1. Use of non-invasive serum glycan markers to distinguish non-alcoholic steatohepatitis from simple steatosis.

    Journal of Gastroenterology and Hepatology 30(3):528 (2015) PMID 25168691

    Serum glycans have been reported to be promising diagnostic markers for many inflammatory diseases and cancers. The aims of this study were to investigate whole glycan expression in patients with non-alcoholic fatty liver diseases and to evaluate the potential use of glycan profiles as new clini...
  2. Application of glyco-blotting for identification of structures of polysaccharides causing membrane fouling in a pilot-scale membrane bioreactor treating municipal wastewater.

    Bioresource Technology 179:180 (2015) PMID 25544495

    A new approach for the analysis of polysaccharides in membrane bioreactor (MBR) is proposed in this study. Enrichment of polysaccharides by glyco-blotting, in which polysaccharides are specifically collected via interactions between the aldehydes in the polysaccharides and aminooxy groups on gly...
  3. Delineating Binding Modes of Gal/GalNAc and Structural Elements of the Molecular Recognition of Tumor-Associated Mucin Glycopeptides by the Human Macrophage Galactose-Type Lectin.

    Chemistry: A European Journal 20(49):16147 (2014) PMID 25324212

    The human macrophage galactose-type lectin (MGL) is a key physiological receptor for the carcinoma-associated Tn antigen (GalNAc-α-1-O-Ser/Thr) in mucins. NMR and modeling-based data on the molecular recognition features of synthetic Tn-bearing glycopeptides by MGL are presented. Cognate epitope...
  4. Delineating binding modes of Gal/GalNAc and structural elements of the molecular recognition of tumor-associated mucin glycopeptides by the human macrophage galactose-type lectin.

    Chemistry: A European Journal 20(49):16147 (2014) PMID 25324212

    The human macrophage galactose-type lectin (MGL) is a key physiological receptor for the carcinoma-associated Tn antigen (GalNAc-α-1-O-Ser/Thr) in mucins. NMR and modeling-based data on the molecular recognition features of synthetic Tn-bearing glycopeptides by MGL are presented. Cognate epitope...
  5. Fast epitope mapping for the anti-MUC1 monoclonal antibody by combining a one-bead-one-glycopeptide library and a microarray platform.

    Chemistry: A European Journal 20(48):15891 (2014) PMID 25303614

    Anti-MUC1 monoclonal antibodies (mAbs) are powerful tools that can be used to recognize cancer-related MUC1 molecules, the O-glycosylation status of which is believed to affect binding affinity. We demonstrate the feasibility of using a rapid screening methodology to elucidate those effects. The...
  6. Fast Epitope Mapping for the Anti-MUC1 Monoclonal Antibody by Combining a One-Bead-One-Glycopeptide Library and a Microarray Platform.

    Chemistry: A European Journal 20(48):15891 (2014) PMID 25303614

    Anti-MUC1 monoclonal antibodies (mAbs) are powerful tools that can be used to recognize cancer-related MUC1 molecules, the O-glycosylation status of which is believed to affect binding affinity. We demonstrate the feasibility of using a rapid screening methodology to elucidate those effects. The...
  7. Quantitative glycomics monitoring of induced pluripotent- and embryonic stem cells during neuronal differentiation.

    Stem Cell Research 13(3 Pt A):454 (2014) PMID 25460606

    Alterations in the structure of cell surface glycoforms occurring during the stages of stem cell differentiation remain unclear. We describe a rapid glycoblotting-based cellular glycomics method for quantitatively evaluating changes in glycoform expression and structure during neuronal different...
  8. Serum tri- and tetra-antennary N-glycan is a potential predictive biomarker for castration-resistant prostate cancer.

    Prostate 74(15):1521 (2014) PMID 25154914

    The U.S. FDA has approved several novel systemic agents including abiraterone acetate and taxoid cabazitaxel for metastatic castration-resistant prostate cancer (CRPC) result in a complicated decision-making while selecting an appropriate treatment. Therefore, a predictive biomarker for CRPC wou...
  9. Quantitative glycomics monitoring of induced pluripotent- and embryonic stem cells during neuronal differentiation.

    Stem Cell Research 13(3 Pt A):454 (2014) PMID 25460606

    Alterations in the structure of cell surface glycoforms occurring during the stages of stem cell differentiation remain unclear. We describe a rapid glycoblotting-based cellular glycomics method for quantitatively evaluating changes in glycoform expression and structure during neuronal different...
  10. Serum tri- and tetra-antennary N-glycan is a potential predictive biomarker for castration-resistant prostate cancer.

    Prostate 74(15):1521 (2014) PMID 25154914

    The U.S. FDA has approved several novel systemic agents including abiraterone acetate and taxoid cabazitaxel for metastatic castration-resistant prostate cancer (CRPC) result in a complicated decision-making while selecting an appropriate treatment. Therefore, a predictive biomarker for CRPC wou...
  11. Quantitative glycomics monitoring of induced pluripotent- and embryonic stem cells during neuronal differentiation

    Stem Cell Research 13(3):454 (2014)

    Alterations in the structure of cell surface glycoforms occurring during the stages of stem cell differentiation remain unclear. We describe a rapid glycoblotting-based cellular glycomics method for quantitatively evaluating changes in glycoform expression and structure during neuronal...
  12. A comparison of N-glycan profiles in human plasma and vitreous fluid.

    Graefe's Archive for Clinical and Experimental ... 252(8):1235 (2014) PMID 24912590

    To investigate the concentration and composition of N-glycans in plasma and vitreous samples obtained from patients with non-proliferative vitreoretinal diseases. Plasma and vitreous samples were collected from 11 patients with idiopathic macular hole (MH) and 9 patients with epiretinal membrane...
  13. A comparison of N-glycan profiles in human plasma and vitreous fluid.

    Graefe's Archive for Clinical and Experimental ... 252(8):1235 (2014) PMID 24912590

    To investigate the concentration and composition of N-glycans in plasma and vitreous samples obtained from patients with non-proliferative vitreoretinal diseases. Plasma and vitreous samples were collected from 11 patients with idiopathic macular hole (MH) and 9 patients with epiretinal membrane...
  14. MP39-04 N- AND O-GLYCOME ANALYSIS OF SERUM AND URINE FROM BLADDER CANCER PATIENTS USING A HIGH–THROUGHPUT GLYCOBLOTTING METHOD

    The Journal of Urology 191(4):e427 (2014)

  15. A straightforward protocol for the preparation of high performance microarray displaying synthetic MUC1 glycopeptides

    Biochimica et Biophysica Acta (BBA) - General S... 1840(3):1105 (2014)

    Background Human serum MUC1 peptide fragments bearing aberrant O-glycans are secreted from columnar epithelial cell surfaces and known as clinically important serum biomarkers for the epithelial carcinoma when a specific monoclonal antibody can probe disease-relevant epitopes...
  16. Serum N-glycan alteration associated with renal cell carcinoma detected by high throughput glycan analysis.

    The Journal of Urology 191(3):805 (2014) PMID 24140550

    Biomarkers for the early detection and prediction of survival in patients with renal cell carcinoma have not been established. We developed what is to our knowledge a novel glycoblotting method that allows high throughput, comprehensive, quantitative analysis of glycans in human serum. In this s...
  17. SerumN-Glycan Alteration Associated with Renal Cell Carcinoma Detected by High Throughput Glycan Analysis

    The Journal of Urology 191(3):805 (2014)

    Purpose Biomarkers for the early detection and prediction of survival in patients with renal cell carcinoma have not been established. We developed what is to our knowledge a novel glycoblotting method that allows high throughput, comprehensive, quantitative analysis of glyca...
  18. A straightforward protocol for the preparation of high performance microarray displaying synthetic MUC1 glycopeptides.

    Biochimica et Biophysica Acta 1840(3):1105 (2014) PMID 24246952

    Human serum MUC1 peptide fragments bearing aberrant O-glycans are secreted from columnar epithelial cell surfaces and known as clinically important serum biomarkers for the epithelial carcinoma when a specific monoclonal antibody can probe disease-relevant epitopes. Despite the growing importanc...
  19. Synthesis of neoglycosphingolipid from methoxyamino-functionalized ceramide

    Bioorganic & Medicinal Chemistry Letters 24(4):1197 (2014)

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  20. Synthesis of neoglycosphingolipid from methoxyamino-functionalized ceramide.

    Bioorganic & Medicinal Chemistry Letters 24(4):1197 (2014) PMID 24461288

    An efficient approach for the synthesis of a methoxyamino-functionalized ceramide was established from phytosphingosine using specific Nβ→Nα acyl migration of the octadecanoyl group during the removal of Nα-Fmoc protective group. One step glycoblotting reaction of the ceramide mimic with lactose...