1. Distinct Organization and Regulation of the Outer Kinetochore KMN Network Downstream of CENP-C and CENP-T.

    Current Biology 25(5):671 (2015) PMID 25660545 PMCID PMC4348146

    The kinetochore provides a vital connection between chromosomes and spindle microtubules [1, 2]. Defining the molecular architecture of the core kinetochore components is critical for understanding the mechanisms by which the kinetochore directs chromosome segregation. The KNL1/Mis12 complex/Ndc...
  2. Induced dicentric chromosome formation promotes genomic rearrangements and tumorigenesis.

    Chromosome Research 21(4):407 (2013) PMID 23793898 PMCID PMC3713265

    Chromosomal rearrangements can radically alter gene products and their function, driving tumor formation or progression. However, the molecular origins and evolution of such rearrangements are varied and poorly understood, with cancer cells often containing multiple, complex rearrangements. One ...
  3. Induced dicentric chromosome formation promotes genomic rearrangements and tumorigenesis.

    Chromosome Research 21(4):407 (2013) PMID 23793898 PMCID PMC3713265

    Chromosomal rearrangements can radically alter gene products and their function, driving tumor formation or progression. However, the molecular origins and evolution of such rearrangements are varied and poorly understood, with cancer cells often containing multiple, complex rearrangements. One ...
  4. CDK-dependent phosphorylation and nuclear exclusion coordinately control kinetochore assembly state.

    Journal of Cell Biology 201(1):23 (2013) PMID 23530067 PMCID PMC3613690

    Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex. Prior work has identified more than 100 different kinetochore components in human cells. However, little is known about the regulatory processes that specify their assembly upon mitotic entry and disassemb...
  5. CDK-dependent phosphorylation and nuclear exclusion coordinately control kinetochore assembly state.

    Journal of Cell Biology 201(1):23 (2013) PMID 23530067 PMCID PMC3613690

    Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex. Prior work has identified more than 100 different kinetochore components in human cells. However, little is known about the regulatory processes that specify their assembly upon mitotic entry and disassemb...
  6. T time for point centromeres.

    Nature Cell Biology 14(6):559 (2012) PMID 22561349

    The diverse nature of eukaryotic centromere structure has led to a prevailing view that the kinetochore-chromatin interface is fundamentally different in distinct species. Two studies now challenge this dogma with the identification of budding yeast homologues of the vertebrate centromere DNA-bi...
  7. T time for point centromeres.

    Nature Cell Biology 14(6):559 (2012) PMID 22561349

    The diverse nature of eukaryotic centromere structure has led to a prevailing view that the kinetochore-chromatin interface is fundamentally different in distinct species. Two studies now challenge this dogma with the identification of budding yeast homologues of the vertebrate centromere DNA-bi...
  8. CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold

    Cell 148(3):487 (2012)

    The multiprotein kinetochore complex must assemble at a specific site on each chromosome to achieve accurate chromosome segregation. Defining the nature of the DNA-protein interactions that specify the position of the kinetochore and provide a scaffold for kinetochore formation remain ...
  9. CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold.

    Cell 148(3):487 (2012) PMID 22304917 PMCID PMC3277711

    The multiprotein kinetochore complex must assemble at a specific site on each chromosome to achieve accurate chromosome segregation. Defining the nature of the DNA-protein interactions that specify the position of the kinetochore and provide a scaffold for kinetochore formation remain key goals....
  10. Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes.

    Cell 145(3):410 (2011) PMID 21529714 PMCID PMC3085131

    Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. Although prior work identified the centromeric histone H3-variant CENP-A as the important upstream factor necessary for centromere specification, in human cells CENP-A is not sufficient for ...
  11. Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes.

    Cell 145(3):410 (2011) PMID 21529714 PMCID PMC3085131

    Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. Although prior work identified the centromeric histone H3-variant CENP-A as the important upstream factor necessary for centromere specification, in human cells CENP-A is not sufficient for ...
  12. Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes.

    Cell 145(3):410 (2011) PMID 21529714 PMCID PMC3085131

    Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. Although prior work identified the centromeric histone H3-variant CENP-A as the important upstream factor necessary for centromere specification, in human cells CENP-A is not sufficient for ...
  13. Kinetochore assembly: if you build it, they will come.

    Current Opinion in Cell Biology 23(1):102 (2011) PMID 20702077 PMCID PMC2980799

    Accurate chromosome segregation requires the interaction of chromosomes with the microtubules from the mitotic spindle. This interaction is mediated by the macro-molecular kinetochore complex, which assembles only at the centromeric region of each chromosome. However, how this site is specified ...
  14. Kinetochore assembly: if you build it, they will come

    Current Opinion in Cell Biology 23(1):102 (2011) PMID 20702077 PMCID PMC2980799

    Accurate chromosome segregation requires the interaction of chromosomes with the microtubules from the mitotic spindle. This interaction is mediated by the macro-molecular kinetochore complex, which assembles only at the centromeric region of each chromosome. However, how this site is ...
  15. Kinetochore assembly: if you build it, they will come

    Current Opinion in Cell Biology 23(1):102 (2011) PMID 20702077 PMCID PMC2980799

    Accurate chromosome segregation requires the interaction of chromosomes with the microtubules from the mitotic spindle. This interaction is mediated by the macro-molecular kinetochore complex, which assembles only at the centromeric region of each chromosome. However, how this site is ...
  16. Induced Ectopic Kinetochore Assembly Bypasses the Requirement for CENP-A Nucleosomes

    Cell 145(3):410 (2011)

    Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. Although prior work identified the centromeric histone H3-variant CENP-A as the important upstream factor necessary for centromere specification, in human cells CENP-A is not sufficient...
  17. How do anti-mitotic drugs kill cancer cells?

    Journal of Cell Science 122(Pt 15):2579 (2009) PMID 19625502

    In 2007, over 12-million people were diagnosed with cancer. According to the American Cancer Society, at least one third of these individuals are not expected to survive the disease, making cancer the second most prevalent cause of death worldwide. Systemic chemotherapy forms the mainstay of can...
  18. How do anti-mitotic drugs kill cancer cells?

    Journal of Cell Science 122(Pt 15):2579 (2009) PMID 19625502

    In 2007, over 12-million people were diagnosed with cancer. According to the American Cancer Society, at least one third of these individuals are not expected to survive the disease, making cancer the second most prevalent cause of death worldwide. Systemic chemotherapy forms the mainstay of can...
  19. Cancer cells display profound intra- and interline variation following prolonged exposure to antimitotic drugs.

    Cancer Cell 14(2):111 (2008) PMID 18656424

    Drugs targeting the mitotic spindle are used extensively during chemotherapy, but surprisingly, little is known about how they kill tumor cells. This is largely because many of the population-based approaches are indirect and lead to vague and confusing interpretations. Here, we use a high-throu...
  20. Cancer cells display profound intra- and interline variation following prolonged exposure to antimitotic drugs.

    Cancer Cell 14(2):111 (2008) PMID 18656424

    Drugs targeting the mitotic spindle are used extensively during chemotherapy, but surprisingly, little is known about how they kill tumor cells. This is largely because many of the population-based approaches are indirect and lead to vague and confusing interpretations. Here, we use a high-throu...