1. Further increased production of free fatty acids by overexpressing a predicted transketolase gene of the pentose phosphate pathway in Aspergillus oryzae faaA disruptant.

    Bioscience, Biotechnology, and Biochemistry 80(9):1829 (2016) PMID 26998626

    Free fatty acids are useful as source materials for the production of biodiesel fuel and various chemicals such as pharmaceuticals and dietary supplements. Previously, we attained a 9.2-fold increase in free fatty acid productivity by disrupting a predicted acyl-CoA synthetase gene (faaA, AO0900...
  2. Increased production of free fatty acids in Aspergillus oryzae by disruption of a predicted acyl-CoA synthetase gene.

    Applied Microbiology and Biotechnology 99(7):3103 (2015) PMID 25564035

    Fatty acids are attractive molecules as source materials for the production of biodiesel fuel. Previously, we attained a 2.4-fold increase in fatty acid production by increasing the expression of fatty acid synthesis-related genes in Aspergillus oryzae. In this study, we achieved an additional i...
  3. Characterization of the biosynthetic gene cluster for the ribosomally synthesized cyclic peptide ustiloxin B in Aspergillus flavus.

    Fungal Genetics and Biology 68:23 (2014) PMID 24841822

    Ustiloxin B is a secondary metabolite known to be produced by Ustilaginoidea virens. In our previous paper, we observed the production of this compound by Aspergillus flavus, and identified two A. flavus genes responsible for ustiloxin B biosynthesis (Umemura et al., 2013). The compound is a cyc...
  4. Enhancing microbial metabolite and enzyme production: current strategies and challenges.

    Frontiers in Microbiology 5:718 (2014) PMID 25566228 PMCID PMC4270286

  5. Genome Sequence of the Mucoromycotina Fungus Umbelopsis isabellina, an Effective Producer of Lipids.

    Genome Announcements 2(1) (2014) PMID 24578268 PMCID PMC3937604

    Umbelopsis isabellina is a fungus in the subdivision Mucoromycotina, many members of which have been shown to be oleaginous and have become important organisms for producing oil because of their high level of intracellular lipid accumulation from various feedstocks. The genome sequence of U. isa...
  6. Increased production of fatty acids and triglycerides in Aspergillus oryzae by enhancing expressions of fatty acid synthesis-related genes.

    Applied Microbiology and Biotechnology 97(1):269 (2013) PMID 22733113

    Microbial production of fats and oils is being developed as a means of converting biomass to biofuels. Here we investigate enhancing expression of enzymes involved in the production of fatty acids and triglycerides as a means to increase production of these compounds in Aspergillus oryzae. Exami...
  7. Comparative genome analysis between Aspergillus oryzae strains reveals close relationship between sites of mutation localization and regions of highly divergent genes among Aspergillus species.

    DNA Research 19(5):375 (2012) PMID 22912434 PMCID PMC3473370

    Aspergillus oryzae has been utilized for over 1000 years in Japan for the production of various traditional foods, and a large number of A. oryzae strains have been isolated and/or selected for the effective fermentation of food ingredients. Characteristics of genetic alterations among the strai...
  8. Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae.

    Fungal Genetics and Biology 47(12):953 (2010) PMID 20849972

    Kojic acid is produced in large amounts by Aspergillus oryzae as a secondary metabolite and is widely used in the cosmetic industry. Glucose can be converted to kojic acid, perhaps by only a few steps, but no genes for the conversion have thus far been revealed. Using a DNA microarray, gene expr...
  9. Use of the Aspergillus oryzae actin gene promoter in a novel reporter system for exploring antifungal compounds and their target genes.

    Applied Microbiology and Biotechnology 87(5):1829 (2010) PMID 20464390

    Demand for novel antifungal drugs for medical and agricultural uses has been increasing because of the diversity of pathogenic fungi and the emergence of drug-resistant strains. Genomic resources for various living species, including pathogenic fungi, can be utilized to develop novel and effecti...
  10. Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound fromAspergillus oryzae

    Fungal Genetics and Biology 47(12):953 (2010) PMID 20849972

    Kojic acid is produced in large amounts by Aspergillus oryzae as a secondary metabolite and is widely used in the cosmetic industry. Glucose can be converted to kojic acid, perhaps by only a few steps, but no genes for the conversion have thus far been revealed. Using a DNA microarra...
  11. Genomics of industrial Aspergilli and comparison with toxigenic relatives.

    Food Additives & Contaminants: Part A - Chemist... 25(9):1147 (2008) PMID 18798040

    Aspergillus oryzae has been used in Japanese fermentation industries for more than a thousand years. The species produces large amounts of various hydrolytic enzymes and has been successfully applied to modern biotechnology. The size of the A. oryzae genome (37.5 Mb) is very close to that of A. ...
  12. Transcriptional regulation of genes on the non-syntenic blocks of Aspergillus oryzae and its functional relationship to solid-state cultivation.

    Fungal Genetics and Biology 45(2):139 (2008) PMID 17967552

    Transcriptome analysis revealed close relationship between solid-state cultivation and the transcriptional regulation of the genes on the non-syntenic blocks (NSBs), which were characterized by the comparison of Aspergillus oryzae genome with those of Aspergillus fumigatus and Aspergillus nidula...
  13. Transcriptional regulation of genes on the non-syntenic blocks ofAspergillus oryzaeand its functional relationship to solid-state cultivation

    Fungal Genetics and Biology 45(2):139 (2008)

    Transcriptome analysis revealed close relationship between solid-state cultivation and the transcriptional regulation of the genes on the non-syntenic blocks (NSBs), which were characterized by the comparison of Aspergillus oryzae genome with those of Aspergillus fumigatus and...
  14. The beta-1,3-exoglucanase gene exgA (exg1) of Aspergillus oryzae is required to catabolize extracellular glucan, and is induced in growth on a solid surface.

    Bioscience, Biotechnology, and Biochemistry 71(4):926 (2007) PMID 17420593

    The biological role of ExgA (Exg1), a secretory beta-1,3-exoglucanase of Aspergillus oryzae, and the expression pattern of the exgA (exg1) gene were analyzed. The exgA disruptant and the exgA-overexpressing mutant were constructed, and phenotypes of both mutants were compared. Higher mycelial gr...
  15. Improvement of the lectin-antibody enzyme immunoassay of the alphafetoprotein carbohydrate chain for automation with the enzyme immunoassay robot.

    Bioscience, Biotechnology, and Biochemistry 69(8):1616 (2005) PMID 16116296

    The lectin-antibody enzyme immunoassay of the alphafetoprotein-L3 carbohydrate chain, a tumor marker of liver cancer, has not been automated. We improved the technique of the assay for automation. Consequently, alphafetoprotein-L3 and total alphafetoprotein were detected with two lectins using a...
  16. Shigella Spa32 is an essential secretory protein for functional type III secretion machinery and uniformity of its needle length.

    Journal of Bacteriology 184(5):1244 (2002) PMID 11844752 PMCID PMC134865

    The Shigella type III secretion machinery is responsible for delivering to host cells the set of effectors required for invasion. The type III secretion complex comprises a needle composed of MxiH and MxiI and a basal body made up of MxiD, MxiG, and MxiJ. In S. flexneri, the needle length has a ...
  17. Purification and detection of Shigella type III secretion needle complex.

    Methods in Enzymology 358:385 (2002) PMID 12474402

  18. Purification and detection ofShigellatype III secretion needle complex

    Methods in Enzymology 358:385 (2002)

    This chapter describes the method for purifying the type III secretion complex, tentatively named the needle complex, from S. flexneri 2a strain VirG- mutan (M94). Because M94 contains the intact type III secretion system required for invasion but is defective in actin-based motility i...