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  1. Successful sterile vitrification of human zygotes and blastocysts

    Fertility and Sterility 80:142 (2003)

  2. Highly efficient vitrification method for cryopreservation of human oocytes.

    Reproductive BioMedicine Online 11(3):300 (2005) PMID 16176668

    Two experiments were performed to develop a method to cryopreserve MII human oocytes. In the first experiment, three vitrification methods were compared using bovine MII oocytes with regard to their developmental competence after cryopreservation: (i) vitrification within 0.25-ml plastic straws foll...
  3. Clinical efficiency of vitrification on blastocysts transfer cycles

    Fertility and Sterility 80:63 (2003)

  4. Effects of cryodevice type and donors' sexual maturity on vitrification of minke whale (Balaenoptera bonaerensis) oocytes at germinal vesicl...

    Zygote 12(4):333 (2004) PMID 15751543

    These results indicate that the Cryotop is a better device than the OPS for vitrification of immature oocytes from adult minke whales....
  5. Characterization of the zona pellucida glycoproteins from bovine ovarian and fertilized eggs

    Biochimica et Biophysica Acta (BBA) - General S... 1201(1):7 (1994) PMID 7918585

    Bovine zone pellucida (ZP) glycorproteins from ovarian egg emerged as three bands with molecular mass of 78 kDa, 64 kDa and 21 kDa in SDS-PAGE under reducing conditions. Endo-β-galactosidase (EβG) digestion of the glycoproteins yielded five products with molecular mass of 76 kDa (EβG-76), 68 kD...
  6. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method.

    Theriogenology 67(1):73 (2007) PMID 17055564

    Vitrification is frequently referred to as a novel technology of cryopreservation in embryology, although some young embryologists were born after its first successful application. Unfortunately, in spite of the accumulated evidence regarding its enormous potential value, most domestic animal and hu...
  7. Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes.

    Reproductive BioMedicine Online 18(3):360 (2009) PMID 19298735

    High hydrostatic pressure has been reported to improve the fertilizing or developmental ability of mammalian spermatozoa, oocytes and embryos. This study investigated the effect of another stress, temporarily increased NaCl concentration, on cryotolerance and developmental competence of porcine oocy...
  8. Cryopreservation of porcine embryos derived from in vitro-matured oocytes.

    Biology of Reproduction 71(2):432 (2004) PMID 15044264

    These results clearly confirm that porcine embryos derived from IVM oocytes can be effectively cryopreserved with high embryo survival using the MVC method in conjunction with delipation....
  9. High survival rate of vitrified human oocytes results in clinical pregnancy

    Fertility and Sterility 80(1):223 (2003) PMID 12849831

  10. Osmotic stress induced by sodium chloride, sucrose or trehalose improves cryotolerance and developmental competence of porcine oocytes.

    Reproduction, Fertility and Development 21(2):338 (2009) PMID 19210925

    We compared the effects of NaCl with those of concentrated solutions of two non-permeable osmotic agents, namely sucrose and trehalose, on the cryotolerance and developmental competence of porcine oocytes. In Experiment 1, porcine in vitro-matured cumulus-oocyte complexes (COCs; n = 1200) were expos...