1. Prediction of Growth Factor-Dependent Cleft Formation During Branching Morphogenesis Using A Dynamic Graph-Based Growth Model.

    Computational Biology and Bioinformatics, IEEE/... 13(2):350 (2016) PMID 27070978 PMCID PMC4917296

    This study considers the problem of describing and predicting cleft formation during the early stages of branching morphogenesis in mouse submandibular salivary glands (SMG) under the influence of varied concentrations of epidermal growth factors (EGF). Given a time-lapse video of a growing SMG,...
  2. Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjögren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model.

    Anatomical Record. Part A: Advances in Integrat... 298(9):1622 (2015) PMID 26179322 PMCID PMC4888376

    Sjögren's syndrome (SS), an autoimmune exocrinopathy, is associated with dysfunction of the secretory salivary gland epithelium, leading to xerostomia. The etiology of SS disease progression is poorly understood as it is typically not diagnosed until late stage. Since mouse models allow the stud...
  3. The contribution of specific cell subpopulations to submandibular salivary gland branching morphogenesis.

    Current Opinion in Genetics & Development 32:47 (2015) PMID 25706196 PMCID PMC4470835

    Branching morphogenesis is the developmental program responsible for generating a large surface to volume ratio in many secretory and absorptive organs. To accomplish branching morphogenesis, spatiotemporal regulation of specific cell subpopulations is required. Here, we review recent studies th...
  4. Heterotypic control of basement membrane dynamics during branching morphogenesis.

    Developmental Biology 401(1):103 (2015) PMID 25527075 PMCID PMC4465071

    Many mammalian organs undergo branching morphogenesis to create highly arborized structures with maximized surface area for specialized organ function. Cooperative cell-cell and cell-matrix adhesions that sculpt the emerging tissue architecture are guided by dynamic basement membranes. Propertie...
  5. TGFβ signaling promotes matrix assembly during mechanosensitive embryonic salivary gland restoration.

    Matrix Biology 43:109 (2015) PMID 25652203 PMCID PMC4899049

    Mechanical properties of the microenvironment regulate cell morphology and differentiation within complex organs. However, methods to restore morphogenesis and differentiation in organs in which compliance is suboptimal are poorly understood. We used mechanosensitive mouse salivary gland organ e...
  6. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis.

    Molecular Biology of the Cell 25(16):2393 (2014) PMID 24966172 PMCID PMC4142612

    Coordinated actin microfilament and microtubule dynamics is required for salivary gland development, although the mechanisms by which they contribute to branching morphogenesis are not defined. Because LIM kinase (LIMK) regulates both actin and microtubule organization, we investigated the role ...
  7. Biocompatible tissue scaffold compliance promotes salivary gland morphogenesis and differentiation.

    Tissue Engineering, Part A: Tissue Engineering 20(11-12):1632 (2014) PMID 24410370 PMCID PMC4029047

    Substrate compliance is reported to alter cell phenotype, but little is known about the effects of compliance on cell development within the context of a complex tissue. In this study, we used 0.48 and 19.66 kPa polyacrylamide gels to test the effects of the substrate modulus on submandibular sa...
  8. Microfluidic platform for the elastic characterization of mouse submandibular glands by atomic force microscopy.

    Biosensors 4(1):18 (2014) PMID 25587408 PMCID PMC4264367

    The ability to characterize the microscale mechanical properties of biological materials has the potential for great utility in the field of tissue engineering. The development and morphogenesis of mammalian tissues are known to be guided in part by mechanical stimuli received from the local env...
  9. Integrins promote cytokinesis through the RSK signaling axis.

    Journal of Cell Science 127(Pt 3):534 (2014) PMID 24284076 PMCID PMC4007764

    Cytokinesis is the final stage in cell division. Although integrins can regulate cytokinesis, the mechanisms involved are not fully understood. In this study, we demonstrate that integrin-regulated ERK (extracellular signal-related kinase) and RSK (p90 ribosomal S6 kinase) signaling promotes suc...
  10. Salivary gland cell differentiation and organization on micropatterned PLGA nanofiber craters.

    Biomaterials 34(28):6773 (2013) PMID 23777914 PMCID PMC3755621

    There is a need for an artificial salivary gland as a long-term remedy for patients suffering from salivary hypofunction, a leading cause of chronic xerostomia (dry mouth). Current salivary gland tissue engineering approaches are limited in that they either lack sufficient physical cues and surf...
  11. Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue.

    PNAS 110(29):11982 (2013) PMID 23818604 PMCID PMC3718135

    Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances in understanding the biological basis of health and disease. Here we present a multiplexed fluorescence microscopy method (MxIF) for quantitative, s...
  12. Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation.

    Biology Open 2(5):439 (2013) PMID 23789091 PMCID PMC3654261

    Epithelial organ morphogenesis involves reciprocal interactions between epithelial and mesenchymal cell types to balance progenitor cell retention and expansion with cell differentiation for evolution of tissue architecture. Underlying submandibular salivary gland branching morphogenesis is the ...
  13. Genetic modification and recombination of salivary gland organ cultures.

    Journal of Visualized Experiments (71):e50060 (2013) PMID 23407326 PMCID PMC3582689

    Branching morphogenesis occurs during the development of many organs, and the embryonic mouse submandibular gland (SMG) is a classical model for the study of branching morphogenesis. In the developing SMG, this process involves iterative steps of epithelial bud and duct formation, to ultimately ...
  14. Cell-based multi-parametric model of cleft progression during submandibular salivary gland branching morphogenesis.

    PLoS computational biology 9(11):e1003319 (2013) PMID 24277996 PMCID PMC3836695

    Cleft formation during submandibular salivary gland branching morphogenesis is the critical step initiating the growth and development of the complex adult organ. Previous experimental studies indicated requirements for several epithelial cellular processes, such as proliferation, migration, cel...
  15. Selective functionalization of nanofiber scaffolds to regulate salivary gland epithelial cell proliferation and polarity.

    Biomaterials 33(33):8372 (2012) PMID 22938763 PMCID PMC3491572

    Epithelial cell types typically lose apicobasal polarity when cultured on 2D substrates, but apicobasal polarity is required for directional secretion by secretory cells, such as salivary gland acinar cells. We cultured salivary gland epithelial cells on poly(lactic-co-glycolic acid) (PLGA) nano...
  16. The regulation of focal adhesion complex formation and salivary gland epithelial cell organization by nanofibrous PLGA scaffolds.

    Biomaterials 33(11):3175 (2012) PMID 22285464 PMCID PMC3324334

    Nanofiber scaffolds have been useful for engineering tissues derived from mesenchymal cells, but few studies have investigated their applicability for epithelial cell-derived tissues. In this study, we generated nanofiber (250 nm) or microfiber (1200 nm) scaffolds via electrospinning from the po...
  17. ROCK1-directed basement membrane positioning coordinates epithelial tissue polarity.

    Development 139(2):411 (2012) PMID 22186730 PMCID PMC3243099

    The basement membrane is crucial for epithelial tissue organization and function. However, the mechanisms by which basement membrane is restricted to the basal periphery of epithelial tissues and the basement membrane-mediated signals that regulate coordinated tissue organization are not well de...
  18. Multiscale feature analysis of salivary gland branching morphogenesis.

    PLoS ONE 7(3):e32906 (2012) PMID 22403724 PMCID PMC3293912

    Pattern formation in developing tissues involves dynamic spatio-temporal changes in cellular organization and subsequent evolution of functional adult structures. Branching morphogenesis is a developmental mechanism by which patterns are generated in many developing organs, which is controlled b...
  19. A focal adhesion protein-based mechanochemical checkpoint regulates cleft progression during branching morphogenesis.

    Developmental Dynamics 240(9):2069 (2011) PMID 22016182 PMCID PMC3647453

    Cleft formation is the initial step of branching morphogenesis in many organs. We previously demonstrated that ROCK 1 regulates a nonmuscle myosin II-dependent mechanochemical checkpoint to transition initiated clefts to progressing clefts in developing submandibular salivary glands. Here, we re...
  20. Localization of AQP5 during development of the mouse submandibular salivary gland.

    Journal of Molecular Histology 42(1):71 (2011) PMID 21203896 PMCID PMC3063871

    Aquaporin 5 (AQP5) is known to be central for salivary fluid secretion. A study of the temporal-spatial distribution of AQP5 during submandibular gland (SMG) development and in adult tissues might offer further clues to its unknown role during development. In the present work, SMGs from embryoni...