An S-Acylation Switch of Conserved G Domain Cysteines Is Required for Polarity Signaling by ROP GTPases
Current Biology 25(21):2875 (2015)
Rac-mediated Stimulation of Phospholipase Cγ2 Amplifies B Cell Receptor-induced Calcium Signaling.
Journal of Biological Chemistry 290(28):17056 (2015)
The Rho GTPase Rac is crucially involved in controlling multiple B cell functions, including those regulated by the B cell receptor (BCR) through increased cytosolic Ca(2+). The underlying molecular mechanisms and their relevance to the functions of intact B cells have thus far remained unknown....
Src-mediated caveolin-1 phosphorylation affects the targeting of active Src to specific membrane sites.
Molecular Biology of the Cell 24(24):3881 (2013)
Src interactions with the plasma membrane are an important determinant of its activity. In turn, Src activity modulates its association with the membrane through binding of activated Src to phosphotyrosylated proteins. Caveolin-1 (Cav-1), a major component of caveolae, is a known Src phosphoryla...
Oligomeric interactions of TGF-β and BMP receptors
FEBS Letters 586(14):1885 (2012)
Transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP) cytokines participate in a multiplicity of ways in the regulation of numerous physiological and pathological processes. Their wide-ranging biological functions are controlled by several mechanisms, including regu...
Oligomeric interactions of TGF-β and BMP receptors.
FEBS Letters 586(14):1885 (2012)
Transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP) cytokines participate in a multiplicity of ways in the regulation of numerous physiological and pathological processes. Their wide-ranging biological functions are controlled by several mechanisms, including regulation of ...
Differential effects of prenylation and s-acylation on type I and II ROPS membrane interaction and function.
Plant Physiology 155(2):706 (2011)
Prenylation primarily by geranylgeranylation is required for membrane attachment and function of type I Rho of Plants (ROPs) and Gγ proteins, while type II ROPs are attached to the plasma membrane by S-acylation. Yet, it is not known how prenylation affects ROP membrane interaction dynamics and ...
An S-acylation switch of conserved G domain cysteines is required for polarity signaling by ROP GTPases.
Current Biology 20(10):914 (2010)
Rho GTPases are master regulators of cell polarity. For their function, Rhos must associate with discrete plasma membrane domains. Rho of Plants (ROPs) or RACs comprise a single family. Prenylation and S-acylation of hypervariable domain cysteines of Ras and Rho GTPases are required for their fu...
Differential regulation of phospholipase C-beta2 activity and membrane interaction by Galphaq, Gbeta1gamma2, and Rac2.
Journal of Biological Chemistry 285(6):3905 (2010)
We combined fluorescence recovery after photobleaching (FRAP) beam-size analysis with biochemical assays to investigate the mechanisms of membrane recruitment and activation of phospholipase C-beta(2) (PLCbeta(2)) by G protein alpha(q) and betagamma dimers. We show that activation by alpha(q) an...
DOC2B acts as a calcium switch and enhances vesicle fusion.
Journal of Neuroscience 28(27):6794 (2008)
Calcium-dependent exocytosis is regulated by a vast number of proteins. DOC2B is a synaptic protein that translocates to the plasma membrane (PM) after small elevations in intracellular calcium concentration. The aim of this study was to investigate the role of DOC2B in calcium-triggered exocyto...
Src kinase activity and SH2 domain regulate the dynamics of Src association with lipid and protein targets.
Journal of Cell Biology 178(4):675 (2007)
Src functions depend on its association with the plasma membrane and with specific membrane-associated assemblies. Many aspects of these interactions are unclear. We investigated the functions of kinase, SH2, and SH3 domains in Src membrane interactions. We used FRAP beam-size analysis in live c...
Cyclodextrins but not compactin inhibit the lateral diffusion of membrane proteins independent of cholesterol.
Traffic 7(7):917 (2006)
Cholesterol and glycosphingolipid-enriched membrane domains, termed lipid rafts, were proposed to play important roles in trafficking and signaling events. These functions are inhibited following putative disruption of rafts by cholesterol depletion, commonly induced by treatment with methyl-bet...
Rac2 regulation of phospholipase C-beta 2 activity and mode of membrane interactions in intact cells.
Journal of Biological Chemistry 278(10):8645 (2003)
Phospholipase C-beta (PLCbeta) isozymes play important roles in transmembrane signaling. Their activity is regulated by heterotrimeric G proteins. The PLCbeta(2) isozyme is unique in being stimulated also by Rho GTPases (Rac and Cdc42). However, the mechanism(s) of this stimulation are still unc...
Activated K-Ras and H-Ras display different interactions with saturable nonraft sites at the surface of live cells.
Journal of Cell Biology 157(5):865 (2002)
Ras-membrane interactions play important roles in signaling and oncogenesis. H-Ras and K-Ras have nonidentical membrane anchoring moieties that can direct them to different membrane compartments. Ras-lipid raft interactions were reported, but recent studies suggest that activated K-Ras and H-Ras...
Interaction of Sendai virions with resealed human erythrocyte ghosts Lateral mobility of the viral glycoproteins in the cell membrane following fusion
FEBS Letters 228(2):281 (1988)
Two independent methods demonstrated that resealed human erythrocyte ghosts undergo Sendai virus-mediated cell-cell fusion to a much lower degree (about 4%) than intact erythrocytes, in spite of similar levels of viral envelope-cell fusion in the two preparations. Fluorescence photobleachin...
Lateral diffusion and patch formation of H-2Kkantigens on mouse spleen lymphocytes
Biochimica et Biophysica Acta (BBA) - Molecular... 762(2):281 (1983)
We have studied the diffusion and aggregation of H-2K
k antigens labeled with a fluorescent anti-H-2K
k monoclonal antibody (IgG) on mouse splenic lymphocytes, employing fluorescence photobleaching recovery and fluorescence microscopy. The H-2K
k antigens were initially di...