Novel patient-derived xenograft and cell line models for therapeutic testing of pediatric liver cancer.
Journal of Hepatology 65(2):325 (2016)
Pediatric liver cancer is a rare but serious disease whose incidence is rising, and for which the therapeutic options are limited. Development of more targeted, less toxic therapies is hindered by the lack of an experimental animal model that captures the heterogeneity and metastatic capability ...
Asprosin, a Fasting-Induced Glucogenic Protein Hormone.
Cell 165(3):566 (2016)
Hepatic glucose release into the circulation is vital for brain function and survival during periods of fasting and is modulated by an array of hormones that precisely regulate plasma glucose levels. We have identified a fasting-induced protein hormone that modulates hepatic glucose release. It ...
Mapping Radiation Injury and Recovery in Bone Marrow Using 18F-FLT PET/CT and USPIO MRI in a Rat Model.
Journal of nuclear medicine : official publicat... 57(2):266 (2016)
We present and test the use of multimodality imaging as a topological tool to map the amount of the body exposed to ionizing radiation and the location of exposure, which are important indicators of survival and recovery. To achieve our goal, PET/CT imaging with 3'-deoxy-3'-(18)F-fluorothymidine...
Radiation-Induced Growth Retardation and Microstructural and Metabolite Abnormalities in the Hippocampus.
Neural plasticity 2016:3259621 (2016)
Cranial radiotherapy (CRT) increases survival in pediatric brain-tumor patients but can cause deleterious effects. This study evaluates the acute and long-term impact of CRT delivered during childhood/adolescence on the brain and body using a rodent model. Rats received CRT, either 4 Gy fraction...
Radiation combined injury models to study the effects of interventions and wound biomechanics.
Radiation Research 182(6):640 (2014)
In the event of a nuclear detonation, a considerable number of projected casualties will suffer from combined radiation exposure and burn and/or wound injury. Countermeasure assessment in the setting of radiation exposure combined with dermal injury is hampered by a lack of animal models in whic...
Neurogenesis, exercise, and cognitive late effects of pediatric radiotherapy.
Neural plasticity 2013:698528 (2013)
Brain cancer is a common type of childhood malignancy, and radiotherapy (RT) is a mainstay of treatment. RT is effective for tumor eradication, and survival rates are high. However, RT damages the brain and disrupts ongoing developmental processes, resulting in debilitating cognitive "late" effe...
Vascular-targeted photothermal therapy of an orthotopic murine glioma model.
Nanomedicine 7(8):1133 (2012)
To develop nanoshells for vascular-targeted photothermal therapy of glioma.
The ability of nanoshells conjugated to VEGF and/or poly(ethylene glycol) (PEG) to thermally ablate VEGF receptor-2-positive endothelial cells upon near-infrared laser irradiation was evaluated in vitro. Subsequent in vi...
Effects of irradiation on brain vasculature using an in situ tumor model.
International Journal of Radiation Oncology*Bio... 82(3):1075 (2012)
Damage to normal tissue is a limiting factor in clinical radiotherapy (RT). We tested the hypothesis that the presence of tumor alters the response of normal tissues to irradiation using a rat in situ brain tumor model.
Intravital microscopy was used with a rat cranial window to assess the in si...
Effect of radiation on the penetration of irinotecan in rat cerebrospinal fluid.
Cancer Chemotherapy and Pharmacology 68(3):721 (2011)
Anticancer agents are useful for treating brain tumors, but sub therapeutic concentrations due to decreased blood-brain barrier (BBB) penetration limit their effectiveness. This study evaluated the effect of cranial radiation on the pharmacokinetics of irinotecan in plasma and cerebrospinal flui...
Studies of biaxial mechanical properties and nonlinear finite element modeling of skin.
Molecular & Cellular Biomechanics 7(2):93 (2010)
The objective of this research is to conduct mechanical property studies of skin from two individual but potentially connected aspects. One is to determine the mechanical properties of the skin experimentally by biaxial tests, and the other is to use the finite element method to model the skin p...
A chitosan/beta-glycerophosphate thermo-sensitive gel for the delivery of ellagic acid for the treatment of brain cancer.
Biomaterials 31(14):4157 (2010)
We report here the development of a chitosan/beta-glycerophosphate(Ch/beta-GP) thermo-sensitive gel to deliver ellagic acid (EA) for cancer treatment. The properties of the Ch/beta-GP gels were characterized regarding chemical structure, surface morphology, and viscoelasticity. In vitro EA relea...
Improved intratumoral oxygenation through vascular normalization increases glioma sensitivity to ionizing radiation.
International Journal of Radiation Oncology*Bio... 76(5):1537 (2010)
Ionizing radiation, an important component of glioma therapy, is critically dependent on tumor oxygenation. However, gliomas are notable for areas of necrosis and hypoxia, which foster radioresistance. We hypothesized that pharmacologic manipulation of the typically dysfunctional tumor vasculatu...
IFN-beta restricts tumor growth and sensitizes alveolar rhabdomyosarcoma to ionizing radiation.
Molecular Cancer Therapeutics 9(3):761 (2010)
Ionizing radiation is an important component of multimodal therapy for alveolar rhabdomyosarcoma (ARMS). We sought to evaluate the ability of IFN-beta to enhance the activity of ionizing radiation. Rh-30 and Rh-41 ARMS cells were treated with IFN-beta and ionizing radiation to assess synergistic...
Modulation of collagen-induced arthritis by adenovirus-mediated intra-articular expression of modified collagen type II.
Arthritis research & therapy 12(4):R136 (2010)
Rheumatoid arthritis (RA) is a systemic disease manifested by chronic inflammation in multiple articular joints, including the knees and small joints of the hands and feet. We have developed a unique modification to a clinically accepted method for delivering therapies directly to the synovium. ...
Development of chitosan-ellagic acid films as a local drug delivery system to induce apoptotic death of human melanoma cells.
Journal of Biomedical Materials Research, Part ... 90(1):145 (2009)
This study was designed to develop a local chemotherapy device using chitosan as a local drug carrier and ellagic acid (EA) as an anticancer drug. We fabricated chitosan-ellagic acid (Ch-EA) films with concentrations of 0, 0.05, 0.1, 0.5, and 1% (w/v) of EA and examined the films using Fourier t...
Radiation-induced astrogliosis and blood-brain barrier damage can be abrogated using anti-TNF treatment.
International Journal of Radiation Oncology*Bio... 74(3):934 (2009)
In this article, we investigate the role of tumor necrosis factor-alpha (TNF) in the initiation of acute damage to the blood-brain barrier (BBB) and brain tissue following radiotherapy (RT) for CNS tumors.
Intravital microscopy and a closed cranial window technique were used to measure quantitat...
Cytokine and growth factor responses after radiotherapy for localized ependymoma.
International Journal of Radiation Oncology*Bio... 74(1):159 (2009)
To determine the time course and clinical significance of cytokines and peptide growth factors in pediatric patients with ependymoma treated with postoperative radiotherapy (RT).
We measured 15 cytokines and growth factors (fibroblast growth factor, epidermal growth factor, vascular endothelial ...
Continuous delivery of IFN-beta promotes sustained maturation of intratumoral vasculature.
Molecular Cancer Research 5(6):531 (2007)
IFNs have pleiotropic antitumor mechanisms of action. The purpose of this study was to further investigate the effects of IFN-beta on the vasculature of human xenografts in immunodeficient mice. We found that continuous, systemic IFN-beta delivery, established with liver-targeted adeno-associate...
Anti-TNFA (TNF-alpha) treatment abrogates radiation-induced changes in vacular density and tissue oxygenation.
Radiation Research 167(1):80 (2007)
Ionizing radiation significantly alters the structure and function of microvasculature, which regulates delivery of oxygen to brain tissue. Previous experimental and modeling studies have shown that tissue oxygenation patterns are significantly different in irradiated normal tissue compared to a...
A recombinant protein and a chemically synthesized peptide containing the active peptides of the platelet collagen receptors inhibit ferric chloride-induced thrombosis in a rat model.
Thrombosis Research 121(3):419 (2007)
We have previously reported that a recombinant protein (M(r) 47 kDa), which contains both active peptide of platelet receptors for types I and III collagen inhibits both types I and III collagen-induced platelet aggregation. In order to eliminate non-reactive portion of the protein, we have cons...