1. α5 and αv integrins cooperate to regulate vascular smooth muscle and neural crest functions in vivo.

    Development 142(4):797 (2015) PMID 25670798

    The RGD-binding α5 and αv integrins have been shown to be key regulators of vascular smooth muscle cell (vSMC) function in vitro. However, their role on vSMCs during vascular development in vivo remains unclear. To address this issue, we have generated mice that lack α5, αv or both α5 and αv int...
  2. α5 and αv integrins cooperate to regulate vascular smooth muscle and neural crest functions in vivo.

    Development 142(4):797 (2015) PMID 25670798

    The RGD-binding α5 and αv integrins have been shown to be key regulators of vascular smooth muscle cell (vSMC) function in vitro. However, their role on vSMCs during vascular development in vivo remains unclear. To address this issue, we have generated mice that lack α5, αv or both α5 and αv int...
  3. Tumor angiogenesis in the absence of fibronectin or its cognate integrin receptors.

    PLoS ONE 10(3):e0120872 (2015) PMID 25807551 PMCID PMC4373772

    Binding of α5β1 and αvβ3/β5 integrin receptors on the endothelium to their fibronectin substrate in the extracellular matrix has been targeted as a possible means of blocking tumor angiogenesis and tumor growth. However, clinical trials of blocking antibodies and peptides have been disappointing...
  4. Stretching the boundaries of extracellular matrix research.

    Nature Reviews: Molecular Cell Biology 15(12):761 (2014) PMID 25574535

    Extracellular matrix (ECM) proteins constitute >1% of the proteome and interact with many modifiers and growth factors to affect most aspects of cellular behaviour during development and normal physiology, as well as in diseases such as fibroses, cancer and many genetic disorders. In addition to...
  5. Stretching the boundaries of extracellular matrix research.

    Nature Reviews: Molecular Cell Biology 15(12):761 (2014) PMID 25574535

    Extracellular matrix (ECM) proteins constitute >1% of the proteome and interact with many modifiers and growth factors to affect most aspects of cellular behaviour during development and normal physiology, as well as in diseases such as fibroses, cancer and many genetic disorders. In addition to...
  6. Alternative splicing of endothelial fibronectin is induced by disturbed hemodynamics and protects against hemorrhage of the vessel wall.

    Arteriosclerosis, Thrombosis, and Vascular Biology 34(9):2042 (2014) PMID 24903094 PMCID PMC4140979

    Abnormally low-flow conditions, sensed by the arterial endothelium, promote aneurysm rupture. Fibronectin (FN) is among the most abundant extracellular matrix proteins and is strongly upregulated in human aneurysms, suggesting a possible role in disease progression. Altered FN splicing can resul...
  7. Alternative splicing of endothelial fibronectin is induced by disturbed hemodynamics and protects against hemorrhage of the vessel wall.

    Arteriosclerosis, Thrombosis, and Vascular Biology 34(9):2042 (2014) PMID 24903094 PMCID PMC4140979

    Abnormally low-flow conditions, sensed by the arterial endothelium, promote aneurysm rupture. Fibronectin (FN) is among the most abundant extracellular matrix proteins and is strongly upregulated in human aneurysms, suggesting a possible role in disease progression. Altered FN splicing can resul...
  8. Integrin-α5β1 is not required for mural cell functions during development of blood vessels but is required for lymphatic-blood vessel separation and lymphovenous valve formation.

    Developmental Biology 392(2):381 (2014) PMID 24858485 PMCID PMC4113717

    Integrin α5β1 is essential for vascular development but it remains unclear precisely where and how it functions. Here, we report that deletion of the gene encoding the integrin-α5 subunit (Itga5) using the Pdgfrb-Cre transgenic mouse line, leads to oedema, haemorrhage and increased levels of emb...
  9. Platelets guide the formation of early metastatic niches.

    PNAS 111(30):E3053 (2014) PMID 25024172 PMCID PMC4121772

    During metastasis, host cells are recruited to disseminated tumor cells to form specialized microenvironments ("niches") that promote metastatic progression, but the mechanisms guiding the assembly of these niches are largely unknown. Tumor cells may autonomously recruit host cells or, alternati...
  10. Platelets guide the formation of early metastatic niches.

    PNAS 111(30):E3053 (2014) PMID 25024172 PMCID PMC4121772

    During metastasis, host cells are recruited to disseminated tumor cells to form specialized microenvironments ("niches") that promote metastatic progression, but the mechanisms guiding the assembly of these niches are largely unknown. Tumor cells may autonomously recruit host cells or, alternati...
  11. Extracellular matrix signatures of human mammary carcinoma identify novel metastasis promoters.

    eLife 3:e01308 (2014) PMID 24618895 PMCID PMC3944437

    The extracellular matrix (ECM) is a major component of tumors and a significant contributor to cancer progression. In this study, we use proteomics to investigate the ECM of human mammary carcinoma xenografts and show that primary tumors of differing metastatic potential differ in ECM compositio...
  12. Extracellular matrix signatures of human mammary carcinoma identify novel metastasis promoters.

    eLife 3:e01308 (2014) PMID 24618895 PMCID PMC3944437

    The extracellular matrix (ECM) is a major component of tumors and a significant contributor to cancer progression. In this study, we use proteomics to investigate the ECM of human mammary carcinoma xenografts and show that primary tumors of differing metastatic potential differ in ECM compositio...
  13. Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.

    BMC Cancer 14:518 (2014) PMID 25037231 PMCID PMC4223627

    Colorectal cancer is the third most frequently diagnosed cancer and the third cause of cancer deaths in the United States. Despite the fact that tumor cell-intrinsic mechanisms controlling colorectal carcinogenesis have been identified, novel prognostic and diagnostic tools as well as novel ther...
  14. Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.

    BMC Cancer 14:518 (2014) PMID 25037231 PMCID PMC4223627

    Colorectal cancer is the third most frequently diagnosed cancer and the third cause of cancer deaths in the United States. Despite the fact that tumor cell-intrinsic mechanisms controlling colorectal carcinogenesis have been identified, novel prognostic and diagnostic tools as well as novel ther...
  15. Determination of 35 cell surface antigen levels in malignant pleural effusions identifies CD24 as a marker of disseminated tumor cells.

    International Journal of Cancer 133(12):2925 (2013) PMID 23775727 PMCID PMC4107365

    Many targets have been identified in solid tumors for antibody therapy but it is less clear what surface antigens may be most commonly expressed on disseminated tumor cells. Using malignant pleural effusions as a source of disseminated tumor cells, we compared a panel of 35 antigens for their ca...
  16. Determination of 35 cell surface antigen levels in malignant pleural effusions identifies CD24 as a marker of disseminated tumor cells.

    International Journal of Cancer 133(12):2925 (2013) PMID 23775727 PMCID PMC4107365

    Many targets have been identified in solid tumors for antibody therapy but it is less clear what surface antigens may be most commonly expressed on disseminated tumor cells. Using malignant pleural effusions as a source of disseminated tumor cells, we compared a panel of 35 antigens for their ca...
  17. Determination of 35 cell surface antigen levels in malignant pleural effusions identifies CD24 as a marker of disseminated tumor cells.

    International Journal of Cancer 133(12):2925 (2013) PMID 23775727 PMCID PMC4107365

    Many targets have been identified in solid tumors for antibody therapy but it is less clear what surface antigens may be most commonly expressed on disseminated tumor cells. Using malignant pleural effusions as a source of disseminated tumor cells, we compared a panel of 35 antigens for their ca...
  18. Determination of 35 cell surface antigen levels in malignant pleural effusions identifies CD24 as a marker of disseminated tumor cells.

    International Journal of Cancer 133(12):2925 (2013) PMID 23775727 PMCID PMC4107365

    Many targets have been identified in solid tumors for antibody therapy but it is less clear what surface antigens may be most commonly expressed on disseminated tumor cells. Using malignant pleural effusions as a source of disseminated tumor cells, we compared a panel of 35 antigens for their ca...
  19. In Vivo RNAi Screening Identifies a Leukemia-Specific Dependence on Integrin Beta 3 Signaling

    Cancer Cell 24(1):45 (2013)

    We used an in vivo small hairpin RNA (shRNA) screening approach to identify genes that are essential for MLL-AF9 acute myeloid leukemia (AML). We found that Integrin Beta 3 (Itgb3) is essential for murine leukemia cells in vivo and for human leukemia cells in xenotransplantation studie...
  20. In Vivo RNAi screening identifies a leukemia-specific dependence on integrin beta 3 signaling.

    Cancer Cell 24(1):45 (2013) PMID 23770013 PMCID PMC3746037

    We used an in vivo small hairpin RNA (shRNA) screening approach to identify genes that are essential for MLL-AF9 acute myeloid leukemia (AML). We found that Integrin Beta 3 (Itgb3) is essential for murine leukemia cells in vivo and for human leukemia cells in xenotransplantation studies. In leuk...