1. Sustained Wnt/β-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole.

    Nature Communications 5:4168 (2014) PMID 24942669

    β-Catenin mediates the canonical Wnt pathway by stimulating Tcf-dependent transcription and also associates to N-cadherin at the apical complex (AC) of neuroblasts. Here, we show that while β-catenin activity is required to form the AC and to maintain the cell polarity, oncogenic mutations that ...
  2. Sustained Wnt/β-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole.

    Nature Communications 5:4168 (2014) PMID 24942669

    β-Catenin mediates the canonical Wnt pathway by stimulating Tcf-dependent transcription and also associates to N-cadherin at the apical complex (AC) of neuroblasts. Here, we show that while β-catenin activity is required to form the AC and to maintain the cell polarity, oncogenic mutations that ...
  3. Smad2 and Smad3 cooperate and antagonize simultaneously in vertebrate neurogenesis.

    Journal of Cell Science 126(Pt 23):5335 (2013) PMID 24105267

    The transforming growth factor beta (TGF-β) pathway plays key roles in development and cancer. TGF-β signaling converges on the Smad2 and Smad3 effectors, which can either cooperate or antagonize to regulate their transcriptional targets. Here we performed in vivo and in silico experiments to st...
  4. Smad2 and Smad3 cooperate and antagonize simultaneously in vertebrate neurogenesis.

    Journal of Cell Science 126(Pt 23):5335 (2013) PMID 24105267

    The transforming growth factor beta (TGF-β) pathway plays key roles in development and cancer. TGF-β signaling converges on the Smad2 and Smad3 effectors, which can either cooperate or antagonize to regulate their transcriptional targets. Here we performed in vivo and in silico experiments to st...
  5. MicroRNA 22 regulates cell cycle length in cerebellar granular neuron precursors.

    Molecular and Cellular Biology 33(14):2706 (2013) PMID 23671190 PMCID PMC3700129

    During cerebellum development, Sonic hedgehog (Shh)-induced proliferation of cerebellar granular neuronal precursors (CGNPs) is potently inhibited by bone morphogenetic proteins (BMPs). We have previously reported the upregulation of TIEG-1 and Mash1, two antimitotic factors that modulate MYCN t...
  6. MicroRNA 22 regulates cell cycle length in cerebellar granular neuron precursors.

    Molecular and Cellular Biology 33(14):2706 (2013) PMID 23671190 PMCID PMC3700129

    During cerebellum development, Sonic hedgehog (Shh)-induced proliferation of cerebellar granular neuronal precursors (CGNPs) is potently inhibited by bone morphogenetic proteins (BMPs). We have previously reported the upregulation of TIEG-1 and Mash1, two antimitotic factors that modulate MYCN t...
  7. IRS-2 Deficiency impairs NMDA receptor-dependent long-term potentiation.

    Cerebral Cortex 22(8):1717 (2012) PMID 21955917 PMCID PMC3388895

    The beneficial effects of insulin and insulin-like growth factor I on cognition have been documented in humans and animal models. Conversely, obesity, hyperinsulinemia, and diabetes increase the risk for neurodegenerative disorders including Alzheimer's disease (AD). However, the mechanisms by w...
  8. IRS-2 Deficiency impairs NMDA receptor-dependent long-term potentiation.

    Cerebral Cortex 22(8):1717 (2012) PMID 21955917 PMCID PMC3388895

    The beneficial effects of insulin and insulin-like growth factor I on cognition have been documented in humans and animal models. Conversely, obesity, hyperinsulinemia, and diabetes increase the risk for neurodegenerative disorders including Alzheimer's disease (AD). However, the mechanisms by w...
  9. Sonic Hedgehog-induced proliferation requires specific Gα inhibitory proteins.

    Journal of Biological Chemistry 286(10):8067 (2011) PMID 21209076 PMCID PMC3048693

    Proliferation of cerebellar granular neuronal precursors (CGNPs) is mediated by Sonic Hedgehog (Shh), which activates the Patched and Smoothened (Smo) receptor complex. Although its protein sequence suggests that Smo is a G protein coupled receptor (GPCR), the evidence that this receptor utilize...
  10. Sonic Hedgehog-induced proliferation requires specific Gα inhibitory proteins.

    Journal of Biological Chemistry 286(10):8067 (2011) PMID 21209076 PMCID PMC3048693

    Proliferation of cerebellar granular neuronal precursors (CGNPs) is mediated by Sonic Hedgehog (Shh), which activates the Patched and Smoothened (Smo) receptor complex. Although its protein sequence suggests that Smo is a G protein coupled receptor (GPCR), the evidence that this receptor utilize...
  11. Sonic-hedgehog-mediated proliferation requires the localization of PKA to the cilium base.

    Journal of Cell Science 123(Pt 1):62 (2010) PMID 20016067

    Cerebellar granular neuronal precursors (CGNPs) proliferate in response to the mitogenic activity of Sonic hedgehog (Shh), and this proliferation is negatively regulated by activation of cAMP-dependent protein kinase (PKA). In the basal state, the PKA catalytic subunits (C-PKA) are inactive beca...
  12. Sonic-hedgehog-mediated proliferation requires the localization of PKA to the cilium base.

    Journal of Cell Science 123(Pt 1):62 (2010) PMID 20016067

    Cerebellar granular neuronal precursors (CGNPs) proliferate in response to the mitogenic activity of Sonic hedgehog (Shh), and this proliferation is negatively regulated by activation of cAMP-dependent protein kinase (PKA). In the basal state, the PKA catalytic subunits (C-PKA) are inactive beca...
  13. Probucol increases glutathione peroxidase-1 activity and displays long-lasting protection against methylmercury toxicity in cerebellar granule cells.

    Toxicological Sciences 112(2):416 (2009) PMID 19770487

    Methylmercury (MeHg) is an environmental neurotoxicant whose molecular mechanisms underlying toxicity remain elusive. Here, we investigated molecular events involved in MeHg-induced neurotoxicity in cultured cerebellar granule cells (CGCs) as well as potential protective strategies for such toxi...
  14. Probucol increases glutathione peroxidase-1 activity and displays long-lasting protection against methylmercury toxicity in cerebellar granule cells.

    Toxicological Sciences 112(2):416 (2009) PMID 19770487

    Methylmercury (MeHg) is an environmental neurotoxicant whose molecular mechanisms underlying toxicity remain elusive. Here, we investigated molecular events involved in MeHg-induced neurotoxicity in cultured cerebellar granule cells (CGCs) as well as potential protective strategies for such toxi...
  15. Expression of the proneural gene encoding Mash1 suppresses MYCN mitotic activity.

    Journal of Cell Science 122(Pt 5):595 (2009) PMID 19208763

    Murine Mash1 (Ascl1) is a member of the basic helix-loop-helix family of transcription factors and has been described to promote differentiation in some neural precursors. The process of differentiation is coordinated with a concomitant cell-cycle arrest, but the molecular mechanism of this proc...
  16. Expression of the proneural gene encoding Mash1 suppresses MYCN mitotic activity.

    Journal of Cell Science 122(Pt 5):595 (2009) PMID 19208763

    Murine Mash1 (Ascl1) is a member of the basic helix-loop-helix family of transcription factors and has been described to promote differentiation in some neural precursors. The process of differentiation is coordinated with a concomitant cell-cycle arrest, but the molecular mechanism of this proc...
  17. Bone morphogenetic protein 2 opposes Shh-mediated proliferation in cerebellar granule cells through a TIEG-1-based regulation of Nmyc.

    Journal of Biological Chemistry 282(51):37170 (2007) PMID 17951258

    Nmyc is a potent regulator of cell cycle in cerebellar granular neuron precursors (CGNPs) and has been proposed to be the main effector of Shh (Sonic hedgehog) proliferative activity. Nmyc ectopic expression is sufficient to promote cell autonomous proliferation and can lead to tumorigenesis. Bo...
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  18. Bone morphogenetic protein 2 opposes Shh-mediated proliferation in cerebellar granule cells through a TIEG-1-based regulation of Nmyc.

    Journal of Biological Chemistry 282(51):37170 (2007) PMID 17951258

    Nmyc is a potent regulator of cell cycle in cerebellar granular neuron precursors (CGNPs) and has been proposed to be the main effector of Shh (Sonic hedgehog) proliferative activity. Nmyc ectopic expression is sufficient to promote cell autonomous proliferation and can lead to tumorigenesis. Bo...
    PDF not found
  19. Bmp2 antagonizes sonic hedgehog-mediated proliferation of cerebellar granule neurones through Smad5 signalling.

    Development 131(13):3159 (2004) PMID 15197161

    During development of the cerebellum, sonic hedgehog (Shh) is directly responsible for the proliferation of granule cell precursors in the external germinal layer. We have looked for signals able to regulate a switch from the Shh-mediated proliferative response to one that directs differentiatio...
  20. Bmp2 antagonizes sonic hedgehog-mediated proliferation of cerebellar granule neurones through Smad5 signalling.

    Development 131(13):3159 (2004) PMID 15197161

    During development of the cerebellum, sonic hedgehog (Shh) is directly responsible for the proliferation of granule cell precursors in the external germinal layer. We have looked for signals able to regulate a switch from the Shh-mediated proliferative response to one that directs differentiatio...