1. The contribution of specific cell subpopulations to submandibular salivary gland branching morphogenesis

    Current Opinion in Genetics & Development 32:47 (2015)

    Branching morphogenesis is the developmental program responsible for generating a large surface to volume ratio in many secretory and absorptive organs. To accomplish branching morphogenesis, spatiotemporal regulation of specific cell subpopulations is required. Here, we review recent ...
  2. The contribution of specific cell subpopulations to submandibular salivary gland branching morphogenesis

    Current Opinion in Genetics & Development 32:47 (2015)

    Branching morphogenesis is the developmental program responsible for generating a large surface to volume ratio in many secretory and absorptive organs. To accomplish branching morphogenesis, spatiotemporal regulation of specific cell subpopulations is required. Here, we review recent ...
  3. The contribution of specific cell subpopulations to submandibular salivary gland branching morphogenesis

    Current Opinion in Genetics & Development 32:47 (2015)

    Branching morphogenesis is the developmental program responsible for generating a large surface to volume ratio in many secretory and absorptive organs. To accomplish branching morphogenesis, spatiotemporal regulation of specific cell subpopulations is required. Here, we review recent ...
  4. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis.

    Molecular Biology of the Cell 25(16):2393 (2014) PMID 24966172 PMCID PMC4142612

    Coordinated actin microfilament and microtubule dynamics is required for salivary gland development, although the mechanisms by which they contribute to branching morphogenesis are not defined. Because LIM kinase (LIMK) regulates both actin and microtubule organization, we investigated the role ...
  5. Biocompatible tissue scaffold compliance promotes salivary gland morphogenesis and differentiation.

    Tissue Engineering, Part A: Tissue Engineering 20(11-12):1632 (2014) PMID 24410370 PMCID PMC4029047

    Substrate compliance is reported to alter cell phenotype, but little is known about the effects of compliance on cell development within the context of a complex tissue. In this study, we used 0.48 and 19.66 kPa polyacrylamide gels to test the effects of the substrate modulus on submandibular sa...
  6. Biocompatible tissue scaffold compliance promotes salivary gland morphogenesis and differentiation.

    Tissue Engineering, Part A: Tissue Engineering 20(11-12):1632 (2014) PMID 24410370 PMCID PMC4029047

    Substrate compliance is reported to alter cell phenotype, but little is known about the effects of compliance on cell development within the context of a complex tissue. In this study, we used 0.48 and 19.66 kPa polyacrylamide gels to test the effects of the substrate modulus on submandibular sa...
  7. Microfluidic platform for the elastic characterization of mouse submandibular glands by atomic force microscopy.

    Biosensors 4(1):18 (2014) PMID 25587408

    The ability to characterize the microscale mechanical properties of biological materials has the potential for great utility in the field of tissue engineering. The development and morphogenesis of mammalian tissues are known to be guided in part by mechanical stimuli received from the local env...
  8. Microfluidic platform for the elastic characterization of mouse submandibular glands by atomic force microscopy.

    Biosensors 4(1):18 (2014) PMID 25587408 PMCID PMC4264367

    The ability to characterize the microscale mechanical properties of biological materials has the potential for great utility in the field of tissue engineering. The development and morphogenesis of mammalian tissues are known to be guided in part by mechanical stimuli received from the local env...
  9. Integrins promote cytokinesis through the RSK signaling axis.

    Journal of Cell Science 127(Pt 3):534 (2014) PMID 24284076 PMCID PMC4007764

    Cytokinesis is the final stage in cell division. Although integrins can regulate cytokinesis, the mechanisms involved are not fully understood. In this study, we demonstrate that integrin-regulated ERK (extracellular signal-related kinase) and RSK (p90 ribosomal S6 kinase) signaling promotes suc...
  10. Integrins promote cytokinesis through the RSK signaling axis.

    Journal of Cell Science 127(Pt 3):534 (2014) PMID 24284076 PMCID PMC4007764

    Cytokinesis is the final stage in cell division. Although integrins can regulate cytokinesis, the mechanisms involved are not fully understood. In this study, we demonstrate that integrin-regulated ERK (extracellular signal-related kinase) and RSK (p90 ribosomal S6 kinase) signaling promotes suc...
  11. Integrins promote cytokinesis through the RSK signaling axis.

    Journal of Cell Science 127(Pt 3):534 (2014) PMID 24284076 PMCID PMC4007764

    Cytokinesis is the final stage in cell division. Although integrins can regulate cytokinesis, the mechanisms involved are not fully understood. In this study, we demonstrate that integrin-regulated ERK (extracellular signal-related kinase) and RSK (p90 ribosomal S6 kinase) signaling promotes suc...
  12. Salivary gland cell differentiation and organization on micropatterned PLGA nanofiber craters.

    Biomaterials 34(28):6773 (2013) PMID 23777914 PMCID PMC3755621

    There is a need for an artificial salivary gland as a long-term remedy for patients suffering from salivary hypofunction, a leading cause of chronic xerostomia (dry mouth). Current salivary gland tissue engineering approaches are limited in that they either lack sufficient physical cues and surf...
  13. Salivary gland cell differentiation and organization on micropatterned PLGA nanofiber craters

    Biomaterials 34(28):6773 (2013)

    There is a need for an artificial salivary gland as a long-term remedy for patients suffering from salivary hypofunction, a leading cause of chronic xerostomia (dry mouth). Current salivary gland tissue engineering approaches are limited in that they either lack sufficient physical cue...
  14. Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue.

    PNAS 110(29):11982 (2013) PMID 23818604 PMCID PMC3718135

    Limitations on the number of unique protein and DNA molecules that can be characterized microscopically in a single tissue specimen impede advances in understanding the biological basis of health and disease. Here we present a multiplexed fluorescence microscopy method (MxIF) for quantitative, s...
  15. Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation.

    Biology Open 2(5):439 (2013) PMID 23789091 PMCID PMC3654261

    Epithelial organ morphogenesis involves reciprocal interactions between epithelial and mesenchymal cell types to balance progenitor cell retention and expansion with cell differentiation for evolution of tissue architecture. Underlying submandibular salivary gland branching morphogenesis is the ...
  16. Cell-based multi-parametric model of cleft progression during submandibular salivary gland branching morphogenesis.

    PLoS computational biology 9(11):e1003319 (2013) PMID 24277996 PMCID PMC3836695

    Cleft formation during submandibular salivary gland branching morphogenesis is the critical step initiating the growth and development of the complex adult organ. Previous experimental studies indicated requirements for several epithelial cellular processes, such as proliferation, migration, cel...
  17. Genetic modification and recombination of salivary gland organ cultures.

    Journal of Visualized Experiments (71):e50060 (2013) PMID 23407326 PMCID PMC3582689

    Branching morphogenesis occurs during the development of many organs, and the embryonic mouse submandibular gland (SMG) is a classical model for the study of branching morphogenesis. In the developing SMG, this process involves iterative steps of epithelial bud and duct formation, to ultimately ...
  18. Genetic modification and recombination of salivary gland organ cultures.

    Journal of Visualized Experiments (71):e50060 (2013) PMID 23407326

    Branching morphogenesis occurs during the development of many organs, and the embryonic mouse submandibular gland (SMG) is a classical model for the study of branching morphogenesis. In the developing SMG, this process involves iterative steps of epithelial bud and duct formation, to ultimately ...
  19. Selective functionalization of nanofiber scaffolds to regulate salivary gland epithelial cell proliferation and polarity

    Biomaterials 33(33):8372 (2012)

    Epithelial cell types typically lose apicobasal polarity when cultured on 2D substrates, but apicobasal polarity is required for directional secretion by secretory cells, such as salivary gland acinar cells. We cultured salivary gland epithelial cells on poly(lactic-co-glycolic acid) (...
  20. Selective functionalization of nanofiber scaffolds to regulate salivary gland epithelial cell proliferation and polarity.

    Biomaterials 33(33):8372 (2012) PMID 22938763 PMCID PMC3491572

    Epithelial cell types typically lose apicobasal polarity when cultured on 2D substrates, but apicobasal polarity is required for directional secretion by secretory cells, such as salivary gland acinar cells. We cultured salivary gland epithelial cells on poly(lactic-co-glycolic acid) (PLGA) nano...