1. Environmentally benign process for the preparation of antimicrobial α-methylene-β-hydroxy-γ-butyrolactone (tulipalin B) from tulip biomass.

    Bioscience, Biotechnology, and Biochemistry 79(1):25 (2015) PMID 25126881

    Tulipalin B (α-methylene-β-hydroxy-γ-butyrolactone, PaB) is an antimicrobial natural product occurring in tulip (Tulipa gesneriana). PaB is directly formed from the precursor glucose ester 6-tuliposide B (PosB) by endogenous Pos-converting enzyme (TCE). Despite the potential usefulness of antiba...
  2. Two new β-glucosidases from ethanol-fermenting fungus Mucor circinelloides NBRC 4572: enzyme purification, functional characterization, and molecular cloning of the gene.

    Applied Microbiology and Biotechnology 97(23):10045 (2013) PMID 24061417

    Two β-glucosidases (BGLs 1 and 2) were purified to homogeneity from the extracellular enzyme preparations of the ethanol-fermenting Mucor circinelloides NBRC 4572 statically grown on rice straw. BGLs 1 and 2 are monomeric glycoproteins whose apparent molecular masses (Ms) are around 78 kDa, whic...
  3. Molecular diversity of tuliposide A-converting enzyme in the tulip.

    Bioscience, Biotechnology, and Biochemistry 77(5):1042 (2013) PMID 23649245

    Tuliposide A-converting enzyme (TCEA) catalyzes the conversion of 6-tuliposide A to its lactonized aglycon, tulipalin A, in the tulip (Tulipa gesneriana). The TgTCEA gene, isolated previously from petals, was transcribed in all tulip tissues but not in the bulbs despite the presence of TCEA acti...
  4. A novel lactone-forming carboxylesterase: molecular identification of a tuliposide A-converting enzyme in tulip.

    Plant Physiology 159(2):565 (2012) PMID 22474185 PMCID PMC3375925

    Tuliposides, the glucose esters of 4-hydroxy-2-methylenebutanoate and 3,4-dihydroxy-2-methylenebutanoate, are major secondary metabolites in tulip (Tulipa gesneriana). Their lactonized aglycons, tulipalins, function as defensive chemicals due to their biological activities. We recently found tha...
  5. A novel xylogenic suspension culture model for exploring lignification in Phyllostachys bamboo.

    Plant Methods 8(1):40 (2012) PMID 22978662 PMCID PMC3462127

    Some prominent cultured plant cell lines, such as the BY-2 cell line of tobacco (Nicotiana tabacum cv. 'Bright Yellow 2') and the T87 cell line of Arabidopsis (Arabidopsis thaliana L. Heynh., ecotype Columbia) are used as model plant cells. These suspension cell culture systems are highly applic...
  6. Methyl jasmonate-induced enhancement of expression activity of Am-FaPS-1, a putative farnesyl diphosphate synthase gene from Aquilaria microcarpa.

    Journal of Natural Medicines 65(1):194 (2011) PMID 20686864

    A cDNA clone, designated Am-FaPS-1 (1310 bp), was isolated from callus culture derived from the leaf tissues of Aquilaria microcarpa. This gene contains an open reading frame encoding the protein of 342 amino acid residues with high homology to farnesyl diphosphate synthase from various plant so...
  7. Development of bioethanol production by consolidated bioprocess usingM.javanicus

    Journal of Biotechnology 150:158 (2010)

  8. Distribution and biosynthesis of theanine in Theaceae plants.

    Plant Physiology and Biochemistry 48(1):70 (2010) PMID 19828327

    The theanine content of the leaves of 27 species or varieties of Theaceae plants was investigated. Theanine was present in 21 species or varieties, but in much lower amounts (<0.2 mumol/g fresh weight) than the quantity detected in Camellia sinensis var. sinensis. The major free amino acids in l...
  9. Transcriptional activation of putative calmodulin genes am-cam-1 and am-cam-2 from Aquilaria microcarpa, in response to external stimuli.

    Biological & Pharmaceutical Bulletin 33(11):1911 (2010) PMID 21048321

    A homology-based cloning strategy yielded two cDNA clones designated Am-cam-1 and Am-cam-2, presumably encoding calmodulin protein from a callus culture derived from the leaf tissues of Aquilaria microcarpa. An appreciable increase in the transcriptional activity of Am-cam-1 was reproducibly obs...
  10. Distribution and biosynthesis of theanine in Theaceae plants

    Plant Physiology and Biochemistry 48(1):70 (2010)

    The theanine content of the leaves of 27 species or varieties of Theaceae plants was investigated. Theanine was present in 21 species or varieties, but in much lower amounts (<0.2 μmol/g fresh weight) than the quantity detected in Camellia sinensis var. sinensis. The major free amino...
  11. Purification and characterization of a tuliposide-converting enzyme from bulbs of Tulipa gesneriana.

    Bioscience, Biotechnology, and Biochemistry 73(8):1895 (2009) PMID 19661715

    An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana. The enzyme appeared to be a dimer, the relative molecular mass (Mr) of each subunit being 34,900; it had maximum activity and stability at neutral...
  12. A simple shoot multiplication procedure using internode explants, and its application for particle bombardment and Agrobacterium-mediated transformation in watercress.

    Journal of Plant Research 122(4):455 (2009) PMID 19308313

    A shoot multiplication system derived from internode explants was investigated with the aim of improving genetic characteristics of watercress (Nasturtium officinale R. Br.). Internodes of ca. 1 cm excised from in vitro stock shoot culture were placed on half-strength Murashige and Skoog (MS) me...
  13. Possibility for selective accumulation of polyphenolics in tissue cultures of senno (Lychnis senno Siebold et Zucc.).

    Natural product communications 4(3):377 (2009) PMID 19413116

    Senno (Lychnis senno Siebold et Zucc.), a traditional ornamental plant in Japan had been used as a crude drug acting as natural blood thinners. Since tissue culture protocols have been established, we analyzed polyphenol accumulation profiles in shoot culture, multiple shoot culture, and callus ...
  14. A facile method for the preparation of α-methylene-γ-butyrolactones from tulip tissues by enzyme-mediated conversion

    Tetrahedron Letters 50(33):4751 (2009)

    A facile preparation of α-methylene-γ-butyrolactone (tulipalin) by enzyme-mediated conversion of 6-tuliposide and selective extraction has been developed.
  15. Biosynthesis of theanine (γ-ethylamino-l-glutamic acid) in seedlings ofCamellia sinensis

    Phytochemistry Letters 1(2):115 (2008)

    Theanine is a major amino acid in young tea seedlings. It is synthesised from [ 15N]glutamic acid, [ 15N]alanine and [ 14C]ethylamine in all parts of tea seedlings. Conversion of exogenously supplied ( 15NH 4) 2SO 4 is utilised in theanine synthesis...
  16. Fine control of caffeine biosynthesis in tissue cultures ofCamellia sinensis

    Phytochemistry Letters 1(4):195 (2008)

    The fine control of caffeine biosynthesis was investigated using caffeine-forming tea callus lines. Feeding of paraxanthine doubled the caffeine level relative to controls. In contrast, no stimulation of caffeine accumulation was observed when the calli were cultured with adenosine, guanosi...
  17. Transgenic tobacco plants producing caffeine: a potential new strategy for insect pest control.

    Transgenic Research 15(6):667 (2006) PMID 17091387

    Caffeine (1,3,7-trimethylxanthine) is one of the most widely used plant secondary metabolites, primarily as a stimulant and an ingredient in drugs. In nature, caffeine is believed to function in chemical defense, acting as an antiherbivory and allelopathic agent, and therefore it might be employ...
  18. Interaction between methyl CpG-binding protein and ran GTPase during cell division in tobacco cultured cells.

    Annals of Botany 98(6):1179 (2006) PMID 17008347 PMCID PMC3292272

    Methyl CpG-binding proteins are considered to play critical roles in epigenetic control of gene expression by recognizing and interacting with 5-methylcytosine (m(5)C) in eukaryotes. However, among 13 corresponding genes in Arabidopsis thaliana, designated as featuring a methyl-binding domain (M...
  19. Caffeine biosynthesis and adenine metabolism in transgenic Coffea canephora plants with reduced expression of N-methyltransferase genes.

    Phytochemistry 67(9):882 (2006) PMID 16624354

    In anti-sense and RNA interference transgenic plants of Coffea canephora in which the expression of CaMXMT1 was suppressed, caffeine biosynthesis from [8-(14)C]adenine was investigated, together with the overall metabolism of [8-(14)C]adenine. Compared with wild type control plants, total purine...
  20. Caffeine biosynthesis and adenine metabolism in transgenicCoffea canephoraplants with reduced expression ofN-methyltransferase genes

    Phytochemistry 67(9):882 (2006)

    Comparison of metabolism of [ 14C]adenine and content of purine compounds in wild type and in antisense and RNAi Coffea canephora transgenic plants is described.