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  1. Oophorectomy for fertility preservation via reduced-port laparoscopic surgery.

    Surgical Innovation 20(3):219 (2013) PMID 22696023

    For fertility preservation of women patients scheduled to undergo chemotherapy or radiotherapy, unilateral oophorectomy was performed, and the ovary was cryopreserved. Two-port surgery was conducted in 3 patients, and single-port surgery using a single-incision laparoscopic surgery port in 3. An...
  2. Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center.

    Journal of Reproduction and Development 57(3):428 (2011) PMID 21325738

    This study was designed to investigate whether freeze-dried (FD) bull spermatozoa maintained the function of the microtubule-organizing center (MTOC) after rehydration and intracytoplasmic sperm injection (ICSI). In a preliminary attempt, the cleavage and blastocyst formation rates in FD-ICSI zy...
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  3. Production of fertile zebrafish (Danio rerio) possessing germ cells (gametes) originated from primordial germ cells recovered from vitrified embryos.

    Reproduction 139(4):733 (2010) PMID 20154175

    This study aimed to produce fertile zebrafish (Danio rerio) possessing germ cells (gametes) that originated from cryopreserved primordial germ cells (PGCs). First, to improve the vitrification procedure of PGCs in segmentation stage embryos, dechorionated yolk-intact and yolk-removed embryos, th...
  4. Duration of fertility after fresh and frozen ovary transplantation

    Fertility and Sterility 94(6):2191 (2010) PMID 20171622

    Objective To evaluate the function of human ovarian transplants.
  5. Duration of fertility after fresh and frozen ovary transplantation

    Fertility and Sterility 94(6):2191 (2010) PMID 20171622

    Objective To evaluate the function of human ovarian transplants.
  6. Duration of fertility after fresh and frozen ovary transplantation

    Fertility and Sterility 94(6):2191 (2010) PMID 20171622

    Objective To evaluate the function of human ovarian transplants.
  7. Successful vitrification of bovine and human ovarian tissue.

    Reproductive BioMedicine Online 18(4):568 (2009) PMID 19401001

    Ovariectomy and ovarian tissue cryopreservation has the potential to preserve the natural fertility of cancer patients prior to sterilizing chemo- and radiotherapies. Ovarian tissue cryopreservation with the conventional slow-freezing method has yielded limited success, partly because of oocyte ...
  8. Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes.

    Reproductive BioMedicine Online 18(3):360 (2009) PMID 19298735

    High hydrostatic pressure has been reported to improve the fertilizing or developmental ability of mammalian spermatozoa, oocytes and embryos. This study investigated the effect of another stress, temporarily increased NaCl concentration, on cryotolerance and developmental competence of porcine ...
  9. Successful vitrification of bovine and human ovarian tissue

    Reproductive BioMedicine Online 18(4):568 (2009)

    Ovariectomy and ovarian tissue cryopreservation has the potential to preserve the natural fertility of cancer patients prior to sterilizing chemo- and radiotherapies. Ovarian tissue cryopreservation with the conventional slow-freezing method has yielded limited success, partly because of oo...
  10. Osmotic stress induced by sodium chloride, sucrose or trehalose improves cryotolerance and developmental competence of porcine oocytes.

    Reproduction, Fertility and Development 21(2):338 (2009) PMID 19210925

    Exposure of porcine oocytes to increased concentrations of NaCl prior to manipulation has been reported not only to increase cryotolerance after vitrification, but also to improve developmental competence after somatic cell nuclear transfer (SCNT). In the present study we compared the effects of...