1. Probing DNA helicase kinetics with temperature-controlled magnetic tweezers.

    Small 11(11):1273 (2015) PMID 25400244 PMCID PMC4473356

    Motor protein functions like adenosine triphosphate (ATP) hydrolysis or translocation along molecular substrates take place at nanometric scales and consequently depend on the amount of available thermal energy. The associated rates can hence be investigated by actively varying the temperature c...
  2. Magnetic tweezers: probing DNA helicase kinetics with temperature-controlled magnetic tweezers (small 11/2015).

    Small 11(11):1225 (2015) PMID 25767022

    The kinetics of double-stranded DNA processing are captured at single-molecule resolution by F. Moreno-Herrero and co-workers with a thermally stabilized magnetic tweezers microscope. An individual protein complex called "AddAB" links one end of a DNA molecule to a superparamagnetic bead that is...
  3. Specific and non-specific interactions of ParB with DNA: implications for chromosome segregation.

    Nucleic Acids Research 43(2):719 (2015) PMID 25572315 PMCID PMC4333373

    The segregation of many bacterial chromosomes is dependent on the interactions of ParB proteins with centromere-like DNA sequences called parS that are located close to the origin of replication. In this work, we have investigated the binding of Bacillus subtilis ParB to DNA in vitro using a var...
  4. Specific and non-specific interactions of ParB with DNA: implications for chromosome segregation.

    Nucleic Acids Research 43(2):719 (2015) PMID 25572315 PMCID PMC4333373

    The segregation of many bacterial chromosomes is dependent on the interactions of ParB proteins with centromere-like DNA sequences called parS that are located close to the origin of replication. In this work, we have investigated the binding of Bacillus subtilis ParB to DNA in vitro using a var...
  5. Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus.

    Biosensors and Bioelectronics 61:579 (2014) PMID 24953846 PMCID PMC4103019

    Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be used as a helicase probe in B. subtilis and clo...
  6. Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus.

    Biosensors and Bioelectronics 61:579 (2014) PMID 24953846 PMCID PMC4103019

    Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be used as a helicase probe in B. subtilis and clo...
  7. Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus

    Biosensors and Bioelectronics 61:579 (2014)

    Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be used as a helicase probe in B. subtil...
  8. Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus.

    Biosensors and Bioelectronics 61:579 (2014) PMID 24953846 PMCID PMC4103019

    Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be used as a helicase probe in B. subtilis and clo...
  9. Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus.

    Biosensors and Bioelectronics 61:579 (2014) PMID 24953846 PMCID PMC4103019

    Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be used as a helicase probe in B. subtilis and clo...
  10. Single molecule approaches to monitor the recognition and resection of double-stranded DNA breaks during homologous recombination.

    DNA Repair 20:119 (2014) PMID 24569169

    The fate of a cell depends on its ability to repair the many double-stranded DNA breaks (DSBs) that occur during normal metabolism. Improper DSB repair may result in genomic instability, cancer, or other genetic diseases. The repair of a DSB can be initiated by the recognition and resection of a...
  11. Recombination hotspots attenuate the coupled ATPase and translocase activities of an AddAB-type helicase-nuclease.

    Nucleic Acids Research 42(9):5633 (2014) PMID 24682829 PMCID PMC4027173

    In all domains of life, the resection of double-stranded DNA breaks to form long 3'-ssDNA overhangs in preparation for recombinational repair is catalyzed by the coordinated activities of DNA helicases and nucleases. In bacterial cells, this resection reaction is modulated by the recombination h...
  12. Recombination hotspots attenuate the coupled ATPase and translocase activities of an AddAB-type helicase-nuclease.

    Nucleic Acids Research 42(9):5633 (2014) PMID 24682829 PMCID PMC4027173

    In all domains of life, the resection of double-stranded DNA breaks to form long 3'-ssDNA overhangs in preparation for recombinational repair is catalyzed by the coordinated activities of DNA helicases and nucleases. In bacterial cells, this resection reaction is modulated by the recombination h...
  13. Structural basis for translocation by AddAB helicase-nuclease and its arrest at χ sites.

    Nature 508(7496):416 (2014) PMID 24670664 PMCID PMC3991583

    In bacterial cells, processing of double-stranded DNA breaks for repair by homologous recombination is dependent upon the recombination hotspot sequence χ (Chi) and is catalysed by either an AddAB- or RecBCD-type helicase-nuclease (reviewed in refs 3, 4). These enzyme complexes unwind and digest...
  14. Structural basis for translocation by AddAB helicase-nuclease and its arrest at χ sites.

    Nature 508(7496):416 (2014) PMID 24670664 PMCID PMC3991583

    In bacterial cells, processing of double-stranded DNA breaks for repair by homologous recombination is dependent upon the recombination hotspot sequence χ (Chi) and is catalysed by either an AddAB- or RecBCD-type helicase-nuclease (reviewed in refs 3, 4). These enzyme complexes unwind and digest...
  15. Probing the Kinetics of a Model Helicase-Nuclease with a Temperature-Controlled Magnetic Tweezers

    Biophysical Journal 106(2):393a (2014)

  16. Interactions Between the SMC-Complex, Spo0J and DNA

    Biophysical Journal 106(2):73a (2014)

  17. Condensation of DNA Mediated by the Bacterial Centromere Binding Protein Spo0J/ParB

    Biophysical Journal 106(2):429a (2014)

  18. Recombination hotspots attenuate the coupled ATPase and translocase activities of an AddAB-type helicase-nuclease.

    Nucleic Acids Research 42(9):5633 (2014) PMID 24682829 PMCID PMC4027173

    In all domains of life, the resection of double-stranded DNA breaks to form long 3'-ssDNA overhangs in preparation for recombinational repair is catalyzed by the coordinated activities of DNA helicases and nucleases. In bacterial cells, this resection reaction is modulated by the recombination h...
  19. On the mechanism of recombination hotspot scanning during double-stranded DNA break resection.

    PNAS 110(28):E2562 (2013) PMID 23798400 PMCID PMC3710824

    Double-stranded DNA break repair by homologous recombination is initiated by resection of free DNA ends to produce a 3'-ssDNA overhang. In bacteria, this reaction is catalyzed by helicase-nuclease complexes such as AddAB in a manner regulated by specific recombination hotspot sequences called Cr...
  20. On the mechanism of recombination hotspot scanning during double-stranded DNA break resection.

    PNAS 110(28):E2562 (2013) PMID 23798400 PMCID PMC3710824

    Double-stranded DNA break repair by homologous recombination is initiated by resection of free DNA ends to produce a 3'-ssDNA overhang. In bacteria, this reaction is catalyzed by helicase-nuclease complexes such as AddAB in a manner regulated by specific recombination hotspot sequences called Cr...