1. Novel patient-derived xenograft and cell line models for therapeutic testing of pediatric liver cancer.

    Journal of Hepatology 65(2):325 (2016) PMID 27117591

    Pediatric liver cancer is a rare but serious disease whose incidence is rising, and for which the therapeutic options are limited. Development of more targeted, less toxic therapies is hindered by the lack of an experimental animal model that captures the heterogeneity and metastatic capability ...
  2. Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases.

    Scientific reports 6:21264 (2016) PMID 26887909 PMCID PMC4757933

    Efficient gene editing is essential to fully utilize human pluripotent stem cells (hPSCs) in regenerative medicine. Custom endonuclease-based gene targeting involves two mechanisms of DNA repair: homology directed repair (HDR) and non-homologous end joining (NHEJ). HDR is the preferred mechanism...
  3. Photoswitchable Inhibitors of Microtubule Dynamics Optically Control Mitosis and Cell Death.

    Cell 162(2):403 (2015) PMID 26165941

    Small molecules that interfere with microtubule dynamics, such as Taxol and the Vinca alkaloids, are widely used in cell biology research and as clinical anticancer drugs. However, their activity cannot be restricted to specific target cells, which also causes severe side effects in chemotherapy...
  4. Human T-cell leukemia virus type 3 (HTLV-3) and HTLV-4 antisense-transcript-encoded proteins interact and transactivate Jun family-dependent transcription via their atypical bZIP motif.

    Journal of Virology 88(16):8956 (2014) PMID 24872589 PMCID PMC4136272

    Human T-cell leukemia virus types 3 and 4 (HTLV-3 and HTLV-4) are recently isolated retroviruses. We have previously characterized HTLV-3- and HTLV-4-encoded antisense genes, termed APH-3 and APH-4, respectively, which, in contrast to HBZ, the HTLV-1 homologue, do not contain a typical bZIP doma...
  5. In vitro differentiation and expansion of human pluripotent stem cell-derived pancreatic progenitors.

    The Review of Diabetic Studies 11(1):19 (2014) PMID 25148365 PMCID PMC4295798

    Recent progress in understanding stem cell biology has been remarkable, especially in deciphering signals that support differentiation towards tissue-specific lineages. This achievement positions us firmly at the beginning of an era of patient-specific regenerative medicine and human disease mod...
  6. HTLV-1 bZIP factor impedes the menin tumor suppressor and upregulates JunD-mediated transcription of the hTERT gene.

    Carcinogenesis 34(11):2664 (2013) PMID 23784080

    Telomerase activity in cancer cells is dependent on the transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene, encoding the catalytic subunit of human telomerase. We have shown previously that HTLV-1 basic leucine zipper (HBZ), a viral regulatory protein encoded b...
  7. Time-resolved emission spectra of 4-dimethylamino-4'-cyano-stilbene and resveratrol in high viscosity solvents and silica matrices.

    Spectrochimica Acta Part A: Molecular and Biomo... 115:111 (2013) PMID 23832219

    Time-resolved emission spectra of 4-dimethylamino-4'-cyano-stilbene (DMACS) and 3,5,4'-trihydroxy-stilbene (resveratrol, RSV) in propylene glycol and in rigid silica xerogel matrix at 23°C were studied. For the polar molecule DMACS in propylene glycol, a 66nm shift of maximum wavelength of emiss...
  8. Functional evaluation of ES cell-derived endodermal populations reveals differences between Nodal and Activin A-guided differentiation.

    Development 140(3):675 (2013) PMID 23293299 PMCID PMC3561793

    Embryonic stem (ES) cells hold great promise with respect to their potential to be differentiated into desired cell types. Of interest are organs derived from the definitive endoderm, such as the pancreas and liver, and animal studies have revealed an essential role for Nodal in development of t...
  9. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.

    Nature 491(7426):765 (2012) PMID 23041930

    One goal of regenerative medicine, to use stem cells to replace cells lost by injury or disease, depends on producing an excess of the relevant cell for study or transplantation. To this end, the stepwise differentiation of stem cells into specialized derivatives has been successful for some cel...
  10. The new generation of beta-cells: replication, stem cell differentiation, and the role of small molecules.

    The Review of Diabetic Studies 7(2):93 (2010) PMID 21060968 PMCID PMC2989782

    Diabetic patients suffer from the loss of insulin-secreting β-cells, or from an improper working β-cell mass. Due to the increasing prevalence of diabetes across the world, there is a compelling need for a renewable source of cells that could replace pancreatic β-cells. In recent years, several ...
  11. How to make beta cells?

    Current Opinion in Cell Biology 21(6):727 (2009) PMID 19781928 PMCID PMC4617625

    Insulin-producing beta cells are lost or insufficient in diabetic patients, presenting the medical challenge for new beta cells. Currently, there are three strategies that offer promise. One involves the generation of beta cells de novo by directing the differentiation of either embryonic stem c...
  12. Ebolavirus glycoprotein GP masks both its own epitopes and the presence of cellular surface proteins.

    Journal of Virology 83(18):9596 (2009) PMID 19587051 PMCID PMC2738224

    Ebolavirus (EBOV) is the etiological agent of a severe hemorrhagic fever with a high mortality rate. The spike glycoprotein (GP) is believed to be one of the major determinants of virus pathogenicity. In this study, we demonstrated the molecular mechanism responsible for the downregulation of su...
  13. c-Met confers protection against chronic liver tissue damage and fibrosis progression after bile duct ligation in mice.

    Gastroenterology 137(1):297 (2009) PMID 19208365

    The hepatocyte growth factor (HGF)/mesenchymal-epithelial transition factor (c-Met) system is an essential inducer of hepatocyte growth and proliferation. Although a fundamental role for the HGF receptor c-Met has been shown in acute liver regeneration, its cell-specific role in hepatocytes duri...
  14. Hepatocarcinogenesis in mice with a conditional knockout of the hepatocyte growth factor receptor c-Met.

    International Journal of Cancer 124(8):1767 (2009) PMID 19123478

    The receptor for the hepatocyte growth factor/scatter factor (HGF/SF), c-Met, plays a role in tumour promotion, progression and metastasis. In this study, we analysed chemically induced hepatocarcinogenesis in mice lacking a functional HGF receptor in their liver. Control and c-Met deficient mic...
  15. Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells.

    Cell Stem Cell 4(4):348 (2009) PMID 19341624 PMCID PMC4564293

    An essential step for therapeutic and research applications of stem cells is the ability to differentiate them into specific cell types. Endodermal cell derivatives, including lung, liver, and pancreas, are of interest for regenerative medicine, but efforts to produce these cells have been met w...
  16. A small molecule that directs differentiation of human ESCs into the pancreatic lineage.

    Nature Chemical Biology 5(4):258 (2009) PMID 19287398

    Stepwise differentiation from embryonic stem cells (ESCs) to functional insulin-secreting beta cells will identify key steps in beta-cell development and may yet prove useful for transplantation therapy for diabetics. An essential step in this schema is the generation of pancreatic progenitors--...
  17. Marked differences in differentiation propensity among human embryonic stem cell lines.

    Nature Biotechnology 26(3):313 (2008) PMID 18278034

    The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation...
  18. c-Met is essential for wound healing in the skin.

    Journal of Cell Biology 177(1):151 (2007) PMID 17403932 PMCID PMC2064119

    Wound healing of the skin is a crucial regenerative process in adult mammals. We examined wound healing in conditional mutant mice, in which the c-Met gene that encodes the receptor of hepatocyte growth factor/scatter factor was mutated in the epidermis by cre recombinase. c-Met-deficient kerati...
  19. Met provides essential signals for liver regeneration.

    PNAS 101(29):10608 (2004) PMID 15249655 PMCID PMC490025

    Genetic analysis in mice has demonstrated a crucial role of the Met tyrosine kinase receptor and its ligand, hepatocyte growth factor/scatter factor (HGF/SF), in development of the liver, muscle, and placenta. Here, we use conditional mutagenesis in mice to analyze the function of Met during liv...