1. Oophorectomy for fertility preservation via reduced-port laparoscopic surgery.

    Surgical Innovation 20(3):219 (2013) PMID 22696023

    For fertility preservation of women patients scheduled to undergo chemotherapy or radiotherapy, unilateral oophorectomy was performed, and the ovary was cryopreserved. Two-port surgery was conducted in 3 patients, and single-port surgery using a single-incision laparoscopic surgery port in 3. An...
  2. Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center.

    Journal of Reproduction and Development 57(3):428 (2011) PMID 21325738

    This study was designed to investigate whether freeze-dried (FD) bull spermatozoa maintained the function of the microtubule-organizing center (MTOC) after rehydration and intracytoplasmic sperm injection (ICSI). In a preliminary attempt, the cleavage and blastocyst formation rates in FD-ICSI zy...
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  3. Production of fertile zebrafish (Danio rerio) possessing germ cells (gametes) originated from primordial germ cells recovered from vitrified embryos.

    Reproduction 139(4):733 (2010) PMID 20154175

    This study aimed to produce fertile zebrafish (Danio rerio) possessing germ cells (gametes) that originated from cryopreserved primordial germ cells (PGCs). First, to improve the vitrification procedure of PGCs in segmentation stage embryos, dechorionated yolk-intact and yolk-removed embryos, th...
  4. Duration of fertility after fresh and frozen ovary transplantation

    Fertility and Sterility 94(6):2191 (2010) PMID 20171622

    Objective To evaluate the function of human ovarian transplants.
  5. Duration of fertility after fresh and frozen ovary transplantation

    Fertility and Sterility 94(6):2191 (2010) PMID 20171622

    Objective To evaluate the function of human ovarian transplants.
  6. Duration of fertility after fresh and frozen ovary transplantation

    Fertility and Sterility 94(6):2191 (2010) PMID 20171622

    Objective To evaluate the function of human ovarian transplants.
  7. Successful vitrification of bovine and human ovarian tissue.

    Reproductive BioMedicine Online 18(4):568 (2009) PMID 19401001

    Ovariectomy and ovarian tissue cryopreservation has the potential to preserve the natural fertility of cancer patients prior to sterilizing chemo- and radiotherapies. Ovarian tissue cryopreservation with the conventional slow-freezing method has yielded limited success, partly because of oocyte ...
  8. Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes.

    Reproductive BioMedicine Online 18(3):360 (2009) PMID 19298735

    High hydrostatic pressure has been reported to improve the fertilizing or developmental ability of mammalian spermatozoa, oocytes and embryos. This study investigated the effect of another stress, temporarily increased NaCl concentration, on cryotolerance and developmental competence of porcine ...
  9. Successful vitrification of bovine and human ovarian tissue

    Reproductive BioMedicine Online 18(4):568 (2009)

    Ovariectomy and ovarian tissue cryopreservation has the potential to preserve the natural fertility of cancer patients prior to sterilizing chemo- and radiotherapies. Ovarian tissue cryopreservation with the conventional slow-freezing method has yielded limited success, partly because of oo...
  10. Osmotic stress induced by sodium chloride, sucrose or trehalose improves cryotolerance and developmental competence of porcine oocytes.

    Reproduction, Fertility and Development 21(2):338 (2009) PMID 19210925

    Exposure of porcine oocytes to increased concentrations of NaCl prior to manipulation has been reported not only to increase cryotolerance after vitrification, but also to improve developmental competence after somatic cell nuclear transfer (SCNT). In the present study we compared the effects of...
  11. Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes

    Reproductive BioMedicine Online 18(3):360 (2009)

    High hydrostatic pressure has been reported to improve the fertilizing or developmental ability of mammalian spermatozoa, oocytes and embryos. This study investigated the effect of another stress, temporarily increased NaCl concentration, on cryotolerance and developmental competence of por...
  12. The Well-of-the-Well system: an efficient approach to improve embryo development.

    Reproductive BioMedicine Online 17(1):73 (2008) PMID 18616894

    Transfer of human embryos at the blastocyst stage may offer considerable benefits including an increased implantation rate and a decreased risk of multiple pregnancies; however, blastocyst culture requires an efficient and reliable in-vitro embryo culture system. In this study, the effect of the...
  13. The Well-of-the-Well system: an efficient approach to improve embryo development

    Reproductive BioMedicine Online 17(1):73 (2008)

    Transfer of human embryos at the blastocyst stage may offer considerable benefits including an increased implantation rate and a decreased risk of multiple pregnancies; however, blastocyst culture requires an efficient and reliable in-vitro embryo culture system. In this study, the effect o...
  14. Comparison of efficiency of open pulled straw (OPS) and Cryotop vitrification for cryopreservation of in vitro matured pig oocytes.

    Cryo letters 29(4):315 (2008) PMID 19137194

    During the past few years vitrification has been acknowledged as a viable alternative to traditional slow-rate freezing in both animal and human embryology. However, few data are available regarding the comparative efficiency of published and commercially available vitrification methods. The pur...
  15. Production of the first offspring from oocytes derived from fresh and cryopreserved pre-antral follicles of adult mice.

    Reproductive BioMedicine Online 14(6):693 (2007) PMID 17579980

    Although mammalian ovaries contain hundreds of thousands of pre-antral follicles, fewer than 1% of these reach maturity and ovulation. Obtaining immature eggs from the pre-antral follicles of ovarian tissue could increase the possibility of preserving fertility in women undergoing anti-cancer tr...
  16. Production of the first offspring from oocytes derived from fresh and cryopreserved pre-antral follicles of adult mice

    Reproductive BioMedicine Online 14(6):693 (2007)

    Although mammalian ovaries contain hundreds of thousands of pre-antral follicles, fewer than 1% of these reach maturity and ovulation. Obtaining immature eggs from the pre-antral follicles of ovarian tissue could increase the possibility of preserving fertility in women undergoing anti-canc...
  17. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method.

    Theriogenology 67(1):73 (2007) PMID 17055564

    Vitrification is frequently referred to as a novel technology of cryopreservation in embryology, although some young embryologists were born after its first successful application. Unfortunately, in spite of the accumulated evidence regarding its enormous potential value, most domestic animal an...
  18. Improving cryopreservation systems.

    Theriogenology 65(1):236 (2006) PMID 16289262

    Cryopreservation of oocytes and embryos is a crucial step for widespread practical application of other techniques in domestic animal embryology. However, in contrast to the rapid development of procedures in the eighties and early nineties of the last century, new advancements with profound pra...
  19. Improving cryopreservation systems

    Theriogenology 65(1):236 (2006)

    Cryopreservation of oocytes and embryos is a crucial step for widespread practical application of other techniques in domestic animal embryology. However, in contrast to the rapid development of procedures in the eighties and early nineties of the last century, new advancements with profoun...
  20. Growth and maturation of follicles and oocytes following xenotransplantation of porcine ovarian tissues and in vitro maturation.

    Journal of Reproduction and Development 51(6):741 (2005) PMID 16227672

    This is the first report to show morphological evidence of in vitro maturation of oocytes recovered from xenotransplanted antral follicles. To develop a suitable tool for studing the growth and maturation of follicles and oocytes, we xenotransplanted small pieces of ovarian cortical tissue from ...
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