1. Ronald J. Konopka (1947–2015).

    Cell 161(2):187 (2015) PMID 26042238

  2. Ronald J. Konopka (1947–2015)

    Cell 161(2):187 (2015)

  3. We'll always have RNA.

    RNA 21(4):546 (2015) PMID 25780131 PMCID PMC4371273

  4. Genome-wide features of neuroendocrine regulation in Drosophila by the basic helix-loop-helix transcription factor DIMMED.

    Nucleic Acids Research 43(4):2199 (2015) PMID 25634895

    Neuroendocrine (NE) cells use large dense core vesicles (LDCVs) to traffic, process, store and secrete neuropeptide hormones through the regulated secretory pathway. The dimmed (DIMM) basic helix-loop-helix transcription factor of Drosophila controls the level of regulated secretory activity in ...
    PDF not found
  5. Genome-wide features of neuroendocrine regulation in Drosophila by the basic helix-loop-helix transcription factor DIMMED.

    Nucleic Acids Research 43(4):2199 (2015) PMID 25634895

    Neuroendocrine (NE) cells use large dense core vesicles (LDCVs) to traffic, process, store and secrete neuropeptide hormones through the regulated secretory pathway. The dimmed (DIMM) basic helix-loop-helix transcription factor of Drosophila controls the level of regulated secretory activity in ...
    PDF not found
  6. Genome-wide features of neuroendocrine regulation in Drosophila by the basic helix-loop-helix transcription factor DIMMED.

    Nucleic Acids Research 43(4):2199 (2015) PMID 25634895 PMCID PMC4344488

    Neuroendocrine (NE) cells use large dense core vesicles (LDCVs) to traffic, process, store and secrete neuropeptide hormones through the regulated secretory pathway. The dimmed (DIMM) basic helix-loop-helix transcription factor of Drosophila controls the level of regulated secretory activity in ...
  7. RNA-seq Profiling of Small Numbers of Drosophila Neurons.

    Methods in Enzymology 551:369 (2015) PMID 25662465

    Drosophila melanogaster has a robust circadian clock, which drives a rhythmic behavior pattern: locomotor activity increases in the morning shortly before lights on (M peak) and in the evening shortly before lights off (E peak). This pattern is controlled by ~75 pairs of circadian neurons in the...
  8. Clk post-transcriptional control denoises circadian transcription both temporally and spatially.

    Nature Communications 6:7056 (2015) PMID 25952406

    The transcription factor CLOCK (CLK) is essential for the development and maintenance of circadian rhythms in Drosophila. However, little is known about how CLK levels are controlled. Here we show that Clk mRNA is strongly regulated post-transcriptionally through its 3' UTR. Flies expressing Clk...
  9. RNA-seq Profiling of Small Numbers of Drosophila Neurons.

    Methods in Enzymology 551:369 (2015) PMID 25662465

    Drosophila melanogaster has a robust circadian clock, which drives a rhythmic behavior pattern: locomotor activity increases in the morning shortly before lights on (M peak) and in the evening shortly before lights off (E peak). This pattern is controlled by ~75 pairs of circadian neurons in the...
  10. PDF and cAMP enhance PER stability in Drosophila clock neurons.

    PNAS 111(13):E1284 (2014) PMID 24707054 PMCID PMC3977231

    The neuropeptide PDF is important for Drosophila circadian rhythms: pdf(01) (pdf-null) animals are mostly arrhythmic or short period in constant darkness and have an advanced activity peak in light-dark conditions. PDF contributes to the amplitude, synchrony, as well as the pace of circadian rhy...
  11. PDF and cAMP enhance PER stability in Drosophila clock neurons.

    PNAS 111(13):E1284 (2014) PMID 24707054 PMCID PMC3977231

    The neuropeptide PDF is important for Drosophila circadian rhythms: pdf(01) (pdf-null) animals are mostly arrhythmic or short period in constant darkness and have an advanced activity peak in light-dark conditions. PDF contributes to the amplitude, synchrony, as well as the pace of circadian rhy...
  12. PDF neuron firing phase-shifts key circadian activity neurons in Drosophila.

    eLife 3 (2014) PMID 24939987 PMCID PMC4092873

    Our experiments address two long-standing models for the function of the Drosophila brain circadian network: a dual oscillator model, which emphasizes the primacy of PDF-containing neurons, and a cell-autonomous model for circadian phase adjustment. We identify five different circadian (E) neuro...
  13. CLOCK:BMAL1 is a pioneer-like transcription factor.

    Genes & Development 28(1):8 (2014) PMID 24395244 PMCID PMC3894415

    The mammalian circadian clock relies on the master genes CLOCK and BMAL1 to drive rhythmic gene expression and regulate biological functions under circadian control. Here we show that rhythmic CLOCK:BMAL1 DNA binding promotes rhythmic chromatin opening. Mechanisms include CLOCK:BMAL1 binding to ...
  14. CLOCK:BMAL1 is a pioneer-like transcription factor.

    Genes & Development 28(1):8 (2014) PMID 24395244 PMCID PMC3894415

    The mammalian circadian clock relies on the master genes CLOCK and BMAL1 to drive rhythmic gene expression and regulate biological functions under circadian control. Here we show that rhythmic CLOCK:BMAL1 DNA binding promotes rhythmic chromatin opening. Mechanisms include CLOCK:BMAL1 binding to ...
  15. PDF neuron firing phase-shifts key circadian activity neurons in Drosophila.

    eLife 3 (2014) PMID 24939987 PMCID PMC4092873

    Our experiments address two long-standing models for the function of the Drosophila brain circadian network: a dual oscillator model, which emphasizes the primacy of PDF-containing neurons, and a cell-autonomous model for circadian phase adjustment. We identify five different circadian (E) neuro...
  16. Short neuropeptide F is a sleep-promoting inhibitory modulator.

    Neuron 80(1):171 (2013) PMID 24094110 PMCID PMC3792499

    To advance the understanding of sleep regulation, we screened for sleep-promoting cells and identified neurons expressing neuropeptide Y-like short neuropeptide F (sNPF). Sleep induction by sNPF meets all relevant criteria. Rebound sleep following sleep deprivation is reduced by activation of sN...
  17. Short neuropeptide F is a sleep-promoting inhibitory modulator.

    Neuron 80(1):171 (2013) PMID 24094110 PMCID PMC3792499

    To advance the understanding of sleep regulation, we screened for sleep-promoting cells and identified neurons expressing neuropeptide Y-like short neuropeptide F (sNPF). Sleep induction by sNPF meets all relevant criteria. Rebound sleep following sleep deprivation is reduced by activation of sN...
  18. Short Neuropeptide F Is a Sleep-Promoting Inhibitory Modulator

    Neuron 80(1):171 (2013)

    To advance the understanding of sleep regulation, we screened for sleep-promoting cells and identified neurons expressing neuropeptide Y-like short neuropeptide F (sNPF). Sleep induction by sNPF meets all relevant criteria. Rebound sleep following sleep deprivation is reduced by activa...
  19. The transcription factor Mef2 links the Drosophila core clock to Fas2, neuronal morphology, and circadian behavior.

    Neuron 79(2):281 (2013) PMID 23889933 PMCID PMC3859024

    The transcription factor Mef2 regulates activity-dependent neuronal plasticity and morphology in mammals, and clock neurons are reported to experience activity-dependent circadian remodeling in Drosophila. We show here that Mef2 is required for this daily fasciculation-defasciculation cycle. Mor...
  20. The Transcription Factor Mef2 Links theDrosophilaCore Clock toFas2,Neuronal Morphology, and Circadian Behavior

    Neuron 79(2):281 (2013)

    The transcription factor Mef2 regulates activity-dependent neuronal plasticity and morphology in mammals, and clock neurons are reported to experience activity-dependent circadian remodeling in Drosophila. We show here that Mef2 is required for this daily fasciculation-defasciculation ...