1. Thermal proteome profiling monitors ligand interactions with cellular membrane proteins.

    Nature Methods 12(12):1129 (2015) PMID 26524241

    We extended thermal proteome profiling to detect transmembrane protein-small molecule interactions in cultured human cells. When we assessed the effects of detergents on ATP-binding profiles, we observed shifts in denaturation temperature for ATP-binding transmembrane proteins. We also observed ...
  2. Thermal proteome profiling for unbiased identification of direct and indirect drug targets using multiplexed quantitative mass spectrometry.

    Nature Protocols 10(10):1567 (2015) PMID 26379230

    The direct detection of drug-protein interactions in living cells is a major challenge in drug discovery research. Recently, we introduced an approach termed thermal proteome profiling (TPP), which enables the monitoring of changes in protein thermal stability across the proteome using quantitat...
  3. Automated label-free quantification of metabolites from liquid chromatography-mass spectrometry data.

    Molecular & Cellular Proteomics 13(1):348 (2014) PMID 24176773 PMCID PMC3879626

    Liquid chromatography coupled to mass spectrometry (LC-MS) has become a standard technology in metabolomics. In particular, label-free quantification based on LC-MS is easily amenable to large-scale studies and thus well suited to clinical metabolomics. Large-scale studies, however, require auto...
  4. Circulating lysophosphatidylcholines are markers of a metabolically benign nonalcoholic fatty liver.

    Diabetes Care 36(8):2331 (2013) PMID 23514731 PMCID PMC3714475

    Nonalcoholic fatty liver (NAFL) is thought to contribute to insulin resistance and its metabolic complications. However, some individuals with NAFL remain insulin sensitive. Mechanisms involved in the susceptibility to develop insulin resistance in humans with NAFL are largely unknown. We invest...
  5. Preanalytical aspects and sample quality assessment in metabolomics studies of human blood.

    Clinical Chemistry 59(5):833 (2013) PMID 23386698

    Metabolomics is a powerful tool that is increasingly used in clinical research. Although excellent sample quality is essential, it can easily be compromised by undetected preanalytical errors. We set out to identify critical preanalytical steps and biomarkers that reflect preanalytical inaccurac...