

    Cancer Research 75(19):4109 (2015) PMID 26249175 PMCID PMC4594768

    Carbonic anhydrase buffers tissue pH by catalyzing the rapid interconversion of carbon dioxide (CO2) and bicarbonate (HCO3 (-)). We assessed the functional activity of CAIX in two colorectal tumor models, expressing different levels of the enzyme, by measuring the rate of exchange of hyperpolari...
  9. Identifying active vascular microcalcification by (18)F-sodium fluoride positron emission tomography.

    Nature Communications 6:7495 (2015) PMID 26151378 PMCID PMC4506997

    Vascular calcification is a complex biological process that is a hallmark of atherosclerosis. While macrocalcification confers plaque stability, microcalcification is a key feature of high-risk atheroma and is associated with increased morbidity and mortality. Positron emission tomography and X-...
  10. Investigating the ability of multiparametric MRI to exclude significant prostate cancer prior to transperineal biopsy.

    Canadian Urological Association Journal 9(11-12):E853 (2015) PMID 26788234 PMCID PMC4707904

    We characterized false negative prostate magnetic resonance imaging (MRI) reporting by using histology derived from MRI-transrectal ultrasound (TRUS)-guided transperineal (MTTP) fusion biopsies. In total, 148 consecutive patients were retrospectively reviewed. Men underwent multiparametric MRI (...
  11. Preoperative 3-T diffusion-weighted MRI for the qualitative and quantitative assessment of extracapsular extension in patients with intermediate- or high-risk prostate cancer.

    American Journal of Roentgenology 203(3):W280 (2014) PMID 25148185

    The purpose of this study was to prospectively evaluate the value of diffusion-weighted MRI (DWI) for the assessment of extracapsular extension (ECE) in patients with prostate cancer. Between November 2010 and April 2012, 40 patients with intermediate- to high-risk prostate cancer were prospecti...
  12. Prostate cancer: performance characteristics of combined T₂W and DW-MRI scoring in the setting of template transperineal re-biopsy using MR-TRUS fusion.

    European Radiology 24(7):1497 (2014) PMID 24744197

    To measure the performance characteristics of combined T2-weighted (T₂W) and diffusion-weighted (DW) magnetic resonance imaging (MRI) suspicion scoring prior to MR-transrectal ultrasound (TRUS) fusion template transperineal (TTP) re-biopsy. Thirty-nine patients referred for prostate re-biopsy, w...
  13. Magnetic resonance imaging with hyperpolarized [1,4-(13)C2]fumarate allows detection of early renal acute tubular necrosis.

    PNAS 109(33):13374 (2012) PMID 22837393 PMCID PMC3421192

    Acute kidney injury (AKI) is a common and important medical problem, affecting 10% of hospitalized patients, and it is associated with significant morbidity and mortality. The most frequent cause of AKI is acute tubular necrosis (ATN). Current imaging techniques and biomarkers do not allow ATN t...
  14. Disruption of mouse Cenpj, a regulator of centriole biogenesis, phenocopies Seckel syndrome.

    PLoS Genetics 8(11):e1003022 (2012) PMID 23166506 PMCID PMC3499256

    Disruption of the centromere protein J gene, CENPJ (CPAP, MCPH6, SCKL4), which is a highly conserved and ubiquitiously expressed centrosomal protein, has been associated with primary microcephaly and the microcephalic primordial dwarfism disorder Seckel syndrome. The mechanism by which disruptio...
  15. Imaging pH with hyperpolarized 13C.

    NMR in Biomedicine 24(8):1006 (2011) PMID 21812047

    pH is a fundamental physiological parameter that is tightly controlled by endogenous buffers. The acid-base balance is altered in many disease states, such as inflammation, ischemia and cancer. Despite the importance of pH, there are currently no routine methods for imaging the spatial distribut...
  16. Hyperpolarized 13C MRI and PET: in vivo tumor biochemistry.

    Journal of nuclear medicine : official publicat... 52(9):1333 (2011) PMID 21849405

    Dynamic nuclear polarization (DNP) is an emerging technique for dramatically increasing the sensitivity of magnetic resonance spectroscopy (MRS). This review evaluates the potential strengths and weaknesses of DNP-enhanced (13)C magnetic resonance spectroscopic imaging (DNP-MRSI) as a clinical i...
  17. Hyperpolarized [1-13C]-ascorbic and dehydroascorbic acid: vitamin C as a probe for imaging redox status in vivo.

    Journal of the American Chemical Society 133(30):11795 (2011) PMID 21692446 PMCID PMC3144679

    Dynamic nuclear polarization (DNP) of (13)C-labeled metabolic substrates in vitro and their subsequent intravenous administration allow both the location of the hyperpolarized substrate and the dynamics of its subsequent conversion into other metabolic products to be detected in vivo. We report ...
  18. Tumor imaging using hyperpolarized 13C magnetic resonance spectroscopy.

    Magnetic Resonance in Medicine 66(2):505 (2011) PMID 21661043

    Dynamic nuclear polarization is an emerging technique for increasing the sensitivity of magnetic resonance imaging and spectroscopy, particularly for low-γ nuclei. The technique has been applied recently to a number of 13C-labeled cell metabolites in biological systems: the increase in signal-to...
  19. Detection of tumor glutamate metabolism in vivo using (13)C magnetic resonance spectroscopy and hyperpolarized [1-(13)C]glutamate.

    Magnetic Resonance in Medicine 66(1):18 (2011) PMID 21695718

    Dynamic nuclear polarization can be used to increase the sensitivity of solution state (13)C magnetic resonance spectroscopy by four orders of magnitude. We show here that [1-(13)C]glutamate can be polarized to 28%, representing a 35,000-fold increase in its sensitivity to detection at 9.4 T and...
  20. Functional and molecular imaging with MRI: potential applications in paediatric radiology.

    Pediatric Radiology 41(2):185 (2011) PMID 20972674

    MRI is a very versatile tool for noninvasive imaging and it is particularly attractive as an imaging technique in paediatric patients given the absence of ionizing radiation. Recent advances in the field of MRI have enabled tissue function to be probed noninvasively, and increasingly MRI is bein...