1. Chromosome Segregation: A Spatial Code to Correct Kinetochore-Microtubule Attachments.

    Current Biology 25(14):R601 (2015) PMID 26196485

    Erroneous kinetochore-microtubule interactions must be detected and corrected before a cell enters anaphase to prevent chromosome mis-segregation. Two new studies describe an Aurora A-mediated error correction mechanism based on the spatial position of a chromosome within the mitotic spindle. C...
  2. Distinct Organization and Regulation of the Outer Kinetochore KMN Network Downstream of CENP-C and CENP-T.

    Current Biology 25(5):671 (2015) PMID 25660545 PMCID PMC4348146

    The kinetochore provides a vital connection between chromosomes and spindle microtubules [1, 2]. Defining the molecular architecture of the core kinetochore components is critical for understanding the mechanisms by which the kinetochore directs chromosome segregation. The KNL1/Mis12 complex/Ndc...
  3. The outer kinetochore protein KNL-1 contains a defined oligomerization domain in nematodes.

    Molecular Biology of the Cell 26(2):229 (2015) PMID 25411336 PMCID PMC4294671

    The kinetochore is a large, macromolecular assembly that is essential for connecting chromosomes to microtubules during mitosis. Despite the recent identification of multiple kinetochore components, the nature and organization of the higher-order kinetochore structure remain unknown. The outer k...
  4. The outer kinetochore protein KNL-1 contains a defined oligomerization domain in nematodes.

    Molecular Biology of the Cell 26(2):229 (2015) PMID 25411336 PMCID PMC4294671

    The kinetochore is a large, macromolecular assembly that is essential for connecting chromosomes to microtubules during mitosis. Despite the recent identification of multiple kinetochore components, the nature and organization of the higher-order kinetochore structure remain unknown. The outer k...
  5. Resonance assignments of the microtubule-binding domain of the C. elegans spindle and kinetochore-associated protein 1.

    Biomolecular NMR Assignments 8(2):275 (2014) PMID 23765286 PMCID PMC3823668

    During mitosis, kinetochores coordinate the attachment of centromeric DNA to the dynamic plus ends of microtubules, which is hypothesized to pull sister chromatids toward opposing poles of the mitotic spindle. The outer kinetochore Ndc80 complex acts synergistically with the Ska (spindle and kin...
  6. Resonance assignments of the microtubule-binding domain of the C. elegans spindle and kinetochore-associated protein 1.

    Biomolecular NMR Assignments 8(2):275 (2014) PMID 23765286 PMCID PMC3823668

    During mitosis, kinetochores coordinate the attachment of centromeric DNA to the dynamic plus ends of microtubules, which is hypothesized to pull sister chromatids toward opposing poles of the mitotic spindle. The outer kinetochore Ndc80 complex acts synergistically with the Ska (spindle and kin...
  7. Resonance assignments of the microtubule-binding domain of the C. elegans spindle and kinetochore-associated protein 1.

    Biomolecular NMR Assignments 8(2):275 (2014) PMID 23765286 PMCID PMC3823668

    During mitosis, kinetochores coordinate the attachment of centromeric DNA to the dynamic plus ends of microtubules, which is hypothesized to pull sister chromatids toward opposing poles of the mitotic spindle. The outer kinetochore Ndc80 complex acts synergistically with the Ska (spindle and kin...
  8. Polo-like kinase 1 licenses CENP-A deposition at centromeres.

    Cell 158(2):397 (2014) PMID 25036634 PMCID PMC4192726

    To ensure the stable transmission of the genome during vertebrate cell division, the mitotic spindle must attach to a single locus on each chromosome, termed the centromere. The fundamental requirement for faithful centromere inheritance is the controlled deposition of the centromere-specifying ...
  9. Polo-like kinase 1 licenses CENP-A deposition at centromeres.

    Cell 158(2):397 (2014) PMID 25036634 PMCID PMC4192726

    To ensure the stable transmission of the genome during vertebrate cell division, the mitotic spindle must attach to a single locus on each chromosome, termed the centromere. The fundamental requirement for faithful centromere inheritance is the controlled deposition of the centromere-specifying ...
  10. The kinetochore.

    Cold Spring Harbor Perspectives in Biology 6(7):a015826 (2014) PMID 24984773

    A critical requirement for mitosis is the distribution of genetic material to the two daughter cells. The central player in this process is the macromolecular kinetochore structure, which binds to both chromosomal DNA and spindle microtubule polymers to direct chromosome alignment and segregatio...
  11. Kinetochore genes are coordinately up-regulated in human tumors as part of a FoxM1-related cell division program.

    Molecular Biology of the Cell 25(13):1983 (2014) PMID 24829384 PMCID PMC4072572

    The key player in directing proper chromosome segregation is the macromolecular kinetochore complex, which mediates DNA-microtubule interactions. Previous studies testing individual kinetochore genes documented examples of their overexpression in tumors relative to normal tissue, leading to prop...
  12. Kinetochore genes are coordinately up-regulated in human tumors as part of a FoxM1-related cell division program.

    Molecular Biology of the Cell 25(13):1983 (2014) PMID 24829384 PMCID PMC4072572

    The key player in directing proper chromosome segregation is the macromolecular kinetochore complex, which mediates DNA-microtubule interactions. Previous studies testing individual kinetochore genes documented examples of their overexpression in tumors relative to normal tissue, leading to prop...
  13. Kinetochore genes are coordinately up-regulated in human tumors as part of a FoxM1-related cell division program.

    Molecular Biology of the Cell 25(13):1983 (2014) PMID 24829384 PMCID PMC4072572

    The key player in directing proper chromosome segregation is the macromolecular kinetochore complex, which mediates DNA-microtubule interactions. Previous studies testing individual kinetochore genes documented examples of their overexpression in tumors relative to normal tissue, leading to prop...
  14. The kinetochore.

    Cold Spring Harbor Perspectives in Biology 6(7):a015826 (2014) PMID 24984773

    A critical requirement for mitosis is the distribution of genetic material to the two daughter cells. The central player in this process is the macromolecular kinetochore structure, which binds to both chromosomal DNA and spindle microtubule polymers to direct chromosome alignment and segregatio...
  15. Spindle assembly checkpoint robustness requires Tpr-mediated regulation of Mad1/Mad2 proteostasis.

    Journal of Cell Biology 203(6):883 (2013) PMID 24344181 PMCID PMC3871433

    Tpr is a conserved nuclear pore complex (NPC) protein implicated in the spindle assembly checkpoint (SAC) by an unknown mechanism. Here, we show that Tpr is required for normal SAC response by stabilizing Mad1 and Mad2 before mitosis. Tpr coimmunoprecipitated with Mad1 and Mad2 (hereafter design...
  16. Spindle assembly checkpoint robustness requires Tpr-mediated regulation of Mad1/Mad2 proteostasis.

    Journal of Cell Biology 203(6):883 (2013) PMID 24344181 PMCID PMC3871433

    Tpr is a conserved nuclear pore complex (NPC) protein implicated in the spindle assembly checkpoint (SAC) by an unknown mechanism. Here, we show that Tpr is required for normal SAC response by stabilizing Mad1 and Mad2 before mitosis. Tpr coimmunoprecipitated with Mad1 and Mad2 (hereafter design...
  17. Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in Anaphase

    Cell 154(6):1401 (2013)

  18. Cortical Dynein and Asymmetric Membrane Elongation Coordinately Position the Spindle in Anaphase

    Cell 154(2):391 (2013)

    Mitotic spindle position defines the cell-cleavage site during cytokinesis. However, the mechanisms that control spindle positioning to generate equal-sized daughter cells remain poorly understood. Here, we demonstrate that two mechanisms act coordinately to center the spindle during a...
  19. Cortical dynein and asymmetric membrane elongation coordinately position the spindle in anaphase.

    Cell 154(2):391 (2013) PMID 23870127 PMCID PMC4177044

    Mitotic spindle position defines the cell-cleavage site during cytokinesis. However, the mechanisms that control spindle positioning to generate equal-sized daughter cells remain poorly understood. Here, we demonstrate that two mechanisms act coordinately to center the spindle during anaphase in...
  20. Induced dicentric chromosome formation promotes genomic rearrangements and tumorigenesis.

    Chromosome Research 21(4):407 (2013) PMID 23793898 PMCID PMC3713265

    Chromosomal rearrangements can radically alter gene products and their function, driving tumor formation or progression. However, the molecular origins and evolution of such rearrangements are varied and poorly understood, with cancer cells often containing multiple, complex rearrangements. One ...