1. Tunicamycin-induced inhibition of protein secretion into culture medium of Arabidopsis T87 suspension cells through mRNA degradation on the endoplasmic reticulum.

    Bioscience, Biotechnology, and Biochemistry 80(6):1168 (2016) PMID 26923805

    The N-glycosylation inhibitor tunicamycin triggers endoplasmic reticulum stress response and inhibits efficient protein secretion in eukaryotes. Using Arabidopsis suspension cells, we showed that the reduced secretion of mannose-binding lectin 1 (MBL1) protein by tunicamycin is accompanied by a ...
  2. Arabidopsis tRNA ligase completes the cytoplasmic splicing of bZIP60 mRNA in the unfolded protein response.

    Biochemical and Biophysical Research Communicat... 470(4):941 (2016) PMID 26820526

    Arabidopsis bZIP60 is a major transcription factor that activates the unfolded protein response and is regulated by cytoplasmic splicing. Two Arabidopsis inositol-requiring 1s (IRE1A and IRE1B) cleave bZIP60 mRNA; however, the ligase that connects the two half-molecules of the split bZIP60 mRNA ...
  3. CaMV-35S promoter sequence-specific DNA methylation in lettuce.

    Plant Cell Reports 35(1):43 (2016) PMID 26373653

    We found 35S promoter sequence-specific DNA methylation in lettuce. Additionally, transgenic lettuce plants having a modified 35S promoter lost methylation, suggesting the modified sequence is subjected to the methylation machinery. We previously reported that cauliflower mosaic virus 35S promot...
  4. Exogenous salicylic acid activates two signaling arms of the unfolded protein response in Arabidopsis.

    Plant and Cell Physiology 55(10):1772 (2014) PMID 25138441

    The unfolded protein response (UPR) is a highly conserved cellular response that prevents abnormal maturation of proteins in the endoplasmic reticulum (ER). The expression of genes encoding ER chaperones is induced during the UPR. In the Arabidopsis UPR, two membrane-bound transcription factors,...
  5. The polar auxin transport inhibitor TIBA inhibits endoreduplication in dark grown spinach hypocotyls.

    Plant Science 225:45 (2014) PMID 25017158

    We addressed the question of whether an additional round of endoreduplication in dark-grown hypocotyls is a common feature in dicotyledonous plants having endopolyploid tissues. Ploidy distributions of hypocotyl tissues derived from in vitro-grown spinach (Spinacia oleracea L. cv. Atlas) seedlin...
  6. [Conservation and divergence of cytoplasmic splicing in the organisms].

    Seikagaku. The Journal of Japanese Biochemical ... 85(12):1095 (2013) PMID 24505943

  7. Defects in IRE1 enhance cell death and fail to degrade mRNAs encoding secretory pathway proteins in the Arabidopsis unfolded protein response.

    PNAS 110(14):5713 (2013) PMID 23509268 PMCID PMC3619347

    The unfolded protein response (UPR) is a cellular response highly conserved in eukaryotes to obviate accumulation of misfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring enzyme 1 (IRE1) catalyzes the cytoplasmic splicing of mRNA encoding bZIP transcription factors to activate...
  8. An attempt to detect siRNA-mediated genomic DNA modification by artificially induced mismatch siRNA in Arabidopsis.

    PLoS ONE 8(11):e81326 (2013) PMID 24278423 PMCID PMC3837478

    Although tremendous progress has been made in recent years in identifying molecular mechanisms of small interfering RNA (siRNA) functions in higher plants, the possibility of direct interaction between genomic DNA and siRNA remains an enigma. Such an interaction was proposed in the 'RNA cache' h...
  9. Plant transducers of the endoplasmic reticulum unfolded protein response.

    Trends in Plant Science 17(12):720 (2012) PMID 22796463

    The unfolded protein response (UPR) activates a set of genes to overcome accumulation of unfolded proteins in the endoplasmic reticulum (ER), a condition termed ER stress, and constitutes an essential part of ER protein quality control that ensures efficient maturation of secretory and membrane ...
  10. Analysis of a transcription factor using transient assay in Arabidopsis protoplasts.

    Methods in Molecular Biology 754:107 (2011) PMID 21720949

    Regulation of gene expression by transcription factors is a fundamental mechanism in essentially all aspects of cellular processes. Transient expression assay of a reporter plasmid containing a reporter gene driven by a promoter of interest and an effector plasmid expressing a transcription fact...
  11. Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor.

    Scientific reports 1:29 (2011) PMID 22355548 PMCID PMC3216516

    IRE1 plays an essential role in the endoplasmic reticulum (ER) stress response in yeast and mammals. We found that a double mutant of Arabidopsis IRE1A and IRE1B (ire1a/ire1b) is more sensitive to the ER stress inducer tunicamycin than the wild-type. Transcriptome analysis revealed that genes wh...
  12. Development of a series of gateway binary vectors possessing a tunicamycin resistance gene as a marker for the transformation of Arabidopsis thaliana.

    Bioscience, Biotechnology, and Biochemistry 75(4):804 (2011) PMID 21512216

    We made two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing a UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene driven by the nopaline synthase promoter (Pnos) as a tunicamycin resistance marker for the transformation of Arabidopsis thalia...
  13. Characterization of a plant-specific gene induced by endoplasmic reticulum stress in Arabidopsis thaliana.

    Bioscience, Biotechnology, and Biochemistry 74(10):2087 (2010) PMID 20944397

    We have conducted transcriptome analysis and have identified a number of genes that are upregulated by treatment with the endoplasmic reticulum (ER) stress inducer tunicamycin. Here we focused on one particular gene of unknown function. This gene, designated tunicamycin induced 1 (TIN1), encodes...
  14. Characteristics of the nuclear form of the Arabidopsis transcription factor AtbZIP60 during the endoplasmic reticulum stress response.

    Bioscience, Biotechnology, and Biochemistry 73(4):865 (2009) PMID 19352029

    Accumulation of unfolded proteins in the endoplasmic reticulum (ER) of eukaryotic cells triggers the transcriptional induction of ER-resident molecular chaperones to maintain cellular homeostasis, termed the ER stress response. Previously we isolated AtbZIP60, a membrane-bound transcription fact...
  15. Construction of a binary vector for transformation of Arabidopsis thaliana with a new selection marker.

    Bioscience, Biotechnology, and Biochemistry 72(11):3041 (2008) PMID 18997421

    A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation...
  16. NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in Nicotiana tabacum.

    Journal of Plant Research 121(6):603 (2008) PMID 18758894

    A spermine-based signal transduction pathway plays a defensive role against incompatible pathogens. We identified a novel spermine-responsive cDNA from Nicotiana tabacum that encodes a basic region/leucine zipper protein with a putative transmembrane domain. Identity to Arabidopsis thaliana AtbZ...
  17. Identification of an Arabidopsis transmembrane bZIP transcription factor involved in the endoplasmic reticulum stress response.

    Biochemical and Biophysical Research Communicat... 374(2):242 (2008) PMID 18634751

    Among 75 bZIP transcription factors identified in Arabidopsis, 3 (AtbZIP17, AtbZIP28, and AtbZIP49) possess a putative transmembrane domain (TMD) in addition to AtbZIP60, which was characterized previously. In the present study, cDNAs of AtbZIP17 and AtbZIP28 were isolated. Truncated forms of At...
  18. Identification of an Arabidopsis transmembrane bZIP transcription factor involved in the endoplasmic reticulum stress response.

    Biochemical and Biophysical Research Communicat... 374(2):242 (2008) PMID 18634751

    Among 75 bZIP transcription factors identified in Arabidopsis, 3 (AtbZIP17, AtbZIP28, and AtbZIP49) possess a putative transmembrane domain (TMD) in addition to AtbZIP60, which was characterized previously. In the present study, cDNAs of AtbZIP17 and AtbZIP28 were isolated. Truncated forms of At...
  19. The crystal structure of plant-specific calcium-binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14.

    Journal of Molecular Biology 377(1):246 (2008) PMID 18237745

    Calcium signals mediate a multitude of plant responses to external stimuli. Calcineurin B-like (CBL) proteins and their target kinases, CBL-interacting protein kinases (CIPKs), represent important relays in plant calcium signaling. CBL interacts with CIPK through a conserved motif (NAF/FISL moti...
  20. Identification of anArabidopsistransmembrane bZIP transcription factor involved in the endoplasmic reticulum stress response

    Biochemical and Biophysical Research Communicat... 374(2):242 (2008) PMID 18634751

    Among 75 bZIP transcription factors identified in Arabidopsis, 3 (AtbZIP17, AtbZIP28, and AtbZIP49) possess a putative transmembrane domain (TMD) in addition to AtbZIP60, which was characterized previously. In the present study, cDNAs of AtbZIP17 and AtbZIP28 were isolated. Truncated...