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  1. Glacial survival of boreal trees in northern Scandinavia.

    Science 335(6072):1083 (2012) PMID 22383845

    We show the presence of a rare mitochondrial DNA haplotype of spruce that appears unique to Scandinavia and with its highest frequency to the west-an area believed to sustain ice-free refugia during most of the last ice age. We further show the survival of DNA from this haplotype in lake sediments a...
  2. Ancient DNA analysis reveals woolly rhino evolutionary relationships.

    Molecular Phylogenetics and Evolution 28(3):485 (2003) PMID 12927133

    We have sequenced the entire 12S rRNA and partial cytochrome b (cyt b) genes of one 60-70,000-year-old sample, and partial 12S rRNA and cyt b sequences of two 40-45,000-year-old samples of the extinct woolly rhinoceros (Coelodonta antiquitatis). Based on these two mitochondrial markers, phylogenetic...
  3. The microcephalin ancestral allele in a Neanderthal individual.

    PLoS ONE 5(5):e10648 (2010) PMID 20498832 PMCID PMC2871044

    We report the first PCR amplification and high-throughput sequencing of nuclear DNA at the microcephalin (MCPH1) locus from Neanderthal individual from Mezzena Rockshelter (Monti Lessini, Italy). We show that a well-preserved Neanderthal fossil dated at approximately 50,000 years B.P., was homozygou...
  4. Islands in the ice: detecting past vegetation on Greenlandic nunataks using historical records and sedimentary ancient DNA meta-barcoding.

    Molecular Ecology 21(8):1980 (2012) PMID 21951625

    We have investigated the changes in plant composition at these nunataks using both the results of surveys of the flora over the last 130 years and through reconstruction of the vegetation from the end of the Holocene Thermal Maximum (5528 ± 75 cal year BP) using meta-barcoding of plant DNA...
  5. A 400,000-year-old mitochondrial genome questions phylogenetic relationships amongst archaic hominins: using the latest advances in ancient ...

    Bioessays 36(6):598 (2014) PMID 24706482

    By combining state-of-the-art approaches in ancient genomics, Meyer and co-workers have reconstructed the mitochondrial sequence of an archaic hominin that lived at Sierra de Atapuerca, Spain about 400,000 years ago. This achievement follows recent advances in molecular anthropology that delivered t...
  6. True single-molecule DNA sequencing of a pleistocene horse bone.

    Genome Research 21(10):1705 (2011) PMID 21803858 PMCID PMC3202287

    We report the results of the first "true single molecule sequencing" of ancient DNA. We generated 115.9 Mb and 76.9 Mb of DNA sequences from a permafrost-preserved Pleistocene horse bone using the Helicos HeliScope and Illumina GAIIx platforms, respectively. We find that the percentage of endogenous...
  7. Ligation bias in illumina next-generation DNA libraries: implications for sequencing ancient genomes.

    PLoS ONE 8(10):e78575 (2013) PMID 24205269 PMCID PMC3812280

    We first use fresh DNA extracts to demonstrate that library preparation based on adapter ligation at AT-overhangs are biased against DNA templates starting with thymine residues, contrarily to blunt-end adapter ligation. We observe the same bias on fresh DNA extracts sheared on Bioruptor, Covaris an...
  8. DNA from extinct giant lemurs links archaeolemurids to extant indriids.

    BMC Evolutionary Biology 8:121 (2008) PMID 18442367 PMCID PMC2386821

    We therefore used ancient DNA technology to address the phylogenetic status of the two archaeolemurid genera (Archaeolemur and Hadropithecus). Despite poor DNA preservation conditions in subtropical environments, we managed to recover 94- to 539-bp sequences for two mitochondrial genes among 5 subfo...
  9. A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional pla...

    Quaternary Science Reviews 75:161 (2013)

    We use 2nd generation sequencing technology on sedimentary ancient DNA (sedaDNA) from a lake in South Greenland to reconstruct the local floristic history around a low-arctic lake and compare the results with those previously obtained from pollen and macrofossils in the same lake. Thirty-eight of th...
  10. Ancient DNA analysis reveals divergence of the cave bear,Ursus spelaeus, and brown bear,Ursus arctos, lineages

    Current Biology 11(3):200 (2001)

    We previously amplified a short DNA fragment of the mitochondrial DNA control region (mt control region) of a 40,000-year-old Ursus spelaeus sample [4]. In this paper, we describe the DNA analysis of two mtDNA regions, the control region and the cytochrome b gene. Control region sequences were obtai...