1. A BioBrick compatible strategy for genetic modification of plants.

    Journal of Biological Engineering 6(1):8 (2012) PMID 22716313 PMCID PMC3537565

    Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making plant engineering more widely accessible to bioengineers who can contribute diverse cre...
  2. Small molecule activators of SIRT1 as therapeutics for the treatment of type 2 diabetes.

    Nature 450(7170):712 (2007) PMID 18046409 PMCID PMC2753457

    Calorie restriction extends lifespan and produces a metabolic profile desirable for treating diseases of ageing such as type 2 diabetes. SIRT1, an NAD+-dependent deacetylase, is a principal modulator of pathways downstream of calorie restriction that produce beneficial effects on glucose homeost...
  3. MSN2 and MSN4 link calorie restriction and TOR to sirtuin-mediated lifespan extension in Saccharomyces cerevisiae.

    PLoS Biology 5(10):e261 (2007) PMID 17914901 PMCID PMC1994990

    Calorie restriction (CR) robustly extends the lifespan of numerous species. In the yeast Saccharomyces cerevisiae, CR has been proposed to extend lifespan by boosting the activity of sirtuin deacetylases, thereby suppressing the formation of toxic repetitive ribosomal DNA (rDNA) circles. An alte...
  4. Caloric restriction and life span determination of yeast cells.

    Methods in Molecular Biology 371:97 (2007) PMID 17634577

    The diet known as caloric restriction (CR) has been known for 70 yr to extend the life span of rodents (1). CR can also extend life span in a broad range of other species as well, from unicellular organisms (2,3), to invertebrates (4) and most likely primates, as well (5). The budding yeast Sacc...
  5. HST2 mediates SIR2-independent life-span extension by calorie restriction.

    Science 309(5742):1861 (2005) PMID 16051752

    Calorie restriction (CR) extends the life span of numerous species, from yeast to rodents. Yeast Sir2 is a nicotinamide adenine dinucleotide (NAD+-dependent histone deacetylase that has been proposed to mediate the effects of CR. However, this hypothesis has been challenged by the observation th...
  6. Yeast life-span extension by calorie restriction is independent of NAD fluctuation.

    Science 302(5653):2124 (2003) PMID 14605207

    Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected...
  7. Longevity regulation in Saccharomyces cerevisiae: linking metabolism, genome stability, and heterochromatin.

    Microbiology and Molecular Biology Reviews 67(3):376 (2003) PMID 12966141 PMCID PMC193872

    When it was first proposed that the budding yeast Saccharomyces cerevisiae might serve as a model for human aging in 1959, the suggestion was met with considerable skepticism. Although yeast had proved a valuable model for understanding basic cellular processes in humans, it was difficult to acc...
  8. Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae.

    Nature 423(6936):181 (2003) PMID 12736687

    Calorie restriction extends lifespan in a broad range of organisms, from yeasts to mammals. Numerous hypotheses have been proposed to explain this phenomenon, including decreased oxidative damage and altered energy metabolism. In Saccharomyces cerevisiae, lifespan extension by calorie restrictio...
  9. Manipulation of a nuclear NAD+ salvage pathway delays aging without altering steady-state NAD+ levels.

    Journal of Biological Chemistry 277(21):18881 (2002) PMID 11884393

    Yeast deprived of nutrients exhibit a marked life span extension that requires the activity of the NAD(+)-dependent histone deacetylase, Sir2p. Here we show that increased dosage of NPT1, encoding a nicotinate phosphoribosyltransferase critical for the NAD(+) salvage pathway, increases Sir2-depe...
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