Post-polymerization crosstalk between the actin cytoskeleton and microtubule network.
BioArchitecture 6(3):53 (2016)
Cellular cytoskeletal systems play many pivotal roles in living organisms by controlling cell shape, division, and migration, which ultimately govern morphology, physiology, and functions of animals. Although the cytoskeletal systems are distinct and play different roles, there is growing eviden...
Mechanosensing via cell-matrix adhesions in 3D microenvironments.
Experimental Cell Research 343(1):60 (2016)
The extracellular matrix (ECM) microenvironment plays a central role in cell migration by providing physiochemical information that influences overall cell behavior. Much of this external information is accessed by direct interaction of the cell with ECM ligands and structures via integrin-based...
Fibroblasts Lead the Way: A Unified View of 3D Cell Motility.
Trends in Cell Biology 25(11):666 (2015)
Primary human fibroblasts are remarkably adaptable, able to migrate in differing types of physiological 3D tissue and on rigid 2D tissue culture surfaces. The crawling behavior of these and other vertebrate cells has been studied intensively, which has helped generate the concept of the cell mot...
Reproducibility and cell biology.
Journal of Cell Biology 209(2):191 (2015)
Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network.
Journal of Cell Biology 208(3):331 (2015)
Cell interactions with the extracellular matrix (ECM) can regulate multiple cellular activities and the matrix itself in dynamic, bidirectional processes. One such process is local proteolytic modification of the ECM. Invadopodia of tumor cells are actin-rich proteolytic protrusions that locally...
Pathogenic prion protein fragment (PrP106-126) promotes human immunodeficiency virus type-1 infection in peripheral blood monocyte-derived macrophages.
Virology 476:372 (2015)
Transfusion of blood and blood products contaminated with the pathogenic form of prion protein Prp(sc), thought to be the causative agent of variant a Creutzfeldt-Jakob disease (vCJD), may result in serious consequences in recipients with a compromised immune system, for example, as seen in HIV-...
Local 3D matrix microenvironment regulates cell migration through spatiotemporal dynamics of contractility-dependent adhesions.
Nature Communications 6:8720 (2015)
The physical properties of two-dimensional (2D) extracellular matrices (ECMs) modulate cell adhesion dynamics and motility, but little is known about the roles of local microenvironmental differences in three-dimensional (3D) ECMs. Here we generate 3D collagen gels of varying matrix microarchite...
Rho GEFs and GAPs: emerging integrators of extracellular matrix signaling.
Small GTPases 6(1):16 (2015)
Investigating cell migration in 3D settings has revealed that specific extracellular matrix environments require differential activities of the Rho GTPases for efficient migration. However, it is largely unknown how the activities of specific Rho GTPases are modulated to direct cell migration in...
Non-coding RNAs and heme oxygenase-1 in vaccinia virus infection.
Biochemical and Biophysical Research Communicat... 454(1):84 (2014)
Small nuclear RNAs (snRNAs) are <200 nucleotide non-coding uridylate-rich RNAs. Although the functions of many snRNAs remain undetermined, a population of snRNAs is produced during the early phase of infection of cells by vaccinia virus. In the present study, we demonstrate a direct correlation ...
Local and global dynamics of the basement membrane during branching morphogenesis require protease activity and actomyosin contractility.
Developmental Biology 394(2):197 (2014)
Many epithelial tissues expand rapidly during embryonic development while remaining surrounded by a basement membrane. Remodeling of the basement membrane is assumed to occur during branching morphogenesis to accommodate epithelial growth, but how such remodeling occurs is not yet clear. We repo...
An extracellular-matrix-specific GEF-GAP interaction regulates Rho GTPase crosstalk for 3D collagen migration.
Nature Cell Biology 16(9):909 (2014)
Rho-family GTPases govern distinct types of cell migration on different extracellular matrix proteins in tissue culture or three-dimensional (3D) matrices. We searched for mechanisms selectively regulating 3D cell migration in different matrix environments and discovered a form of Cdc42-RhoA cro...
Generation of compartmentalized pressure by a nuclear piston governs cell motility in a 3D matrix.
Science 345(6200):1062 (2014)
Cells use actomyosin contractility to move through three-dimensional (3D) extracellular matrices. Contractility affects the type of protrusions cells use to migrate in 3D, but the mechanisms are unclear. In this work, we found that contractility generated high-pressure lobopodial protrusions in ...
MYPT1 regulates contractility and microtubule acetylation to modulate integrin adhesions and matrix assembly.
Nature Communications 5:3510 (2014)
Although much is known about how individual cytoskeletal systems contribute to physiological processes such as cell migration and branching morphogenesis, little is known about how these different systems actively coordinate their functions after polymerization. Here we show that both fibroblast...
Regulation of cell adhesion and migration by cell-derived matrices.
Experimental Cell Research 319(16):2434 (2013)
Three-dimensional in vitro extracellular matrix models provide a physiological alternative to regular two-dimensional cell culture, though they lack the full diversity of molecular composition and physical properties of whole-animal systems. Cell-derived matrices are extracellular matrices that ...
Dimensions in cell migration.
Current Opinion in Cell Biology 25(5):642 (2013)
The importance of cell migration for both normal physiological functions and disease processes has been clear for the past 50 years. Although investigations of two-dimensional (2D) migration in regular tissue culture have elucidated many important molecular mechanisms, recent evidence suggests t...
Region-specific epithelial cell dynamics during branching morphogenesis.
Developmental Dynamics 242(9):1066 (2013)
Epithelial cells of developing embryonic organs, such as salivary glands, can display substantial motility during branching morphogenesis. Their dynamic movements and molecules involved in their migration are not fully characterized.
We generated transgenic mice expressing photo-convertible KikG...
ECM-modulated cellular dynamics as a driving force for tissue morphogenesis.
Current Opinion in Genetics & Development 23(4):408 (2013)
The extracellular matrix (ECM) plays diverse regulatory roles throughout development. Coordinate interactions between cells within a tissue and the ECM result in the dynamic remodeling of ECM structure. Both chemical signals and physical forces that result from such microenvironmental remodeling...
Heme oxygenase-1 induction alters chemokine regulation and ameliorates human immunodeficiency virus-type-1 infection in lipopolysaccharide-stimulated macrophages.
Biochemical and Biophysical Research Communicat... 435(3):373 (2013)
We have elucidated a putative mechanism for the host resistance against HIV-1 infection of primary human monocyte-derived macrophages (MDM) stimulated with lipopolysaccharide (LPS). We show that LPS-activated MDM both inhibited HIV-1 entry into the cells and were refractory to post-entry product...
At the leading edge of three-dimensional cell migration.
Journal of Cell Science 125(Pt 24):5917 (2012)
Cells migrating on flat two-dimensional (2D) surfaces use actin polymerization to extend the leading edge of the plasma membrane during lamellipodia-based migration. This mode of migration is not universal; it represents only one of several mechanisms of cell motility in three-dimensional (3D) e...
New dimensions in cell migration.
Nature Reviews: Molecular Cell Biology 13(11):743 (2012)
Studies of cell migration in three-dimensional (3D) cell culture systems and in vivo have revealed several differences when compared with cell migration in two dimensions, including their morphology and mechanical and signalling control. Here, researchers assess the contribution of 3D models to ...