1. Tetravalent dengue DIIIC protein together with alum and ODN elicits a Th1 response and neutralizing antibodies in mice

    Vaccine 33(12):1474 (2015)

    • Tetra DIIIC formulated with ODN and alum induces a Th1 response to DENV-1–4. • Long-lasting EDIII-specific antibodies are produced by long-lived plasma cells. ...
  2. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.

    PLoS Pathogens 10(12):e1004548 (2014) PMID 25474532 PMCID PMC4256468

    Dengue is a growing global concern with 390 million people infected each year. Dengue virus (DENV) is transmitted by mosquitoes, thus host cells in the skin are the first point of contact with the virus. Human skin contains several populations of antigen-presenting cells which could drive the im...
  3. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.

    PLoS Pathogens 10(12):e1004548 (2014) PMID 25474532 PMCID PMC4256468

    Dengue is a growing global concern with 390 million people infected each year. Dengue virus (DENV) is transmitted by mosquitoes, thus host cells in the skin are the first point of contact with the virus. Human skin contains several populations of antigen-presenting cells which could drive the im...
  4. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.

    PLoS Pathogens 10(12):e1004548 (2014) PMID 25474532

    Dengue is a growing global concern with 390 million people infected each year. Dengue virus (DENV) is transmitted by mosquitoes, thus host cells in the skin are the first point of contact with the virus. Human skin contains several populations of antigen-presenting cells which could drive the im...
  5. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.

    PLoS Pathogens 10(12):e1004548 (2014) PMID 25474532 PMCID PMC4256468

    Dengue is a growing global concern with 390 million people infected each year. Dengue virus (DENV) is transmitted by mosquitoes, thus host cells in the skin are the first point of contact with the virus. Human skin contains several populations of antigen-presenting cells which could drive the im...
  6. Kinome siRNA screen identifies novel cell-type specific dengue host target genes

    Antiviral Research 110:20 (2014)

    • We developed a DENV-2 siRNA screen using a human kinase library in Huh7 cells. • Expression of validated targets were analysed in human Huh7 and U937 cells. ...
  7. Kinome siRNA screen identifies novel cell-type specific dengue host target genes.

    Antiviral Research 110:20 (2014) PMID 25046486

    Dengue is a global emerging infectious disease, with no specific treatment available. To identify novel human host cell targets important for dengue virus infection and replication, an image-based high-throughput siRNA assay screening of a human kinome siRNA library was conducted using human hep...
  8. Human regulatory B cells combine phenotypic and genetic hallmarks with a distinct differentiation fate.

    Journal of Immunology 193(5):2258 (2014) PMID 25080484

    Regulatory B cells (B-reg) produce IL-10 and suppress inflammation in both mice and humans, but limited data on the phenotype and function of these cells have precluded detailed assessment of their contribution to host immunity. In this article, we report that human B-reg cannot be defined based...
  9. Type I interferon signals in macrophages and dendritic cells control dengue virus infection: implications for a new mouse model to test dengue vaccines.

    Journal of Virology 88(13):7276 (2014) PMID 24741106 PMCID PMC4054447

    Dengue virus (DENV) infects an estimated 400 million people every year, causing prolonged morbidity and sometimes mortality. Development of an effective vaccine has been hampered by the lack of appropriate small animal models; mice are naturally not susceptible to DENV and only become infected i...
  10. Type I interferon signals in macrophages and dendritic cells control dengue virus infection: implications for a new mouse model to test dengue vaccines.

    Journal of Virology 88(13):7276 (2014) PMID 24741106 PMCID PMC4054447

    Dengue virus (DENV) infects an estimated 400 million people every year, causing prolonged morbidity and sometimes mortality. Development of an effective vaccine has been hampered by the lack of appropriate small animal models; mice are naturally not susceptible to DENV and only become infected i...
  11. Type I interferon signals in macrophages and dendritic cells control dengue virus infection: implications for a new mouse model to test dengue vaccines.

    Journal of Virology 88(13):7276 (2014) PMID 24741106 PMCID PMC4054447

    Dengue virus (DENV) infects an estimated 400 million people every year, causing prolonged morbidity and sometimes mortality. Development of an effective vaccine has been hampered by the lack of appropriate small animal models; mice are naturally not susceptible to DENV and only become infected i...
  12. Type I interferon signals in macrophages and dendritic cells control dengue virus infection: implications for a new mouse model to test dengue vaccines.

    Journal of Virology 88(13):7276 (2014) PMID 24741106 PMCID PMC4054447

    Dengue virus (DENV) infects an estimated 400 million people every year, causing prolonged morbidity and sometimes mortality. Development of an effective vaccine has been hampered by the lack of appropriate small animal models; mice are naturally not susceptible to DENV and only become infected i...
  13. A three-dimensional atlas of human dermal leukocytes, lymphatics, and blood vessels.

    Journal of Investigative Dermatology 134(4):965 (2014) PMID 24352044 PMCID PMC3961477

    Dendritic cells (DCs), macrophages (Mφ), and T cells are major components of the skin immune system, but their interstitial spatial organization is poorly characterized. Using four-channel whole-mount immunofluorescence staining of the human dermis, we demonstrated the three-dimensional distribu...
  14. Flavivirus RNA methylation.

    Journal of General Virology 95(Pt 4):763 (2014) PMID 24486628

    The 5' end of eukaryotic mRNA contains the type-1 (m7GpppNm) or type-2 (m7GpppNmNm) cap structure. Many viruses have evolved various mechanisms to develop their own capping enzymes (e.g. flavivirus and coronavirus) or to 'steal' caps from host mRNAs (e.g. influenza virus). Other viruses have dev...
  15. Activation of peripheral blood mononuclear cells by dengue virus infection depotentiates balapiravir.

    Journal of Virology 88(3):1740 (2014) PMID 24257621 PMCID PMC3911617

    In a recent clinical trial, balapiravir, a prodrug of a cytidine analog (R1479), failed to achieve efficacy (reducing viremia after treatment) in dengue patients, although the plasma trough concentration of R1479 remained above the 50% effective concentration (EC(50)). Here, we report experiment...
  16. Live attenuated vaccine: the first clinically approved dengue vaccine?

    Expert Review of Vaccines 13(2):185 (2014) PMID 24350687

    Dengue virus (DENV) is the most prevalent mosquito-borne viral pathogen in humans. There are 390 million human infections each year, with 96 million infections exhibiting disease symptoms. Currently, there is no clinically approved vaccine and antiviral for DENV. The four serotypes of DENV (DENV...
  17. Dengue serotype cross-reactive, anti-e protein antibodies confound specific immune memory for 1 year after infection.

    Frontiers in Immunology 5:388 (2014) PMID 25177321 PMCID PMC4132268

    Dengue virus has four serotypes and is endemic globally in tropical countries. Neither a specific treatment nor an approved vaccine is available, and correlates of protection are not established. The standard neutralization assay cannot differentiate between serotype-specific and serotype cross-...
  18. Rational design of a live attenuated dengue vaccine: 2'-o-methyltransferase mutants are highly attenuated and immunogenic in mice and macaques.

    PLoS Pathogens 9(8):e1003521 (2013) PMID 23935499 PMCID PMC3731252

    Dengue virus is transmitted by Aedes mosquitoes and infects at least 100 million people every year. Progressive urbanization in Asia and South-Central America and the geographic expansion of Aedes mosquito habitats have accelerated the global spread of dengue, resulting in a continuously increas...
  19. Plasmablasts generated during repeated dengue infection are virus glycoprotein-specific and bind to multiple virus serotypes.

    Journal of Immunology 189(12):5877 (2012) PMID 23152560

    Dengue virus immune protection is specific to the serotype encountered and is thought to persist throughout one's lifetime. Many serotype cross-reactive memory B cells isolated from humans with previous dengue infection are specific for the nonstructural and the prM structural viral proteins, an...
  20. Electrochemical impedance spectroscopy characterization of nanoporous alumina dengue virus biosensor.

    Bioelectrochemistry 88:15 (2012) PMID 22763420

    The Faradaic electrochemical impedance technique is employed to characterize the impedance change of a nanoporous alumina biosensor in response towards the specific binding of dengue serotype 2 (Denv2) viral particles to its serotype 2-specific immunoglobulin G antibody within the thin alumina l...