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  1. Successful anti-scavenger receptor class B type I (SR-BI) monoclonal antibody therapy in humanized mice after challenge with HCV variants wi...

    Hepatology 60(5):1508 (2014) PMID 24797654

    We show, both in a preventative and postexposure setting, that humanized mice infected with HCV variants exhibiting increased in vitro resistance to SR-BI-targeting molecules remain responsive to anti-SR-BI mAb therapy in vivo. A 2-week antibody therapy readily cleared HCV RNA from the circulation o...
  2. AROS-29 is involved in adaptive response to oxidative stress.

    Free Radical Research 40(5):467 (2006) PMID 16551573

    We analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and mem...
  3. A genetically humanized mouse model for hepatitis C virus infection.

    Nature 474(7350):208 (2011) PMID 21654804 PMCID PMC3159410

    We attempted murine humanization via a genetic approach. Here we show that expression of two human genes is sufficient to allow HCV infection of fully immunocompetent inbred mice. We establish a precedent for applying mouse genetics to dissect viral entry and validate the role of scavenger receptor...
  4. High-avidity monoclonal antibodies against the human scavenger class B type I receptor efficiently block hepatitis C virus infection in the ...

    Journal of Virology 81(15):8063 (2007) PMID 17507483 PMCID PMC1951280

    We generated a panel of monoclonal antibodies (MAbs) against native human SR-B1. Two of them, 3D5 and C167, bound to conformation-dependent SR-B1 determinants and inhibited the interaction of sE2 with SR-B1. These antibodies efficiently blocked HCVcc infection of Huh-7.5 hepatoma cells in a dose-dep...
  5. Role of scavenger receptor class B type I in hepatitis C virus entry: kinetics and molecular determinants.

    Journal of Virology 84(1):34 (2010) PMID 19828610 PMCID PMC2798406

    I (SR-BI) is an essential receptor for hepatitis C virus (HCV) and a cell surface high-density-lipoprotein (HDL) receptor. The mechanism of SR-BI-mediated HCV entry, however, is not clearly understood, and the specific protein determinants required for the recognition of the virus envelope are not k...
  6. Ultrastructural analysis of hepatitis C virus particles.

    PNAS 110(23):9505 (2013) PMID 23690609 PMCID PMC3677472

    We generated an infectious HCV genome with an affinity tag fused to the E2 envelope glycoprotein. Using affinity grids, previously described to isolate proteins and macromolecular complexes for single-particle EM, we were able to purify enveloped particles directly from cell culture media. This appr...
  7. Expression of paramyxovirus V proteins promotes replication and spread of hepatitis C virus in cultures of primary human fetal liver cells.

    Hepatology 54(6):1901 (2011) PMID 22144107 PMCID PMC3233237

    We demonstrate that primary cultures of human fetal liver cells (HFLC) reliably support infection with laboratory strains of hepatitis C virus (HCV), although levels of virus replication vary significantly between different donor cell preparations and frequently decline in a manner suggestive of act...
  8. Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system.

    Nature Biotechnology 28(2):167 (2010) PMID 20118917 PMCID PMC2828266

    We develop a cell-based fluorescent reporter system that allows sensitive distinction of individual HCV-infected cells in live or fixed samples. We demonstrate use of this technology for several previously intractable applications, including live-cell imaging of viral propagation and host response,...
  9. Different requirements for scavenger receptor class B type I in hepatitis C virus cell-free versus cell-to-cell transmission.

    Journal of Virology 87(15):8282 (2013) PMID 23698298 PMCID PMC3719822

    We compared an adapted HCV genome, clone 2, characterized by superior cell-to cell spread, to its parental genome, J6/JFH-1, with the goal of elucidating the molecular mechanisms of HCV cell-to-cell transmission. We show that CD81 levels on the donor cells influence the efficiency of cell-to-cell sp...
  10. Completion of the entire hepatitis C virus life cycle in genetically humanized mice.

    Nature 501(7466):237 (2013) PMID 23903655 PMCID PMC3858853

    We previously showed that transient expression of these two human genes is sufficient to allow viral uptake into fully immunocompetent inbred mice. Here we demonstrate that transgenic mice stably expressing human CD81 and OCLN also support HCV entry, but innate and adaptive immune responses restrict...