1. Rad54 is required for the normal development of male and female germ cells and contributes to the maintainance of their genome integrity after genotoxic stress.

    Cell Death and Disease 4:e774 (2013) PMID 23949223 PMCID PMC3763443

    Rad54 is an important factor in the homologous recombination pathway of DNA double-strand break repair. However, Rad54 knockout (KO) mice do not exhibit overt phenotypes at adulthood, even when exposed to radiation. In this study, we show that in Rad54 KO mouse the germline is actually altered. ...
  2. Rad54 is required for the normal development of male and female germ cells and contributes to the maintainance of their genome integrity after genotoxic stress.

    Cell Death and Disease 4:e774 (2013) PMID 23949223 PMCID PMC3763443

    Rad54 is an important factor in the homologous recombination pathway of DNA double-strand break repair. However, Rad54 knockout (KO) mice do not exhibit overt phenotypes at adulthood, even when exposed to radiation. In this study, we show that in Rad54 KO mouse the germline is actually altered. ...
  3. Chromatin mobility is increased at sites of DNA double-strand breaks.

    Journal of Cell Science 125(Pt 9):2127 (2012) PMID 22328517

    DNA double-strand breaks (DSBs) can efficiently kill cancer cells, but they can also produce unwanted chromosome rearrangements when DNA ends from different DSBs are erroneously joined. Movement of DSB-containing chromatin domains might facilitate these DSB interactions and promote the formation...
  4. Chromatin mobility is increased at sites of DNA double-strand breaks.

    Journal of Cell Science 125(Pt 9):2127 (2012) PMID 22328517

    DNA double-strand breaks (DSBs) can efficiently kill cancer cells, but they can also produce unwanted chromosome rearrangements when DNA ends from different DSBs are erroneously joined. Movement of DSB-containing chromatin domains might facilitate these DSB interactions and promote the formation...
  5. Opposite modifying effects of HR and NHEJ deficiency on cancer risk in Ptc1 heterozygous mouse cerebellum.

    Oncogene 30(47):4740 (2011) PMID 21602895

    Heterozygous Patched1 (Ptc1(+/-)) mice are prone to medulloblastoma (MB), and exposure of newborn mice to ionizing radiation dramatically increases the frequency and shortens the latency of MB. In Ptc1(+/-) mice, MB is characterized by loss of the normal remaining Ptc1 allele, suggesting that ge...
  6. Opposite modifying effects of HR and NHEJ deficiency on cancer risk in Ptc1 heterozygous mouse cerebellum.

    Oncogene 30(47):4740 (2011) PMID 21602895

    Heterozygous Patched1 (Ptc1(+/-)) mice are prone to medulloblastoma (MB), and exposure of newborn mice to ionizing radiation dramatically increases the frequency and shortens the latency of MB. In Ptc1(+/-) mice, MB is characterized by loss of the normal remaining Ptc1 allele, suggesting that ge...
  7. Effect of the BRCA2 CTRD domain on RAD51 filaments analyzed by an ensemble of single molecule techniques.

    Nucleic Acids Research 39(15):6558 (2011) PMID 21576230 PMCID PMC3159462

    Homologous recombination is essential for the preservation of genome stability, thereby preventing cancer. The recombination protein RAD51 drives DNA strand exchange, which requires the assembly, rearrangement and disassembly of a RAD51 filament on DNA, coupled to ATP binding and hydrolysis. Thi...
  8. Effect of the BRCA2 CTRD domain on RAD51 filaments analyzed by an ensemble of single molecule techniques.

    Nucleic Acids Research 39(15):6558 (2011) PMID 21576230 PMCID PMC3159462

    Homologous recombination is essential for the preservation of genome stability, thereby preventing cancer. The recombination protein RAD51 drives DNA strand exchange, which requires the assembly, rearrangement and disassembly of a RAD51 filament on DNA, coupled to ATP binding and hydrolysis. Thi...
  9. Visualizing RAD51-mediated joint molecules: implications for recombination mechanism and the effect of sequence heterology.

    Nucleic Acids Research 39(1):155 (2011) PMID 20817928 PMCID PMC3017611

    The defining event in homologous recombination is the exchange of base-paired partners between a single-stranded (ss) DNA and a homologous duplex driven by recombinase proteins, such as human RAD51. To understand the mechanism of this essential genome maintenance event, we analyzed the structure...
  10. Resolving RAD51 Filament Nucleation, Extension and Disassembly on ssDNA and dsDNA One Protein at a Time

    Biophysical Journal 100(3):240a (2011)

  11. Visualizing RAD51-mediated joint molecules: implications for recombination mechanism and the effect of sequence heterology.

    Nucleic Acids Research 39(1):155 (2011) PMID 20817928 PMCID PMC3017611

    The defining event in homologous recombination is the exchange of base-paired partners between a single-stranded (ss) DNA and a homologous duplex driven by recombinase proteins, such as human RAD51. To understand the mechanism of this essential genome maintenance event, we analyzed the structure...
  12. BLM has early and late functions in homologous recombination repair in mouse embryonic stem cells.

    Oncogene 29(33):4705 (2010) PMID 20531307

    BLM is a RecQ family helicase that is defective in individuals with the cancer predisposition disorder, Bloom's syndrome (BS). At the cellular level, BS is characterized by hyper-recombination manifested as excessive sister chromatid exchange and loss of heterozygosity. However, the precise func...
  13. BLM has early and late functions in homologous recombination repair in mouse embryonic stem cells.

    Oncogene 29(33):4705 (2010) PMID 20531307

    BLM is a RecQ family helicase that is defective in individuals with the cancer predisposition disorder, Bloom's syndrome (BS). At the cellular level, BS is characterized by hyper-recombination manifested as excessive sister chromatid exchange and loss of heterozygosity. However, the precise func...
  14. Disruption of maternal DNA repair increases sperm-derived chromosomal aberrations.

    PNAS 104(45):17725 (2007) PMID 17978187 PMCID PMC2077046

    Male and female germ cells can transmit genetic defects that lead to pregnancy loss, infant mortality, birth defects, and genetic diseases in offspring; however, the parental origins of transmitted defects are not random, with de novo mutations and chromosomal structural aberrations transmitted ...
  15. Disruption of maternal DNA repair increases sperm-derived chromosomal aberrations.

    PNAS 104(45):17725 (2007) PMID 17978187 PMCID PMC2077046

    Male and female germ cells can transmit genetic defects that lead to pregnancy loss, infant mortality, birth defects, and genetic diseases in offspring; however, the parental origins of transmitted defects are not random, with de novo mutations and chromosomal structural aberrations transmitted ...
  16. Effects of radiation on cultured mucosal substitues

    Journal of Plastic, Reconstructive & Aesthetic ... 60(4):S15 (2007)

  17. The structure-specific endonuclease Ercc1-Xpf is required for targeted gene replacement in embryonic stem cells.

    EMBO Journal 20(22):6540 (2001) PMID 11707424 PMCID PMC125716

    The Ercc1-Xpf heterodimer, a highly conserved structure-specific endonuclease, functions in multiple DNA repair pathways that are pivotal for maintaining genome stability, including nucleotide excision repair, interstrand crosslink repair and homologous recombination. Ercc1-Xpf incises double-st...
  18. The structure-specific endonuclease Ercc1-Xpf is required for targeted gene replacement in embryonic stem cells.

    EMBO Journal 20(22):6540 (2001) PMID 11707424 PMCID PMC125716

    The Ercc1-Xpf heterodimer, a highly conserved structure-specific endonuclease, functions in multiple DNA repair pathways that are pivotal for maintaining genome stability, including nucleotide excision repair, interstrand crosslink repair and homologous recombination. Ercc1-Xpf incises double-st...
  19. Human Rad50/Mre11 is a flexible complex that can tether DNA ends.

    Molecular Cell 8(5):1129 (2001) PMID 11741547

    The human Rad50 protein, classified as a structural maintenance of chromosomes (SMC) family member, is complexed with Mre11 (R/M) and has important functions in at least two distinct double-strand break repair pathways. To find out what the common function of R/M in these pathways might be, we i...
  20. Human Rad50/Mre11 is a flexible complex that can tether DNA ends.

    Molecular Cell 8(5):1129 (2001) PMID 11741547

    The human Rad50 protein, classified as a structural maintenance of chromosomes (SMC) family member, is complexed with Mre11 (R/M) and has important functions in at least two distinct double-strand break repair pathways. To find out what the common function of R/M in these pathways might be, we i...