1. Glypican-1 identifies cancer exosomes and detects early pancreatic cancer.

    Nature 523(7559):177 (2015) PMID 26106858

    Exosomes are lipid-bilayer-enclosed extracellular vesicles that contain proteins and nucleic acids. They are secreted by all cells and circulate in the blood. Specific detection and isolation of cancer-cell-derived exosomes in the circulation is currently lacking. Using mass spectrometry analyse...
  2. miR-21 Inhibition Reduces Liver Fibrosis and Prevents Tumor Development by Inducing Apoptosis of CD24+ Progenitor Cells.

    Cancer Research 75(9):1859 (2015) PMID 25769721

    miR-21 is upregulated in hepatocellular carcinoma and intrahepatic cholangiocarcinoma, where it is associated with poor prognosis. Here, we offer preclinical evidence that miR-21 offers a therapeutic and chemopreventive target in these liver cancers. In mice with hepatic deletion of Pten, anti-m...
  3. Epigenetic balance of aberrant Rasal1 promoter methylation and hydroxymethylation regulates cardiac fibrosis.

    Cardiovascular Research 105(3):279 (2015) PMID 25616414

    Methylation of CpG island promoters is a prototypical epigenetic mechanism to stably control gene expression. The aim of this study was to elucidate the contribution of aberrant promoter DNA methylation in pathological endothelial to mesenchymal transition (EndMT) and subsequent cardiac fibrosis...
  4. Epigenetic balance of aberrant Rasal1 promoter methylation and hydroxymethylation regulates cardiac fibrosis.

    Cardiovascular Research 105(3):279 (2015) PMID 25616414

    Methylation of CpG island promoters is a prototypical epigenetic mechanism to stably control gene expression. The aim of this study was to elucidate the contribution of aberrant promoter DNA methylation in pathological endothelial to mesenchymal transition (EndMT) and subsequent cardiac fibrosis...
  5. Endocardial fibroelastosis is caused by aberrant endothelial to mesenchymal transition.

    Circulation Research 116(5):857 (2015) PMID 25587097 PMCID PMC4344885

    Endocardial fibroelastosis (EFE) is a unique form of fibrosis, which forms a de novo subendocardial tissue layer encapsulating the myocardium and stunting its growth, and which is typically associated with congenital heart diseases of heterogeneous origin, such as hypoplastic left heart syndrome...
  6. Endocardial fibroelastosis is caused by aberrant endothelial to mesenchymal transition.

    Circulation Research 116(5):857 (2015) PMID 25587097 PMCID PMC4344885

    Endocardial fibroelastosis (EFE) is a unique form of fibrosis, which forms a de novo subendocardial tissue layer encapsulating the myocardium and stunting its growth, and which is typically associated with congenital heart diseases of heterogeneous origin, such as hypoplastic left heart syndrome...
  7. Endocardial fibroelastosis is caused by aberrant endothelial to mesenchymal transition.

    Circulation Research 116(5):857 (2015) PMID 25587097

    Endocardial fibroelastosis (EFE) is a unique form of fibrosis, which forms a de novo subendocardial tissue layer encapsulating the myocardium and stunting its growth, and which is typically associated with congenital heart diseases of heterogeneous origin, such as hypoplastic left heart syndrome...
  8. Endocardial fibroelastosis is caused by aberrant endothelial to mesenchymal transition.

    Circulation Research 116(5):857 (2015) PMID 25587097

    Endocardial fibroelastosis (EFE) is a unique form of fibrosis, which forms a de novo subendocardial tissue layer encapsulating the myocardium and stunting its growth, and which is typically associated with congenital heart diseases of heterogeneous origin, such as hypoplastic left heart syndrome...
  9. Targeting Vascular Pericytes in Hypoxic Tumors Increases Lung Metastasis via Angiopoietin-2.

    Cell Reports 10(7):1066 (2015) PMID 25704811 PMCID PMC4342328

    Strategies to target angiogenesis include inhibition of the vessel-stabilizing properties of vascular pericytes. Pericyte depletion in early-stage non-hypoxic tumors suppressed nascent angiogenesis, tumor growth, and lung metastasis. In contrast, pericyte depletion in advanced-stage hypoxic tumo...
  10. Targeting Vascular Pericytes in Hypoxic Tumors Increases Lung Metastasis via Angiopoietin-2

    Cell Reports 10(7):1066 (2015)

    Strategies to target angiogenesis include inhibition of the vessel-stabilizing properties of vascular pericytes. Pericyte depletion in early-stage non-hypoxic tumors suppressed nascent angiogenesis, tumor growth, and lung metastasis. In contrast, pericyte depletion in advanced-stage hy...
  11. Targeting Vascular Pericytes in Hypoxic Tumors Increases Lung Metastasis via Angiopoietin-2.

    Cell Reports 10(7):1066 (2015) PMID 25704811 PMCID PMC4342328

    Strategies to target angiogenesis include inhibition of the vessel-stabilizing properties of vascular pericytes. Pericyte depletion in early-stage non-hypoxic tumors suppressed nascent angiogenesis, tumor growth, and lung metastasis. In contrast, pericyte depletion in advanced-stage hypoxic tumo...
  12. Targeting Vascular Pericytes in Hypoxic Tumors Increases Lung Metastasis via Angiopoietin-2.

    Cell Reports 10(7):1066 (2015) PMID 25704811

    Strategies to target angiogenesis include inhibition of the vessel-stabilizing properties of vascular pericytes. Pericyte depletion in early-stage non-hypoxic tumors suppressed nascent angiogenesis, tumor growth, and lung metastasis. In contrast, pericyte depletion in advanced-stage hypoxic tumo...
  13. Targeting vascular pericytes in hypoxic tumors increases lung metastasis via angiopoietin-2.

    Cell Reports 10(7):1066 (2015) PMID 25704811 PMCID PMC4342328

    Strategies to target angiogenesis include inhibition of the vessel-stabilizing properties of vascular pericytes. Pericyte depletion in early-stage non-hypoxic tumors suppressed nascent angiogenesis, tumor growth, and lung metastasis. In contrast, pericyte depletion in advanced-stage hypoxic tumo...
  14. Induction of Tet3-dependent Epigenetic Remodeling by Low-dose Hydralazine Attenuates Progression of Chronic Kidney Disease

    EBioMedicine 2(1):19 (2015)

    Progression of chronic kidney disease remains a principal problem in clinical nephrology and there is a pressing need for novel therapeutics and biomarkers. Aberrant promoter CpG island methylation and subsequent transcriptional silencing of specific genes have emerged as contributors ...
  15. Genome-wide profiling of p53-regulated enhancer RNAs uncovers a subset of enhancers controlled by a lncRNA.

    Nature Communications 6:6520 (2015) PMID 25813522 PMCID PMC4389233

    p53 binds enhancers to regulate key target genes. Here, we globally mapped p53-regulated enhancers by looking at enhancer RNA (eRNA) production. Intriguingly, while many p53-induced enhancers contained p53-binding sites, most did not. As long non-coding RNAs (lncRNAs) are prominent regulators of...
  16. Cancer exosomes perform cell-independent microRNA biogenesis and promote tumorigenesis.

    Cancer Cell 26(5):707 (2014) PMID 25446899 PMCID PMC4254633

    Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC-Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into ...
  17. Cancer Exosomes Perform Cell-Independent MicroRNA Biogenesis and Promote Tumorigenesis.

    Cancer Cell 26(5):707 (2014) PMID 25446899 PMCID PMC4254633

    Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC-Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into ...
  18. Cancer Exosomes Perform Cell-Independent MicroRNA Biogenesis and Promote Tumorigenesis.

    Cancer Cell 26(5):707 (2014) PMID 25446899 PMCID PMC4254633

    Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC-Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into ...
  19. Cancer exosomes perform cell-independent microRNA biogenesis and promote tumorigenesis.

    Cancer Cell 26(5):707 (2014) PMID 25446899 PMCID PMC4254633

    Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC-Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into ...
  20. Cancer exosomes perform cell-independent microRNA biogenesis and promote tumorigenesis.

    Cancer Cell 26(5):707 (2014) PMID 25446899 PMCID PMC4254633

    Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC-Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into ...