1. UDP-galactose transporter gene hUGT1 expression in tobacco plants leads to hyper-galactosylated cell wall components.

    Journal of Bioscience and Bioengineering 121(5):573 (2016) PMID 26507776

    We reported previously that tobacco plants transformed with the human UDP-galactose transporter 1 gene (hUGT1-transgenic plants) displayed morphological, architectural, and physiological alterations, such as enhanced growth, increased accumulation of chlorophyll and lignin, and a gibberellin-res...
  2. Multidimensional High-Resolution Magic Angle Spinning and Solution-State NMR Characterization of (13)C-labeled Plant Metabolites and Lignocellulose.

    Scientific reports 5:11848 (2015) PMID 26143886 PMCID PMC4491710

    Lignocellulose, which includes mainly cellulose, hemicellulose, and lignin, is a potential resource for the production of chemicals and for other applications. For effective production of materials derived from biomass, it is important to characterize the metabolites and polymeric components of ...
  3. Metabolic flux analysis of genetically engineered Saccharomyces cerevisiae that produces lactate under micro-aerobic conditions.

    Bioprocess and Biosystems Engineering 36(9):1261 (2013) PMID 23223911

    In the present study, to elucidate mechanisms of growth suppression in YIBO-pdc1/5Δ, we performed carbon metabolic flux analysis under micro-aerobic conditions. Our results indicate that growth suppression of YIBO-pdc1/5Δ is caused by decreased flux to the pentose phosphate pathway, which suppli...
  4. Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass.

    Bioresource Technology 135:103 (2013) PMID 22850175

    The potential of 1-hexylpyridinium chloride ([Hpy][Cl]), to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose (Avicel) and Bagasse at 80 °C or 100 °C. Short [Hpy][Cl] pretreatments, <30 min, at lower temperature accelerate subsequent enzymatic saccharification of A...
  5. Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae.

    Journal of Bioscience and Bioengineering 115(5):467 (2013) PMID 23290995

    To create strains that have high productivity of lactic acid without neutralization, a genome-wide screening for strains showing hyper-resistance to 6% l-lactic acid (pH 2.6) was performed using the gene deletion collection of Saccharomyces cerevisiae. We identified 94 genes whose disruption led...
  6. Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter.

    Journal of Bioscience and Bioengineering 115(2):193 (2013) PMID 23021912

    A simple fed-batch system for cultivating genetically engineered yeast generating lactate under the regulation of the PDC1 promoter was established. Traditional strategies that avoid occurrence of Crabtree effect, such as respiratory quotient (RQ) control or ethanol control, are not applicable t...
  7. Amino acid residues important for CMP-sialic acid recognition by the CMP-sialic acid transporter: analysis of the substrate specificity of UDP-galactose/CMP-sialic acid transporter chimeras.

    Glycobiology 22(12):1731 (2012) PMID 22833315

    In our previous studies, we demonstrated that chimeric molecules of the CMP-sialic acid (CMP-Sia) transporter (CST) and the UDP-galactose (Gal) transporter (UGT) in which the seventh transmembrane helix-containing segment was derived from the CST could transport both CMP-Sia and UDP-Gal and that...
  8. Exploring the conformational space of amorphous cellulose using NMR chemical shifts.

    Carbohydrate polymers 90(3):1197 (2012) PMID 22939331

    (13)C-labeled amorphous cellulose and (13)C NMR chemical shifts by 2D (13)C-(13)C correlation spectroscopy were obtained in the regenerated solid-state from ionic liquids. On the basis of the assigned chemical shifts, combined with information from molecular dynamics and quantum chemistry comput...
  9. Investigation of utilization of the algal biomass residue after oil extraction to lower the total production cost of biodiesel.

    Journal of Bioscience and Bioengineering 114(3):330 (2012) PMID 22627052

    In this study, component analysis of a novel biodiesel-producing alga, Pseudochoricystis ellipsoidea, was performed. The component analysis results indicated that proteins and amino acids are abundant in P. ellipsoidea while the sugar content is relatively low. Rather than its use as a carbon so...
  10. Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide.

    Journal of Bioscience and Bioengineering 113(2):160 (2012) PMID 22036073

    We report that several tryptophan-rich peptides exhibit an affinity for a hydrophobic ionic liquid (IL) (1-ethyl-3-methylimidazolium bis-trifluoromethanesulfonyl imide), and that green fluorescent protein (GFP) fused to a peptides, "SSSWWSWWWW" (SW1) or "SWWWWSWWWW" (SW2), containing serine (S) ...
  11. Short time ionic liquids pretreatment on lignocellulosic biomass to enhance enzymatic saccharification

    Bioresource Technology 103(1):446 (2012) PMID 22033371

    Highlights ► We pretreated biomasses using [Bmpy][Cl], 1-buthyl-3-methylpyridinium chloride. ► Bagasse and Eucalyptus were pretreated at 120°C for 10min or 1h. ► The pretreatment caused cellulose conversion 3- to 8-fold higher than untreated ones. ► Short time pretreatment wi...
  12. Enhancement of cellulolytic enzyme activity by clustering cellulose binding domains on nanoscaffolds.

    Small 7(5):656 (2011) PMID 21290602

    Cellulose, one of the most abundant carbon resources, is degraded by cellulolytic enzymes called cellulases. Cellulases are generally modular proteins with independent catalytic and cellulose-binding domain (CBD) modules and, in some bacteria, catalytic modules are noncovalently assembled on a s...
  13. pH-uncontrolled lactic acid fermentation with activated carbon as an adsorbent

    Enzyme and Microbial Technology 48(6):526 (2011) PMID 22113026

    In this paper, we presented a novel process involving activated carbon (AC) as an adsorbent for lactic acid fermentation, separation and oligomerization. It was found that pH has a significant effect on the adsorption of lactic acid on AC. The use of AC for in situ removal of lactic acid su...
  14. Screening of optimal cellulases from symbiotic protists of termites through expression in the secretory pathway of Saccharomyces cerevisiae.

    Bioscience, Biotechnology, and Biochemistry 75(11):2260 (2011) PMID 22056456

    For direct and efficient ethanol production from cellulosic materials, we screened optimal cellulases from symbiotic protists of termites through heterologous expression with Saccharomyces cerevisiae. 11 cellulases, belonging to glycoside hydrolase families 5, 7, and 45 endoglucanases (EGs), wer...
  15. pH-uncontrolled lactic acid fermentation with activated carbon as an adsorbent

    Enzyme and Microbial Technology 48(6):526 (2011) PMID 22113026

    In this paper, we presented a novel process involving activated carbon (AC) as an adsorbent for lactic acid fermentation, separation and oligomerization. It was found that pH has a significant effect on the adsorption of lactic acid on AC. The use of AC for in situ removal of lactic acid su...
  16. Characterization of rice nucleotide sugar transporters capable of transporting UDP-galactose and UDP-glucose.

    Journal of Biochemistry 148(1):35 (2010) PMID 20305274

    Using the basic local alignment search tool (BLAST) algorithm to search the Oryza sativa (Japanese rice) nucleotide sequence databases with the Arabidopsis thaliana UDP-galactose transporter sequences as queries, we found a number of sequences encoding putative O. sativa UDP-galactose transporte...
  17. Promotion of efficient Saccharification of crystalline cellulose by Aspergillus fumigatus Swo1.

    Applied and Environmental Microbiology 76(8):2556 (2010) PMID 20173066 PMCID PMC2849193

    Swollenin is a protein from Trichoderma reesei that has a unique activity for disrupting cellulosic materials, and it has sequence similarity to expansins, plant cell wall proteins that have a loosening effect that leads to cell wall enlargement. In this study we cloned a gene encoding a swollen...
  18. Two pathways for importing GDP-fucose into the endoplasmic reticulum lumen function redundantly in the O-fucosylation of Notch in Drosophila.

    Journal of Biological Chemistry 285(6):4122 (2010) PMID 19948734 PMCID PMC2823552

    Notch is a transmembrane receptor that shares homology with proteins containing epidermal growth factor-like repeats and mediates the cell-cell interactions necessary for many cell fate decisions. In Drosophila, O-fucosyltransferase 1 catalyzes the O-fucosylation of these epidermal growth factor...
  19. The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants.

    Journal of Bioscience and Bioengineering 109(2):159 (2010) PMID 20129101

    When the human UDP-galactose transporter 1 gene (hUGT1) was introduced into tobacco plants, the plants displayed enhanced growth during cultivation, and axillary shoots had an altered determinate growth habit, elongating beyond the primary shoots and having a sympodial growth pattern similar to ...
  20. Analysis of CMP-sialic acid transporter-like proteins in plants.

    Phytochemistry 70(17-18):1973 (2009) PMID 19822337

    It is commonly accepted that sialic acids do not exist in plants. However, putative gene homologs of animal sialyltransferases and CMP-sialic acid transporters have been detected in the genomes of some plants. To elucidate the physiological functions of these genes, we cloned 2 cDNAs from Oryza ...