1. Burkholderia pseudomallei type III secretion system cluster 3 ATPase BsaS, a chemotherapeutic target for small-molecule ATPase inhibitors.

    Infection and Immunity 83(4):1276 (2015) PMID 25605762 PMCID PMC4363454

    Melioidosis is an infectious disease of high mortality for humans and other animal species; it is prevalent in tropical regions worldwide. The pathogenesis of melioidosis depends on the ability of its causative agent, the Gram-negative bacterium Burkholderia pseudomallei, to enter and survive in...
  2. Autophagy and burkholderia.

    Immunology and Cell Biology 93(1):18 (2015) PMID 25331551

    Autophagy has become increasingly viewed as an important component of the eukaryotic innate immune system. The elimination of intracellular pathogens by autophagy in mammalian cells (xenophagy) results not only in the degradation of invading bacteria, viruses, fungi and parasites, but also liber...
  3. Autophagy and burkholderia.

    Immunology and Cell Biology 93(1):18 (2015) PMID 25331551

    Autophagy has become increasingly viewed as an important component of the eukaryotic innate immune system. The elimination of intracellular pathogens by autophagy in mammalian cells (xenophagy) results not only in the degradation of invading bacteria, viruses, fungi and parasites, but also liber...
  4. X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein.

    PLoS ONE 10(4):e0123338 (2015) PMID 25923520 PMCID PMC4414407

    Phanta is a reversibly photoswitching chromoprotein (ΦF, 0.003), useful for pcFRET, that was isolated from a mutagenesis screen of the bright green fluorescent eCGP123 (ΦF, 0.8). We have investigated the contribution of substitutions at positions His193, Thr69 and Gln62, individually and in comb...
  5. Autophagy in development, cell differentiation, and homeodynamics: from molecular mechanisms to diseases and pathophysiology.

    BioMed Research International 2014:349623 (2014) PMID 25247173 PMCID PMC4163348

  6. Autophagy in development, cell differentiation, and homeodynamics: from molecular mechanisms to diseases and pathophysiology.

    BioMed Research International 2014:349623 (2014) PMID 25247173 PMCID PMC4163348

  7. Evolutionary analysis of Burkholderia pseudomallei identifies putative novel virulence genes, including a microbial regulator of host cell autophagy.

    Journal of Bacteriology 195(24):5487 (2013) PMID 24097950 PMCID PMC3889600

    Burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with ∼2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these B. pseudomallei genes, which can be discer...
  8. Evolutionary analysis of Burkholderia pseudomallei identifies putative novel virulence genes, including a microbial regulator of host cell autophagy.

    Journal of Bacteriology 195(24):5487 (2013) PMID 24097950 PMCID PMC3889600

    Burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with ∼2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these B. pseudomallei genes, which can be discer...
  9. Nucleophagy at a glance.

    Journal of Cell Science 126(Pt 19):4325 (2013) PMID 24013549

    Under certain circumstances, the removal of damaged or non-essential parts of the nucleus, or even an entire nucleus, is crucial in order to promote cell longevity and enable proper function. A selective form of autophagy, known as nucleophagy, can be used to accomplish the degradation of nucleu...
  10. Nucleophagy at a glance.

    Journal of Cell Science 126(Pt 19):4325 (2013) PMID 24013549

    Under certain circumstances, the removal of damaged or non-essential parts of the nucleus, or even an entire nucleus, is crucial in order to promote cell longevity and enable proper function. A selective form of autophagy, known as nucleophagy, can be used to accomplish the degradation of nucleu...
  11. The impact of autophagic processes on the intracellular fate of Helicobacter pylori: more tricks from an enigmatic pathogen?

    Autophagy 9(5):639 (2013) PMID 23396129 PMCID PMC3669176

    Helicobacter pylori is a Gram-negative pathogen that colonizes the gastric epithelium of 50-60% of the world's population. Approximately one-fifth of the infected individuals manifest severe diseases such as peptic ulcers or gastric cancer. H. pylori infection has proven difficult to cure despit...
  12. The impact of autophagic processes on the intracellular fate of Helicobacter pylori: more tricks from an enigmatic pathogen?

    Autophagy 9(5):639 (2013) PMID 23396129 PMCID PMC3669176

    Helicobacter pylori is a Gram-negative pathogen that colonizes the gastric epithelium of 50-60% of the world's population. Approximately one-fifth of the infected individuals manifest severe diseases such as peptic ulcers or gastric cancer. H. pylori infection has proven difficult to cure despit...
  13. Beclin 1 is required for starvation-enhanced, but not rapamycin-enhanced, LC3-associated phagocytosis of Burkholderia pseudomallei in RAW 264.7 cells.

    Infection and Immunity 81(1):271 (2013) PMID 23115045 PMCID PMC3536138

    LC3-associated phagocytosis (LAP) of Burkholderia pseudomallei by murine macrophage (RAW 264.7) cells is an intracellular innate defense mechanism. Beclin 1, a protein with several roles in autophagic processes, is known to be recruited to phagosomal membranes as a very early event in LAP. We so...
  14. Beclin 1 is required for starvation-enhanced, but not rapamycin-enhanced, LC3-associated phagocytosis of Burkholderia pseudomallei in RAW 264.7 cells.

    Infection and Immunity 81(1):271 (2013) PMID 23115045 PMCID PMC3536138

    LC3-associated phagocytosis (LAP) of Burkholderia pseudomallei by murine macrophage (RAW 264.7) cells is an intracellular innate defense mechanism. Beclin 1, a protein with several roles in autophagic processes, is known to be recruited to phagosomal membranes as a very early event in LAP. We so...
  15. Phanta: a non-fluorescent photochromic acceptor for pcFRET.

    PLoS ONE 8(9):e75835 (2013) PMID 24098733 PMCID PMC3786930

    We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption sp...
  16. Phanta: a non-fluorescent photochromic acceptor for pcFRET.

    PLoS ONE 8(9):e75835 (2013) PMID 24098733 PMCID PMC3786930

    We have developed an orange non-fluorescent photochromic protein (quantum yield, 0.003) we call Phanta that is useful as an acceptor in pcFRET applications. Phanta can be repeatedly inter-converted between the two absorbing states by alternate exposure to cyan and violet light. The absorption sp...
  17. Transitory phases of autophagic death and programmed necrosis during superoxide-induced neuronal cell death

    Free Radical Biology and Medicine 53(10):1960 (2012)

    Neurons can undergo a diverse range of death responses under oxidative stress, encompassing apoptosis (caspase-dependent, programmed cell death) to various forms of caspase-independent death, including necrosis. We recently showed that primary murine cortical neurons exposed acutely to...
  18. Transitory phases of autophagic death and programmed necrosis during superoxide-induced neuronal cell death.

    Free Radical Biology and Medicine 53(10):1960 (2012) PMID 22982049

    Neurons can undergo a diverse range of death responses under oxidative stress, encompassing apoptosis (caspase-dependent, programmed cell death) to various forms of caspase-independent death, including necrosis. We recently showed that primary murine cortical neurons exposed acutely to hydrogen ...
  19. Transitory phases of autophagic death and programmed necrosis during superoxide-induced neuronal cell death.

    Free Radical Biology and Medicine 53(10):1960 (2012) PMID 22982049

    Neurons can undergo a diverse range of death responses under oxidative stress, encompassing apoptosis (caspase-dependent, programmed cell death) to various forms of caspase-independent death, including necrosis. We recently showed that primary murine cortical neurons exposed acutely to hydrogen ...
  20. Receptor protein complexes are in control of autophagy.

    Autophagy 8(11):1701 (2012) PMID 22874568 PMCID PMC3494607

    In autophagic processes a variety of cargos is delivered to the degradative compartment of cells. Recent progress in autophagy research has provided support for the notion that when autophagic processes are operating in selective mode, a receptor protein complex will process the cargo. Here we p...