1. Heparin interaction with a receptor on hyperglycemic dividing cells prevents intracellular hyaluronan synthesis and autophagy responses in models of type 1 diabetes.

    Matrix Biology 48:36 (2015) PMID 25892563

    Previous studies and ongoing research indicate the importance of an interaction between a putative receptor on dividing cells in hyperglycemia and the non-reducing end motifs of heparin stored in mast cell secretory granules and how this interaction prevents activation of hyaluronan synthesis in...
  2. Preferential recognition of isocitrate dehydrogenase by a rabbit monoclonal antibody (ab124797) against the C-terminal peptide of RANKL.

    Journal of Immunological Methods 420:1 (2015) PMID 25771969

    A rabbit monoclonal antibody (Abcam ab124797), with high affinity for a synthetic peptide corresponding to the C-terminal region of the receptor activator of nuclear factor (NF)-κB ligand (RANKL), specifically recognizes a 37kDa protein by immunoblotting, in good agreement with the molecular mas...
  3. Lysosome-associated membrane proteins (LAMPs) regulate intracellular positioning of mitochondria in MC3T3-E1 cells.

    Experimental Cell Research 331(1):211 (2015) PMID 25246127

    The intracellular positioning of both lysosomes and mitochondria meets the requirements of degradation and energy supply, which are respectively the two major functions for cellular maintenance. The positioning of both lysosomes and mitochondria is apparently affected by the nutrient status of t...
  4. Expression of glucagon-like peptide-1 receptor and glucose‑dependent insulinotropic polypeptide receptor is regulated by the glucose concentration in mouse osteoblastic MC3T3-E1 cells.

    International Journal of Molecular Medicine 34(2):475 (2014) PMID 24866833

    Glucose-dependent insulinotropic polypeptide receptor (GIPR) and glucagon-like peptide-1 receptor (GLP‑1R) are incretin receptors that play important roles in regulating insulin secretion from pancreatic β cells. Incretin receptors are also thought to play a potential role in bone metabolism. Os...
  5. Sphingosine kinase 2 inhibitor SG-12 induces apoptosis via phosphorylation by sphingosine kinase 2.

    Bioorganic & Medicinal Chemistry Letters 23(7):2220 (2013) PMID 23434415

    Sphingosine kinase (SPHK), which catalyzes the phosphorylation of sphingosine to generate sphingosine 1-phosphate, has two mammalian isotypes, SPHK1 and SPHK2. Both isozymes are promising anti-cancer therapeutic targets. In this report, we found that SG-12, a synthetic analogue of sphingosine th...
  6. Degradation of MUC7 and MUC5B in human saliva.

    PLoS ONE 8(7):e69059 (2013) PMID 23874867 PMCID PMC3715446

    Two types of mucins, MUC7 and MUC5B constitute the major salivary glycoproteins, however their metabolic turnover has not been elucidated in detail to date. This study was conducted to examine turnover of MUC7 and MUC5B in saliva, by focusing on the relationship between their deglycosylation and...
  7. The proteoglycan repertoire of lymphoid cells.

    Glycoconjugate Journal 29(7):513 (2012) PMID 22777011 PMCID PMC3433671

    Proteoglycans have been studied to a limited extent in lymphoid cells. In this study we have investigated the expression of proteoglycans in B-cells, CD4+ T-cells, CD8+ T-cells, natural killer cells, as well as in nine different cell lines established from patients with lymphoid malignancies. Se...
  8. Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities.

    Structure 20(9):1585 (2012) PMID 22863568

    The leukocyte cell-surface antigen CD38 is the major nicotinamide adenide dinucleotide glycohydrolase in mammals, and its ectoenzyme activity is involved in calcium mobilization. CD38 is also a raft-dependent signaling molecule. CD38 forms a tetramer on the cell surface, but the structural basis...
  9. Syndecans reside in sphingomyelin-enriched low-density fractions of the plasma membrane isolated from a parathyroid cell line.

    PLoS ONE 7(3):e32351 (2012) PMID 22396758 PMCID PMC3291576

    Heparan sulfate proteoglycans (HSPGs) are one of the basic constituents of plasma membranes. Specific molecular interactions between HSPGs and a number of extracellular ligands have been reported. Mechanisms involved in controlling the localization and abundance of HSPG on specific domains on th...
  10. Relationship between oral malodor and glycosylated salivary proteins.

    Journal of medical and dental sciences 57(1):25 (2010) PMID 20437763

    Volatile sulfur compounds (VSCs), which are major sources of oral malodor, are produced by putrefactive activities of bacteria. Saliva provides easily degradable protein substrates, and most proteins are glycosylated. We hypothesized that oral malodor would be associated with enhanced proteolysi...
  11. Extraction and separation of proteoglycans.

    Glycoconjugate Journal 26(8):953 (2009) PMID 18493850

    Proteoglycans contain a unique carbohydrate component, glycosaminoglycan, which consists of repeating, typically sulfated disaccharides, and is capable of interacting with diverse molecules. Specific, clustered arrangements of sulfate on the glycosaminoglycan backbone form binding sites for many...
  12. Alteration of enzymatic properties of cell-surface antigen CD38 by agonistic anti-CD38 antibodies that prolong B cell survival and induce activation.

    International Immunopharmacology 8(1):59 (2008) PMID 18068101

    Leukocyte cell-surface antigen CD38 is a single-transmembrane protein. CD38 ligation by anti-CD38 antibodies triggers the growth or apoptosis of immune cells. Although the extracellular domain of CD38 has multifunctional catalytic activities including NAD(+) glycohydrolase and cyclase, the CD38-...
  13. Hypoxia/Notch signaling in primary culture of rat lymphatic endothelial cells.

    FEBS Letters 581(27):5220 (2007) PMID 17950285

    We have developed an improved intralumenal digestion method to get a long-term primary culture of rat lymphatic endothelial cells (rLECs) that maintained their original phenotypes. rLECs in vitro under hypoxia retained their original lymphatic properties observed in the thoracic duct. Blocking N...
  14. Nucleotide-sugar transporter SLC35D1 is critical to chondroitin sulfate synthesis in cartilage and skeletal development in mouse and human.

    Nature Medicine 13(11):1363 (2007) PMID 17952091

    Proteoglycans are a family of extracellular macromolecules comprised of glycosaminoglycan chains of a repeated disaccharide linked to a central core protein. Proteoglycans have critical roles in chondrogenesis and skeletal development. The glycosaminoglycan chains found in cartilage proteoglycan...
  15. Role of versican and hyaluronan in the differentiation of 3T3-L1 cells into preadipocytes and mature adipocytes.

    Matrix Biology 26(6):419 (2007) PMID 17513099

    We have previously shown that during the adipose conversion of these cells the culture medium changed its viscoelastic properties due to the presence of hyaluronan and a chondroitin sulfate proteoglycan [Calvo, J.C., Rodbard, D., Katki, A., Chernick, S., and Yanagishita, M., 1991. Differentiatio...
  16. Immunohistochemical localization of syndecan-1 in the dental follicle of postnatal mouse teeth.

    Journal of Periodontology 78(7):1322 (2007) PMID 17608588

    Syndecans are cell surface heparan sulfate proteoglycans that modulate the action of growth factors and extracellular matrix components. Syndecan-1 plays important roles during early tooth development, and it is expressed in the dental follicle of fetal tooth germ. However, no studies have follo...
  17. Syntaxin6 separates from GM1a-rich membrane microdomain during granule maturation.

    Biochemical and Biophysical Research Communicat... 357(4):1071 (2007) PMID 17459336

    Since it was reported that components of immature secretory granules (ISGs) are different from those of mature secretory granules (MSGs) in rat parotid acinar cells, we have been considering that components of secretory granules (SGs) change dynamically during granule maturation. As the first st...
  18. Relationship between total salivary protein content and volatile sulfur compounds levels in malodor patients.

    Oral Surgery, Oral Medicine, Oral Pathology, Or... 103(5):655 (2007) PMID 17150382

    The purpose of this study was to evaluate the relationship between total salivary protein and VSC levels. A patient group comprised 67 patients from the Fresh Breath Clinic of the Dental Hospital, Tokyo Medical and Dental University, with 18 healthy subjects as a control group. Gas chromatograph...
  19. Hypoxia/Notch signaling in primary culture of rat lymphatic endothelial cells

    FEBS Letters 581(27):5220 (2007)

    We have developed an improved intralumenal digestion method to get a long-term primary culture of rat lymphatic endothelial cells (rLECs) that maintained their original phenotypes. rLECs in vitro under hypoxia retained their original lymphatic properties observed in the thoracic duct. Block...
  20. Syntaxin6 separates from GM1a-rich membrane microdomain during granule maturation

    Biochemical and Biophysical Research Communicat... 357(4):1071 (2007)

    Since it was reported that components of immature secretory granules (ISGs) are different from those of mature secretory granules (MSGs) in rat parotid acinar cells, we have been considering that components of secretory granules (SGs) change dynamically during granule maturation. As the fir...