1. An in situ hybridization study of Hyaluronan synthase (Has) mRNA in developing mouse molar and incisor tooth germs.

    Gene Expression Patterns 21(1):28 (2016) PMID 27289075

    Hyaluronan (HA) is a major constituent molecule in most extracellular matrices and is synthesized by Hyaluronan synthase (Has). In the present study, we examined expression patterns of Has1, -2, -3 mRNA in developing mouse molar and incisor tooth germs from embryonic day (E) 11.5 to postnatal da...
  2. Cricoarytenoid Articulation in Elderly Japanese With Special Reference to Morphology of the Synovial Tissue.

    Annals of Otology, Rhinology and Laryngology 125(3):219 (2016) PMID 26391093

    To clarify composite fibers and cells in the synovial tissues of the cricoarytenoid joint (CA joint). Routine histology and immunohistrochemistry using sagittal or nearly sagittal sections obtained from 18 elderly cadaveric specimens. The CA joint capsule was thin and contained few elastic fiber...
  3. Immunohistochemical localization of tenascin-C in rat periodontal ligament with reference to alveolar bone remodeling.

    Anatomical Science International 91(2):196 (2016) PMID 25957016

    We investigated the immunohistochemical localization of tenascin-C in 8-week-old rat periodontal ligaments. Tenascin-C immunoreactivity was detected in zones along with cementum and alveolar bone, and more intensely on the resorption surface of alveolar bone than on the formation surface. On the...
  4. The facilitatory effects of hyperbaric oxygen treatment on membrane bone wound healing in a rat calvarial defect model.

    Undersea & Hyperbaric Medicine 43(2):135 (2016) PMID 27265990

    We examined the effect of hyperbaric oxygen (HBO2) treatment on bone wound healing in a rat calvarial defect. Critical-sized defects were created in the calvaria of adult Wistar rats. The animals were divided into four groups--HBO2, normobaric oxygen, hyperbaric air, and no treatment. Treatments...
  5. Neural-Dural Transition at the Thoracic and Lumbar Spinal Nerve Roots: A Histological Study of Human Late-Stage Fetuses.

    BioMed Research International 2016:8163519 (2016) PMID 27069926 PMCID PMC4812201

    Epidural blocks have been used extensively in infants. However, little histological information is available on the immature neural-dural transition. The neural-dural transition was histologically investigated in 12 late-stage (28-30 weeks) fetuses. The dural sheath of the spinal cord was observ...
  6. CXCR4/CXCL12 signaling impacts enamel progenitor cell proliferation and motility in the dental stem cell niche.

    Cell and Tissue Research 362(3):633 (2015) PMID 26246398

    Dental stem cells are located at the proximal ends of rodent incisors. These stem cells reside in the dental epithelial stem cell niche, termed the apical bud. We focused on identifying critical features of a chemotactic signal in the niche. Here, we report that CXCR4/CXCL12 signaling impacts en...
  7. An Immunohistochemical Study of Matrix Components in Early-Stage Vascular Canals Within Mandibular Condylar Cartilage in Midterm Human Fetuses.

    Anatomical Record. Part A: Advances in Integrat... 298(9):1560 (2015) PMID 25989382

    Matrix components of vascular canals (VCs) in human fetal mandibular condylar cartilage (15-16 weeks of gestation) were analyzed by immunohistochemistry. Prevascular canals (PVCs), consisting of spindle-shaped cells without capillary invasion, were observed within the cartilage. Intense immunore...
  8. Establishment of a novel dwarf rat strain: cartilage calcification insufficient (CCI) rats.

    Experimental Animals 64(2):121 (2015) PMID 25736479 PMCID PMC4427726

    Rats with dwarfism accompanied by skeletal abnormalities, such as shortness of the limbs, tail, and body (dwarf rats), emerged in a Jcl-derived Sprague-Dawley rat colony maintained at the Institute for Animal Experimentation, St. Marianna University Graduate School of Medicine. Since the dwarfis...
  9. Mechanical loading leads to osteoarthritis-like changes in the hypofunctional temporomandibular joint in rats.

    Archives of Oral Biology 59(12):1368 (2014) PMID 25201702

    Temporomandibular joint (TMJ) hypofunction secondary to feeding a liquid diet in the growing period leads to morphological hypoplasia. However, few studies have evaluated the results of mechanical loading on the hypoplastic TMJ. This study investigated whether TMJ hypofunction in rats causes ost...
  10. Distribution of matrix proteins in perichondrium and periosteum during the incorporation of Meckel's cartilage into ossifying mandible in midterm human fetuses: an immunohistochemical study.

    Anatomical Record. Part A: Advances in Integrat... 297(7):1208 (2014) PMID 24700703

    Immunohistochemical localization of versican and tenascin-C were performed; the periosteum of ossifying mandible and the perichondrium of Meckel's cartilage, of vertebral cartilage, and of mandibular condylar cartilage were examined in midterm human fetuses. Versican immunoreactivity was restric...
  11. Expression of carbonic anhydrase IX in human fetal joints, ligaments and tendons: a potential marker of mechanical stress in fetal development?

    Anatomy & Cell Biology 46(4):272 (2013) PMID 24386600 PMCID PMC3875845

    Carbonic anhydrase type IX (CA9) is known to express in the fetal joint cartilage to maintain pH against hypoxia. Using paraffin-embedded histology of 10 human fetuses at 10-16 weeks of gestation with an aid of immunohistochemistry of the intermediate filaments, matrix components (collagen types...
  12. Fetal development of the elastic-fiber-mediated enthesis in the human middle ear.

    Annals of Anatomy - Anatomischer Anzeiger 195(5):441 (2013) PMID 23706648

    In the human middle ear, the annular ligament of the incudostapedial joint and the insertions of the tensor tympani and stapedius muscles contain abundant elastic fibers; i.e., the elastic-fiber-mediated entheses. Hyaluronan also coexists with the elastic fibers. In the present study using immun...
  13. Expression of hyaluronan (hyaluronic acid) in the developing laminar architecture of the human fetal brain.

    Annals of Anatomy - Anatomischer Anzeiger 195(5):424 (2013) PMID 23981810

    Hyaluronan (also called hyaluronic acid or HA) plays a key role in the morphogenesis of the brain, but little is known about its expression in the human fetal neocortex. Using immunohistochemical methods, we assayed the expression of HA, glial fibrillary acidic protein, vimentin, nestin, and pro...
  14. Coexistence of elastic fibers with hyaluronic acid in the human urethral sphincter complex: a histological study.

    The Journal of Urology 190(4):1313 (2013) PMID 23583858

    To promote the prevention and treatment of urethral sphincteric dysfunction, we examined the distribution of elastic fibers around the urethral sphincter complex and the histological localization of hyaluronic acid in relation to elastic fiber architecture. Using elastica-Masson staining as well...
  15. Dense distribution of macrophages in flexor aspects of the hand and foot of mid-term human fetuses.

    Anatomy & Cell Biology 45(4):259 (2012) PMID 23301193 PMCID PMC3531589

    In the developing human musculoskeletal system, cell death with macrophage accumulation occurs in the thigh muscle and interdigital area. To comprehensively clarify the distribution of macrophages, we immunohistochemically examined 16 pairs of upper and lower extremities without the hip joint (l...
  16. Elastic fiber-mediated enthesis in the human middle ear.

    Journal of Anatomy 221(4):331 (2012) PMID 22803514 PMCID PMC3458252

    Adaptation to constant vibration (acoustic oscillation) is likely to confer a specific morphology at the bone-tendon and bone-ligament interfaces at the ear ossicles, which therefore represent an exciting target of enthesis research. We histologically examined (i) the bone attachments of the ten...
  17. Transient appearance of tyrosine hydroxylase immunoreactive cells in the midline epithelial seam of the human fetal secondary palate.

    Cleft Palate-Craniofacial Journal 49(4):414 (2012) PMID 21740166

    Transient immunoreactivity for tyrosine hydroxylase, which mediates the conversion of the amino acid L-tyrosine to dihydroxyphenylalanine, in the midline epithelial seam between the bilateral palatal shelves was investigated in human fetuses. Horizontal or frontal paraffin sections of two human ...
  18. Fetal head anomaly restricted to the eye, the mandible, and the pterygoid process of the sphenoid: a histological study.

    Clinical Anatomy 24(5):599 (2011) PMID 21400609

    A report on an unusual combination of anomalies in the head of a female fetus. The authors examined whole body semiserial paraffin sections of a female fetus (155 mm CRL; ∼18 weeks of gestation), with a particular focus on the head region. Cranial autonomic ganglia, nasal olfactory cells, and th...
  19. Functional analysis of CTRP3/cartducin in Meckel's cartilage and developing condylar cartilage in the fetal mouse mandible.

    Journal of Anatomy 218(5):517 (2011) PMID 21371032 PMCID PMC3089748

    CTRP3/cartducin, a novel C1q family protein, is expressed in proliferating chondrocytes in the growth plate and has an important role in regulating the growth of both chondrogenic precursors and chondrocytes in vitro. We examined the expression of CTRP3/cartducin mRNA in Meckel's cartilage and i...
  20. False positive reactivity of a substance P-antibody in the ectodermal/epithelial plug of the nose, ear, eye and perineum of the human and mouse fetuses.

    Okajimas Folia Anatomica Japonica 87(2):33 (2010) PMID 20882765

    Epithelial/ectodermal plug formation in the developing nose, ear, and eye regions is followed by canalization/recanalization mediated by cell death. However, the mechanism is not well understood. Recently, substance P (SP)-mediated cell death, rather than cell apoptosis, has been reported in neu...