1. Epoxyeicosatrienoic acids enhance embryonic haematopoiesis and adult marrow engraftment.

    Nature 523(7561):468 (2015) PMID 26201599

    Haematopoietic stem and progenitor cell (HSPC) transplant is a widely used treatment for life-threatening conditions such as leukaemia; however, the molecular mechanisms regulating HSPC engraftment of the recipient niche remain incompletely understood. Here we develop a competitive HSPC transpla...
  2. Screening for melanoma resistance genes in vivo.

    Pigment Cell & Melanoma Research 28(4):375 (2015) PMID 25818368

  3. TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis.

    PLoS Genetics 11(7):e1005346 (2015) PMID 26131719 PMCID PMC4488437

    In vertebrate definitive hematopoiesis, nascent hematopoietic stem/progenitor cells (HSPCs) migrate to and reside in proliferative hematopoietic microenvironment for transitory expansion. In this process, well-established DNA damage response pathways are vital to resolve the replication stress, ...
  4. Adenosine signaling promotes hematopoietic stem and progenitor cell emergence.

    Journal of Experimental Medicine 212(5):649 (2015) PMID 25870200 PMCID PMC4419349

    Hematopoietic stem cells (HSCs) emerge from aortic endothelium via the endothelial-to-hematopoietic transition (EHT). The molecular mechanisms that initiate and regulate EHT remain poorly understood. Here, we show that adenosine signaling regulates hematopoietic stem and progenitor cell (HSPC) d...
  5. Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence.

    Journal of Experimental Medicine 212(5):633 (2015) PMID 25870201 PMCID PMC4419355

    Fluid shear stress promotes the emergence of hematopoietic stem cells (HSCs) in the aorta-gonad-mesonephros (AGM) of the developing mouse embryo. We determined that the AGM is enriched for expression of targets of protein kinase A (PKA)-cAMP response element-binding protein (CREB), a pathway act...
  6. A CRISPR/Cas9 vector system for tissue-specific gene disruption in zebrafish.

    Developmental Cell 32(6):756 (2015) PMID 25752963 PMCID PMC4379706

    CRISPR/Cas9 technology of genome editing has greatly facilitated the targeted inactivation of genes in vitro and in vivo in a wide range of organisms. In zebrafish, it allows the rapid generation of knockout lines by simply injecting a guide RNA (gRNA) and Cas9 mRNA into one-cell stage embryos. ...
  7. A Zebrafish Model of Myelodysplastic Syndrome Produced through tet2 Genomic Editing.

    Molecular and Cellular Biology 35(5):789 (2015) PMID 25512612

    The ten-eleven translocation 2 gene (TET2) encodes a member of the TET family of DNA methylcytosine oxidases that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) to initiate the demethylation of DNA within genomic CpG islands. Somatic loss-of-function mutations of TET2 are freq...
  8. A Zebrafish Model of Myelodysplastic Syndrome Produced through tet2 Genomic Editing.

    Molecular and Cellular Biology 35(5):789 (2015) PMID 25512612 PMCID PMC4323485

    The ten-eleven translocation 2 gene (TET2) encodes a member of the TET family of DNA methylcytosine oxidases that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) to initiate the demethylation of DNA within genomic CpG islands. Somatic loss-of-function mutations of TET2 are freq...
  9. A Zebrafish Model of Myelodysplastic Syndrome Produced through tet2 Genomic Editing.

    Molecular and Cellular Biology 35(5):789 (2015) PMID 25512612

    The ten-eleven translocation 2 gene (TET2) encodes a member of the TET family of DNA methylcytosine oxidases that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) to initiate the demethylation of DNA within genomic CpG islands. Somatic loss-of-function mutations of TET2 are freq...
  10. A zebrafish model of myelodysplastic syndrome produced through tet2 genomic editing.

    Molecular and Cellular Biology 35(5):789 (2015) PMID 25512612 PMCID PMC4323485

    The ten-eleven translocation 2 gene (TET2) encodes a member of the TET family of DNA methylcytosine oxidases that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) to initiate the demethylation of DNA within genomic CpG islands. Somatic loss-of-function mutations of TET2 are freq...
  11. Notch1 acts via Foxc2 to promote definitive hematopoiesis via effects on hemogenic endothelium.

    Blood 125(9):1418 (2015) PMID 25587036 PMCID PMC4342355

    Hematopoietic and vascular development share many common features, including cell surface markers and sites of origin. Recent lineage-tracing studies have established that definitive hematopoietic stem and progenitor cells arise from vascular endothelial-cadherin(+) hemogenic endothelial cells o...
  12. Notch1 acts via Foxc2 to promote definitive hematopoiesis via effects on hemogenic endothelium.

    Blood 125(9):1418 (2015) PMID 25587036 PMCID PMC4342355

    Hematopoietic and vascular development share many common features, including cell surface markers and sites of origin. Recent lineage-tracing studies have established that definitive hematopoietic stem and progenitor cells arise from vascular endothelial-cadherin(+) hemogenic endothelial cells o...
  13. Hematopoietic stem cell arrival triggers dynamic remodeling of the perivascular niche.

    Cell 160(1-2):241 (2015) PMID 25594182 PMCID PMC4346256

    Hematopoietic stem and progenitor cells (HSPCs) can reconstitute and sustain the entire blood system. We generated a highly specific transgenic reporter of HSPCs in zebrafish. This allowed us to perform high-resolution live imaging on endogenous HSPCs not currently possible in mammalian bone mar...
  14. Hematopoietic stem cell arrival triggers dynamic remodeling of the perivascular niche.

    Cell 160(1-2):241 (2015) PMID 25594182

    Hematopoietic stem and progenitor cells (HSPCs) can reconstitute and sustain the entire blood system. We generated a highly specific transgenic reporter of HSPCs in zebrafish. This allowed us to perform high-resolution live imaging on endogenous HSPCs not currently possible in mammalian bone mar...
  15. Hematopoietic stem cell arrival triggers dynamic remodeling of the perivascular niche.

    Cell 160(1-2):241 (2015) PMID 25594182

    Hematopoietic stem and progenitor cells (HSPCs) can reconstitute and sustain the entire blood system. We generated a highly specific transgenic reporter of HSPCs in zebrafish. This allowed us to perform high-resolution live imaging on endogenous HSPCs not currently possible in mammalian bone mar...
  16. Hematopoietic stem cell arrival triggers dynamic remodeling of the perivascular niche.

    Cell 160(1-2):241 (2015) PMID 25594182

    Hematopoietic stem and progenitor cells (HSPCs) can reconstitute and sustain the entire blood system. We generated a highly specific transgenic reporter of HSPCs in zebrafish. This allowed us to perform high-resolution live imaging on endogenous HSPCs not currently possible in mammalian bone mar...
  17. A comparison of non-integrating reprogramming methods.

    Nature Biotechnology 33(1):58 (2015) PMID 25437882

    Human induced pluripotent stem cells (hiPSCs) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Her...
  18. A comparison of non-integrating reprogramming methods.

    Nature Biotechnology 33(1):58 (2015) PMID 25437882 PMCID PMC4329913

    Human induced pluripotent stem cells (hiPSCs) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Her...
  19. A comparison of non-integrating reprogramming methods.

    Nature Biotechnology 33(1):58 (2015) PMID 25437882

    Human induced pluripotent stem cells (hiPSCs) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Her...
  20. DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish.

    Journal of Hematology & Oncology 8(1):15 (2015) PMID 25886310 PMCID PMC4372312

    DNA methyltransferase 1 (Dnmt1) regulates expression of many critical genes through maintaining parental DNA methylation patterns on daughter DNA strands during mitosis. It is essential for embryonic development and diverse biological processes, including maintenance of hematopoietic stem and pr...