1. FANCJ suppresses microsatellite instability and lymphomagenesis independent of the Fanconi anemia pathway.

    Genes & Development 29(24):2532 (2015) PMID 26637282 PMCID PMC4699383

    Microsatellites are short tandem repeat sequences that are highly prone to expansion/contraction due to their propensity to form non-B-form DNA structures, which hinder DNA polymerases and provoke template slippage. Although error correction by mismatch repair plays a key role in preventing micr...
  2. Molecular basis of telomere dysfunction in human genetic diseases.

    Nature Structural & Molecular Biology 22(11):867 (2015) PMID 26581521

    Mutations in genes encoding proteins required for telomere structure, replication, repair and length maintenance are associated with several debilitating human genetic disorders. These complex telomere biology disorders (TBDs) give rise to critically short telomeres that affect the homeostasis o...
  3. REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans.

    Genes & Development 29(18):1969 (2015) PMID 26385965 PMCID PMC4579353

    The Caenorhabditis elegans gene rec-1 was the first genetic locus identified in metazoa to affect the distribution of meiotic crossovers along the chromosome. We report that rec-1 encodes a distant paralog of HIM-5, which was discovered by whole-genome sequencing and confirmed by multiple genome...
  4. BOD1L Is Required to Suppress Deleterious Resection of Stressed Replication Forks.

    Molecular Cell 59(3):462 (2015) PMID 26166705

    Recognition and repair of damaged replication forks are essential to maintain genome stability and are coordinated by the combined action of the Fanconi anemia and homologous recombination pathways. These pathways are vital to protect stalled replication forks from uncontrolled nucleolytic activ...
  5. Rad51 Paralogs Remodel Pre-synaptic Rad51 Filaments to Stimulate Homologous Recombination.

    Cell 162(2):271 (2015) PMID 26186187 PMCID PMC4518479

    Repair of DNA double strand breaks by homologous recombination (HR) is initiated by Rad51 filament nucleation on single-stranded DNA (ssDNA), which catalyzes strand exchange with homologous duplex DNA. BRCA2 and the Rad51 paralogs are tumor suppressors and critical mediators of Rad51. To gain in...
  6. REV7 counteracts DNA double-strand break resection and affects PARP inhibition.

    Nature 521(7553):541 (2015) PMID 25799992 PMCID PMC4671316

    Error-free repair of DNA double-strand breaks (DSBs) is achieved by homologous recombination (HR), and BRCA1 is an important factor for this repair pathway. In the absence of BRCA1-mediated HR, the administration of PARP inhibitors induces synthetic lethality of tumour cells of patients with bre...
  7. TRF2 recruits RTEL1 to telomeres in S phase to promote t-loop unwinding.

    Molecular Cell 57(4):622 (2015) PMID 25620558 PMCID PMC4339303

    The helicase RTEL1 promotes t-loop unwinding and suppresses telomere fragility to maintain the integrity of vertebrate telomeres. An interaction between RTEL1 and PCNA is important to prevent telomere fragility, but how RTEL1 engages with the telomere to promote t-loop unwinding is unclear. Here...
  8. Homologous-recombination-deficient tumours are dependent on Polθ-mediated repair.

    Nature 518(7538):258 (2015) PMID 25642963 PMCID PMC4415602

    Large-scale genomic studies have shown that half of epithelial ovarian cancers (EOCs) have alterations in genes regulating homologous recombination (HR) repair. Loss of HR accounts for the genomic instability of EOCs and for their cellular hyper-dependence on alternative poly-ADP ribose polymera...
  9. RTEL1: functions of a disease-associated helicase.

    Trends in Cell Biology 24(7):416 (2014) PMID 24582487

    DNA secondary structures that arise during DNA replication, repair, and recombination (3R) must be processed correctly to prevent genetic instability. Regulator of telomere length 1 (RTEL1) is an essential DNA helicase that disassembles a variety of DNA secondary structures to facilitate 3R proc...
  10. Metabolism of DNA secondary structures at the eukaryotic replication fork.

    DNA Repair 19:152 (2014) PMID 24815912

    DNA secondary structures are largely advantageous for numerous cellular processes but can pose specific threats to the progression of the replication machinery and therefore genome duplication and cell division. A number of specialized enzymes dismantle these structures to allow replication fork...
  11. Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.

    Journal of Cell Science 127(Pt 13):2910 (2014) PMID 24816561

    Here, we identify coiled-coil domain-containing protein 13 (Ccdc13) in a genome-wide RNA interference screen for regulators of genome stability. We establish that Ccdc13 is a newly identified centriolar satellite protein that interacts with PCM1, Cep290 and pericentrin and prevents the accumulat...
  12. RTEL1 inhibits trinucleotide repeat expansions and fragility.

    Cell Reports 6(5):827 (2014) PMID 24561255

    Human RTEL1 is an essential, multifunctional helicase that maintains telomeres, regulates homologous recombination, and helps prevent bone marrow failure. Here, we show that RTEL1 also blocks trinucleotide repeat expansions, the causal mutation for 17 neurological diseases. Increased expansion f...
  13. The leukemia-associated Rho guanine nucleotide exchange factor LARG is required for efficient replication stress signaling.

    Cell Cycle 13(21):3450 (2014) PMID 25485589 PMCID PMC4615130

    We previously identified and characterized TELO2 as a human protein that facilitates efficient DNA damage response (DDR) signaling. A subsequent yeast 2-hybrid screen identified LARG; Leukemia-Associated Rho Guanine Nucleotide Exchange Factor (also known as Arhgef12), as a potential novel TELO2 ...
  14. Double-strand break repair: 53BP1 comes into focus.

    Nature Reviews: Molecular Cell Biology 15(1):7 (2014) PMID 24326623

    DNA double-strand break (DSB) signalling and repair is crucial to preserve genomic integrity and maintain cellular homeostasis. p53-binding protein 1 (53BP1) is an important regulator of the cellular response to DSBs that promotes the end-joining of distal DNA ends, which is induced during V(D)J...
  15. Tripartite Motif-containing 33 (TRIM33) protein functions in the poly(ADP-ribose) polymerase (PARP)-dependent DNA damage response through interaction with Amplified in Liver Cancer 1 (ALC1) protein.

    Journal of Biological Chemistry 288(45):32357 (2013) PMID 23926104 PMCID PMC3820871

    Activation of poly(ADP-ribose) polymerase (PARP) near sites of DNA breaks facilitates recruitment of DNA repair proteins and promotes chromatin relaxation in part through the action of chromatin-remodeling enzyme Amplified in Liver Cancer 1 (ALC1). Through proteomic analysis we find that ALC1 in...
  16. HELQ promotes RAD51 paralogue-dependent repair to avert germ cell loss and tumorigenesis.

    Nature 502(7471):381 (2013) PMID 24005329 PMCID PMC3836231

    Repair of interstrand crosslinks (ICLs) requires the coordinated action of the intra-S-phase checkpoint and the Fanconi anaemia pathway, which promote ICL incision, translesion synthesis and homologous recombination (reviewed in refs 1, 2). Previous studies have implicated the 3'-5' superfamily ...
  17. RTEL1 is a replisome-associated helicase that promotes telomere and genome-wide replication.

    Science 342(6155):239 (2013) PMID 24115439

    Regulator of telomere length 1 (RTEL1) is an essential DNA helicase that disassembles telomere loops (T loops) and suppresses telomere fragility to maintain the integrity of chromosome ends. We established that RTEL1 also associates with the replisome through binding to proliferating cell nuclea...
  18. NEK8 links the ATR-regulated replication stress response and S phase CDK activity to renal ciliopathies.

    Molecular Cell 51(4):423 (2013) PMID 23973373 PMCID PMC3790667

    Renal ciliopathies are a leading cause of kidney failure, but their exact etiology is poorly understood. NEK8/NPHP9 is a ciliary kinase associated with two renal ciliopathies in humans and mice, nephronophthisis (NPHP) and polycystic kidney disease. Here, we identify NEK8 as a key effector of th...
  19. A recessive founder mutation in regulator of telomere elongation helicase 1, RTEL1, underlies severe immunodeficiency and features of Hoyeraal Hreidarsson syndrome.

    PLoS Genetics 9(8):e1003695 (2013) PMID 24009516 PMCID PMC3757051

    Dyskeratosis congenita (DC) is a heterogeneous inherited bone marrow failure and cancer predisposition syndrome in which germline mutations in telomere biology genes account for approximately one-half of known families. Hoyeraal Hreidarsson syndrome (HH) is a clinically severe variant of DC in w...
  20. RIF1 is essential for 53BP1-dependent nonhomologous end joining and suppression of DNA double-strand break resection.

    Molecular Cell 49(5):858 (2013) PMID 23333305 PMCID PMC3594748

    The appropriate execution of DNA double-strand break (DSB) repair is critical for genome stability and tumor avoidance. 53BP1 and BRCA1 directly influence DSB repair pathway choice by regulating 5' end resection, but how this is achieved remains uncertain. Here we report that Rif1(-/-) mice are ...