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  1. Shigella Spa32 is an essential secretory protein for functional type III secretion machinery and uniformity of its needle length.

    Journal of Bacteriology 184(5):1244 (2002) PMID 11844752 PMCID PMC134865

    We show that Spa32, encoded by one of the spa genes, is an essential protein translocated via the type III secretion system and is involved in the control of needle length as well as type III secretion activity. When the spa32 gene was mutated, the type III secretion complexes possessed needles of v...
  2. Genomics of industrial Aspergilli and comparison with toxigenic relatives.

    Food Additives & Contaminants: Part A - Chemist... 25(9):1147 (2008) PMID 18798040

    Aspergillus oryzae has been used in Japanese fermentation industries for more than a thousand years. The species produces large amounts of various hydrolytic enzymes and has been successfully applied to modern biotechnology. The size of the A. oryzae genome (37.5 Mb) is very close to that of A. flav...
  3. Comparative genome analysis between Aspergillus oryzae strains reveals close relationship between sites of mutation localization and regions...

    DNA Research 19(5):375 (2012) PMID 22912434 PMCID PMC3473370

    We have sequenced the whole genome of an industrial fungal isolate, A. oryzae RIB326, by using a next-generation sequencing system and compared the data with those of A. oryzae RIB40, a wild-type strain sequenced in 2005. The aim of this study was to evaluate the mutation pressure on the non-synteni...
  4. Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus...

    Fungal Genetics and Biology 47(12):953 (2010) PMID 20849972

    Kojic acid is produced in large amounts by Aspergillus oryzae as a secondary metabolite and is widely used in the cosmetic industry. Glucose can be converted to kojic acid, perhaps by only a few steps, but no genes for the conversion have thus far been revealed. Using a DNA microarray, gene expressi...
  5. Enhancing microbial metabolite and enzyme production: current strategies and challenges.

    Frontiers in Microbiology 5:718 (2014) PMID 25566228 PMCID PMC4270286

  6. Purification and detection of Shigella type III secretion needle complex.

    Methods in Enzymology 358:385 (2002) PMID 12474402

  7. Improvement of the lectin-antibody enzyme immunoassay of the alphafetoprotein carbohydrate chain for automation with the enzyme immunoassay ...

    Bioscience, Biotechnology, and Biochemistry 69(8):1616 (2005) PMID 16116296

    We improved the technique of the assay for automation. Consequently, alphafetoprotein-L3 and total alphafetoprotein were detected with two lectins using an automatic paramagnetic bead handling robot. This indicates that the improved method is potentially applicable to the automated enzyme immunoassa...
  8. Genome Sequence of the Mucoromycotina Fungus Umbelopsis isabellina, an Effective Producer of Lipids.

    Genome Announcements 2(1) (2014) PMID 24578268 PMCID PMC3937604

    Umbelopsis isabellina is a fungus in the subdivision Mucoromycotina, many members of which have been shown to be oleaginous and have become important organisms for producing oil because of their high level of intracellular lipid accumulation from various feedstocks. The genome sequence of U. isabell...
  9. Increased production of fatty acids and triglycerides in Aspergillus oryzae by enhancing expressions of fatty acid synthesis-related genes.

    Applied Microbiology and Biotechnology 97(1):269 (2013) PMID 22733113

    We investigate enhancing expression of enzymes involved in the production of fatty acids and triglycerides as a means to increase production of these compounds in Aspergillus oryzae. Examination of the A. oryzae genome demonstrates that it contains two fatty acid synthases and several other genes th...
  10. Characterization of the biosynthetic gene cluster for the ribosomally synthesized cyclic peptide ustiloxin B in Aspergillus flavus.

    Fungal Genetics and Biology 68:23 (2014) PMID 24841822

    We observed the production of this compound by Aspergillus flavus, and identified two A. flavus genes responsible for ustiloxin B biosynthesis (Umemura et al., 2013). The compound is a cyclic tetrapeptide of Tyr-Ala-Ile-Gly, whose tyrosine is modified with a non-protein coding amino acid, norvaline....