1. Early Telomerase Inactivation Accelerates Aging Independently of Telomere Length

    Cell 160(5):928 (2015)

    Telomerase is required for long-term telomere maintenance and protection. Using single budding yeast mother cell analyses we found that, even early after telomerase inactivation (ETI), yeast mother cells show transient DNA damage response (DDR) episodes, stochastically altered cell-cyc...
  2. Early telomerase inactivation accelerates aging independently of telomere length.

    Cell 160(5):928 (2015) PMID 25723167

    Telomerase is required for long-term telomere maintenance and protection. Using single budding yeast mother cell analyses we found that, even early after telomerase inactivation (ETI), yeast mother cells show transient DNA damage response (DDR) episodes, stochastically altered cell-cycle dynamic...
  3. Early telomerase inactivation accelerates aging independently of telomere length.

    Cell 160(5):928 (2015) PMID 25723167 PMCID PMC4496004

    Telomerase is required for long-term telomere maintenance and protection. Using single budding yeast mother cell analyses we found that, even early after telomerase inactivation (ETI), yeast mother cells show transient DNA damage response (DDR) episodes, stochastically altered cell-cycle dynamic...
  4. Heterogeneously expressed fezf2 patterns gradient Notch activity in balancing the quiescence, proliferation, and differentiation of adult neural stem cells.

    Journal of Neuroscience 34(42):13911 (2014) PMID 25319688 PMCID PMC4198537

    Balancing quiescence, self-renewal, and differentiation in adult stem cells is critical for tissue homeostasis. The underlying mechanisms, however, remain incompletely understood. Here we identify Fezf2 as a novel regulator of fate balance in adult zebrafish dorsal telencephalic neural stem cell...
  5. Heterogeneously expressed fezf2 patterns gradient Notch activity in balancing the quiescence, proliferation, and differentiation of adult neural stem cells.

    Journal of Neuroscience 34(42):13911 (2014) PMID 25319688 PMCID PMC4198537

    Balancing quiescence, self-renewal, and differentiation in adult stem cells is critical for tissue homeostasis. The underlying mechanisms, however, remain incompletely understood. Here we identify Fezf2 as a novel regulator of fate balance in adult zebrafish dorsal telencephalic neural stem cell...
  6. Heterogeneously expressed fezf2 patterns gradient Notch activity in balancing the quiescence, proliferation, and differentiation of adult neural stem cells.

    Journal of Neuroscience 34(42):13911 (2014) PMID 25319688 PMCID PMC4198537

    Balancing quiescence, self-renewal, and differentiation in adult stem cells is critical for tissue homeostasis. The underlying mechanisms, however, remain incompletely understood. Here we identify Fezf2 as a novel regulator of fate balance in adult zebrafish dorsal telencephalic neural stem cell...
  7. Heterogeneously expressed fezf2 patterns gradient Notch activity in balancing the quiescence, proliferation, and differentiation of adult neural stem cells.

    Journal of Neuroscience 34(42):13911 (2014) PMID 25319688 PMCID PMC4198537

    Balancing quiescence, self-renewal, and differentiation in adult stem cells is critical for tissue homeostasis. The underlying mechanisms, however, remain incompletely understood. Here we identify Fezf2 as a novel regulator of fate balance in adult zebrafish dorsal telencephalic neural stem cell...
  8. Early Telomerase Inactivation Accelerates Aging Independently of Telomere Length

    Cell (2014)

    Telomerase is required for long-term telomere maintenance and protection. Using single budding yeast mother cell analyses we found that, even Early after Telomerase Inactivation (ETI), yeast mother cells show transient DNA Damage Response (DDR) episodes, stochastically altered cell cyc...
  9. Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells.

    PLoS Genetics 9(9):e1003821 (2013) PMID 24086155 PMCID PMC3784563

    Argonaute proteins are often credited for their cytoplasmic activities in which they function as central mediators of the RNAi platform and microRNA (miRNA)-mediated processes. They also facilitate heterochromatin formation and establishment of repressive epigenetic marks in the nucleus of fissi...
  10. Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells.

    PLoS Genetics 9(9):e1003821 (2013) PMID 24086155 PMCID PMC3784563

    Argonaute proteins are often credited for their cytoplasmic activities in which they function as central mediators of the RNAi platform and microRNA (miRNA)-mediated processes. They also facilitate heterochromatin formation and establishment of repressive epigenetic marks in the nucleus of fissi...
  11. The unfolded protein response in fission yeast modulates stability of select mRNAs to maintain protein homeostasis.

    eLife 1:e00048 (2012) PMID 23066505 PMCID PMC3470409

    The unfolded protein response (UPR) monitors the protein folding capacity of the endoplasmic reticulum (ER). In all organisms analyzed to date, the UPR drives transcriptional programs that allow cells to cope with ER stress. The non-conventional splicing of Hac1 (yeasts) and XBP1 (metazoans) mRN...
  12. The unfolded protein response in fission yeast modulates stability of select mRNAs to maintain protein homeostasis.

    eLife 1:e00048 (2012) PMID 23066505 PMCID PMC3470409

    The unfolded protein response (UPR) monitors the protein folding capacity of the endoplasmic reticulum (ER). In all organisms analyzed to date, the UPR drives transcriptional programs that allow cells to cope with ER stress. The non-conventional splicing of Hac1 (yeasts) and XBP1 (metazoans) mRN...
  13. Genomic selection identifies vertebrate transcription factor Fezf2 binding sites and target genes.

    Journal of Biological Chemistry 286(21):18641 (2011) PMID 21471212 PMCID PMC3099680

    Identification of transcription factor targets is critical to understanding gene regulatory networks. Here, we uncover transcription factor binding sites and target genes employing systematic evolution of ligands by exponential enrichment (SELEX). Instead of selecting randomly synthesized DNA ol...
  14. Genomic selection identifies vertebrate transcription factor Fezf2 binding sites and target genes.

    Journal of Biological Chemistry 286(21):18641 (2011) PMID 21471212 PMCID PMC3099680

    Identification of transcription factor targets is critical to understanding gene regulatory networks. Here, we uncover transcription factor binding sites and target genes employing systematic evolution of ligands by exponential enrichment (SELEX). Instead of selecting randomly synthesized DNA ol...
  15. Epistatic relationships reveal the functional organization of yeast transcription factors.

    Molecular Systems Biology 6:420 (2010) PMID 20959818 PMCID PMC2990640

    The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site-specific DNA-binding transcription factors (STFs). Here, quantitative genetic profiling using the epistat...
  16. Epistatic relationships reveal the functional organization of yeast transcription factors.

    Molecular Systems Biology 6:420 (2010) PMID 20959818 PMCID PMC2990640

    The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site-specific DNA-binding transcription factors (STFs). Here, quantitative genetic profiling using the epistat...
  17. De novo identification and biophysical characterization of transcription-factor binding sites with microfluidic affinity analysis.

    Nature Biotechnology 28(9):970 (2010) PMID 20802496 PMCID PMC2937095

    Gene expression is regulated in part by protein transcription factors that bind target regulatory DNA sequences. Predicting DNA binding sites and affinities from transcription factor sequence or structure is difficult; therefore, experimental data are required to link transcription factors to ta...
  18. De novo identification and biophysical characterization of transcription-factor binding sites with microfluidic affinity analysis.

    Nature Biotechnology 28(9):970 (2010) PMID 20802496 PMCID PMC2937095

    Gene expression is regulated in part by protein transcription factors that bind target regulatory DNA sequences. Predicting DNA binding sites and affinities from transcription factor sequence or structure is difficult; therefore, experimental data are required to link transcription factors to ta...
  19. fREDUCE: detection of degenerate regulatory elements using correlation with expression.

    BMC Bioinformatics 8:399 (2007) PMID 17941998 PMCID PMC2174516

    The precision of transcriptional regulation is made possible by the specificity of physical interactions between transcription factors and their cognate binding sites on DNA. A major challenge is to decipher transcription factor binding sites from sequence and functional genomic data using compu...
  20. fREDUCE: detection of degenerate regulatory elements using correlation with expression.

    BMC Bioinformatics 8:399 (2007) PMID 17941998 PMCID PMC2174516

    The precision of transcriptional regulation is made possible by the specificity of physical interactions between transcription factors and their cognate binding sites on DNA. A major challenge is to decipher transcription factor binding sites from sequence and functional genomic data using compu...