1. Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver.

    Planta 236(5):1405 (2012) PMID 22729820

    Eucommia ulmoides Oliver is one of a few woody plants capable of producing abundant quantities of trans-polyisoprene rubber in their leaves, barks, and seed coats. One cDNA library each was constructed from its outer stem tissue and inner stem tissue. They comprised a total of 27,752 expressed s...
  2. Transgenic tobacco plants producing caffeine: a potential new strategy for insect pest control.

    Transgenic Research 15(6):667 (2006) PMID 17091387

    Caffeine (1,3,7-trimethylxanthine) is one of the most widely used plant secondary metabolites, primarily as a stimulant and an ingredient in drugs. In nature, caffeine is believed to function in chemical defense, acting as an antiherbivory and allelopathic agent, and therefore it might be employ...
  3. Caffeine production in tobacco plants by simultaneous expression of three coffee N-methyltrasferases and its potential as a pest repellant.

    Plant Molecular Biology 59(2):221 (2005) PMID 16247553

    Caffeine (1,3,7-trimethylxanthine) is derived from xanthosine through three successive transfers of methyl groups and a single ribose removal in coffee plants. The methyl group transfer is catalyzed by N-zmethyltransferases, xanthosine methyltransferase (XMT), 7-methylxanthine methyltransferase ...
  4. Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties.

    Plant Molecular Biology 54(6):931 (2004) PMID 15604660

    The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We ...
  5. Producing decaffeinated coffee plants.

    Nature 423(6942):823 (2003) PMID 12815419

  6. Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants.

    Plant Physiology 132(1):372 (2003) PMID 12746542 PMCID PMC166982

    Caffeine is synthesized from xanthosine through N-methylation and ribose removal steps. In the present study, three types of cDNAs encoding N-methyltransferases were isolated from immature fruits of coffee (Coffea arabica) plants, and designated as CaXMT1, CaMXMT2, and CaDXMT1, respectively. The...
  7. Bleaching of the Red Anthocyanin Induced by Superoxide Radical

    Archives of Biochemistry and Biophysics 332(1):183 (1996)

    Red anthocyanin prepared from petals of Hibiscus rosa-sinensisL. was photobleached in the EDTA–riboflavin system. The rate of bleaching monitored at 565 nm depended on the light intensity and EDTA concentrations. Anaerobic conditions and/or addition of superoxide dismutase prevented t...