1. Development of connectivity in a motoneuronal network in Drosophila larvae.

    Current Biology 25(5):568 (2015) PMID 25702582

    Much of our understanding of how neural networks develop is based on studies of sensory systems, revealing often highly stereotyped patterns of connections, particularly as these diverge from the presynaptic terminals of sensory neurons. We know considerably less about the wiring strategies of m...
  2. Fast imaging of live organisms with sculpted light sheets.

    Scientific reports 5:9385 (2015) PMID 25893952 PMCID PMC4403519

    Light-sheet microscopy is an increasingly popular technique in the life sciences due to its fast 3D imaging capability of fluorescent samples with low photo toxicity compared to confocal methods. In this work we present a new, fast, flexible and simple to implement method to optimize the illumin...
  3. Development of connectivity in a motoneuronal network in Drosophila larvae

    Current Biology (2014)

    Background: Much of our understanding of how neural networks develop is based on studies of sensory systems, revealing often highly stereotyped patterns of connections, particularly as these diverge from the presynaptic terminals of sensory neurons. We know considerably less about the ...
  4. Dendritic growth gated by a steroid hormone receptor underlies increases in activity in the developing Drosophila locomotor system.

    PNAS 110(40):E3878 (2013) PMID 24043825 PMCID PMC3791713

    As animals grow, their nervous systems also increase in size. How growth in the central nervous system is regulated and its functional consequences are incompletely understood. We explored these questions, using the larval Drosophila locomotor system as a model. In the periphery, at neuromuscula...
  5. Dendritic growth gated by a steroid hormone receptor underlies increases in activity in the developing Drosophila locomotor system.

    PNAS 110(40):E3878 (2013) PMID 24043825 PMCID PMC3791713

    As animals grow, their nervous systems also increase in size. How growth in the central nervous system is regulated and its functional consequences are incompletely understood. We explored these questions, using the larval Drosophila locomotor system as a model. In the periphery, at neuromuscula...
  6. Sensory neuron-derived eph regulates glomerular arbors and modulatory function of a central serotonergic neuron.

    PLoS Genetics 9(4):e1003452 (2013) PMID 23637622 PMCID PMC3630106

    Olfactory sensory neurons connect to the antennal lobe of the fly to create the primary units for processing odor cues, the glomeruli. Unique amongst antennal-lobe neurons is an identified wide-field serotonergic neuron, the contralaterally-projecting, serotonin-immunoreactive deutocerebral neur...
  7. Sensory neuron-derived eph regulates glomerular arbors and modulatory function of a central serotonergic neuron.

    PLoS Genetics 9(4):e1003452 (2013) PMID 23637622 PMCID PMC3630106

    Olfactory sensory neurons connect to the antennal lobe of the fly to create the primary units for processing odor cues, the glomeruli. Unique amongst antennal-lobe neurons is an identified wide-field serotonergic neuron, the contralaterally-projecting, serotonin-immunoreactive deutocerebral neur...
  8. Neural circuits for peristaltic wave propagation in crawling Drosophila larvae: analysis and modeling.

    Frontiers in Computational Neuroscience 7:24 (2013) PMID 23576980 PMCID PMC3616270

    Drosophila larvae crawl by peristaltic waves of muscle contractions, which propagate along the animal body and involve the simultaneous contraction of the left and right side of each segment. Coordinated propagation of contraction does not require sensory input, suggesting that movement is gener...
  9. Neural circuits for peristaltic wave propagation in crawling Drosophila larvae: analysis and modeling.

    Frontiers in Computational Neuroscience 7:24 (2013) PMID 23576980 PMCID PMC3616270

    Drosophila larvae crawl by peristaltic waves of muscle contractions, which propagate along the animal body and involve the simultaneous contraction of the left and right side of each segment. Coordinated propagation of contraction does not require sensory input, suggesting that movement is gener...
  10. Ovine PrP transgenic Drosophila show reduced locomotor activity and decreased survival.

    Biochemical Journal 444(3):487 (2012) PMID 22435640

    Drosophila have emerged as a model system to study mammalian neurodegenerative diseases. In the present study we have generated Drosophila transgenic for ovine PrP (prion protein) to begin to establish an invertebrate model of ovine prion disease. We generated Drosophila transgenic for polymorph...
  11. Ovine PrP transgenic Drosophila show reduced locomotor activity and decreased survival.

    Biochemical Journal 444(3):487 (2012) PMID 22435640

    Drosophila have emerged as a model system to study mammalian neurodegenerative diseases. In the present study we have generated Drosophila transgenic for ovine PrP (prion protein) to begin to establish an invertebrate model of ovine prion disease. We generated Drosophila transgenic for polymorph...
  12. Endogenous patterns of activity are required for the maturation of a motor network.

    Journal of Neuroscience 31(29):10445 (2011) PMID 21775590 PMCID PMC3492740

    Many parts of the nervous system become active before development is complete, including the embryonic spinal cord. Remarkably, although the subject has been debated for over a century (Harrison, 1904), it is still unclear whether such activity is required for normal development of motor circuit...
  13. Endogenous patterns of activity are required for the maturation of a motor network.

    Journal of Neuroscience 31(29):10445 (2011) PMID 21775590 PMCID PMC3492740

    Many parts of the nervous system become active before development is complete, including the embryonic spinal cord. Remarkably, although the subject has been debated for over a century (Harrison, 1904), it is still unclear whether such activity is required for normal development of motor circuit...
  14. Midline signalling systems direct the formation of a neural map by dendritic targeting in the Drosophila motor system.

    PLoS Biology 7(9):e1000200 (2009) PMID 19771146 PMCID PMC2736389

    A fundamental strategy for organising connections in the nervous system is the formation of neural maps. Map formation has been most intensively studied in sensory systems where the central arrangement of axon terminals reflects the distribution of sensory neuron cell bodies in the periphery or ...
  15. Midline signalling systems direct the formation of a neural map by dendritic targeting in the Drosophila motor system.

    PLoS Biology 7(9):e1000200 (2009) PMID 19771146 PMCID PMC2736389

    A fundamental strategy for organising connections in the nervous system is the formation of neural maps. Map formation has been most intensively studied in sensory systems where the central arrangement of axon terminals reflects the distribution of sensory neuron cell bodies in the periphery or ...
  16. Developmental changes in dendritic shape and synapse location tune single-neuron computations to changing behavioral functions.

    Journal of Neurophysiology 102(1):41 (2009) PMID 19386754

    During nervous system development, different classes of neurons obtain different dendritic architectures, each of which receives a large number of input synapses. However, it is not clear whether synaptic inputs are targeted to specific regions within a dendritic tree and whether dendritic tree ...
  17. Developmental changes in dendritic shape and synapse location tune single-neuron computations to changing behavioral functions.

    Journal of Neurophysiology 102(1):41 (2009) PMID 19386754

    During nervous system development, different classes of neurons obtain different dendritic architectures, each of which receives a large number of input synapses. However, it is not clear whether synaptic inputs are targeted to specific regions within a dendritic tree and whether dendritic tree ...
  18. PTX-induced hyperexcitability affects dendritic shape and GABAergic synapse density but not synapse distribution during Manduca postembryonic motoneuron development.

    Journal of Comparative Physiology, A 195(5):473 (2009) PMID 19252912

    During the metamorphosis of the holometabolous insect, Manduca sexta, the postembryonic acquisition of adult specific motor behaviors is accompanied by changes in dendritic architecture, membrane currents, and input synapses of identified motoneurons. This study aims to test whether increased ac...
  19. PTX-induced hyperexcitability affects dendritic shape and GABAergic synapse density but not synapse distribution during Manduca postembryonic motoneuron development.

    Journal of Comparative Physiology, A 195(5):473 (2009) PMID 19252912

    During the metamorphosis of the holometabolous insect, Manduca sexta, the postembryonic acquisition of adult specific motor behaviors is accompanied by changes in dendritic architecture, membrane currents, and input synapses of identified motoneurons. This study aims to test whether increased ac...
  20. The development of motor coordination in Drosophila embryos.

    Development 135(22):3707 (2008) PMID 18927150 PMCID PMC3501643

    We used non-invasive muscle imaging to study the onset of motor activity and emergence of coordinated movement in Drosophila embryos. Earliest movements are myogenic, and neurally controlled muscle contractions first appear with the onset of bursting activity 17 hours after egg laying. Initial e...