1. Rad51 Paralogs Remodel Pre-synaptic Rad51 Filaments to Stimulate Homologous Recombination.

    Cell 162(2):271 (2015) PMID 26186187

    Repair of DNA double strand breaks by homologous recombination (HR) is initiated by Rad51 filament nucleation on single-stranded DNA (ssDNA), which catalyzes strand exchange with homologous duplex DNA. BRCA2 and the Rad51 paralogs are tumor suppressors and critical mediators of Rad51. To gain in...
  2. REV7 counteracts DNA double-strand break resection and affects PARP inhibition.

    Nature 521(7553):541 (2015) PMID 25799992

    Error-free repair of DNA double-strand breaks (DSBs) is achieved by homologous recombination (HR), and BRCA1 is an important factor for this repair pathway. In the absence of BRCA1-mediated HR, the administration of PARP inhibitors induces synthetic lethality of tumour cells of patients with bre...
  3. TRF2 Recruits RTEL1 to Telomeres in S Phase to Promote T-Loop Unwinding.

    Molecular Cell 57(4):622 (2015) PMID 25620558 PMCID PMC4339303

    The helicase RTEL1 promotes t-loop unwinding and suppresses telomere fragility to maintain the integrity of vertebrate telomeres. An interaction between RTEL1 and PCNA is important to prevent telomere fragility, but how RTEL1 engages with the telomere to promote t-loop unwinding is unclear. Here...
  4. Homologous-recombination-deficient tumours are dependent on Polθ-mediated repair.

    Nature 518(7538):258 (2015) PMID 25642963

    Large-scale genomic studies have shown that half of epithelial ovarian cancers (EOCs) have alterations in genes regulating homologous recombination (HR) repair. Loss of HR accounts for the genomic instability of EOCs and for their cellular hyper-dependence on alternative poly-ADP ribose polymera...
  5. Homologous-recombination-deficient tumours are dependent on Polθ-mediated repair.

    Nature 518(7538):258 (2015) PMID 25642963

    Large-scale genomic studies have shown that half of epithelial ovarian cancers (EOCs) have alterations in genes regulating homologous recombination (HR) repair. Loss of HR accounts for the genomic instability of EOCs and for their cellular hyper-dependence on alternative poly-ADP ribose polymera...
  6. Homologous-recombination-deficient tumours are dependent on Polθ-mediated repair.

    Nature 518(7538):258 (2015) PMID 25642963

    Large-scale genomic studies have shown that half of epithelial ovarian cancers (EOCs) have alterations in genes regulating homologous recombination (HR) repair. Loss of HR accounts for the genomic instability of EOCs and for their cellular hyper-dependence on alternative poly-ADP ribose polymera...
  7. Homologous-recombination-deficient tumours are dependent on Polθ-mediated repair.

    Nature 518(7538):258 (2015) PMID 25642963

    Large-scale genomic studies have shown that half of epithelial ovarian cancers (EOCs) have alterations in genes regulating homologous recombination (HR) repair. Loss of HR accounts for the genomic instability of EOCs and for their cellular hyper-dependence on alternative poly-ADP ribose polymera...
  8. Homologous-recombination-deficient tumours are dependent on Polθ-mediated repair.

    Nature 518(7538):258 (2015) PMID 25642963

    Large-scale genomic studies have shown that half of epithelial ovarian cancers (EOCs) have alterations in genes regulating homologous recombination (HR) repair. Loss of HR accounts for the genomic instability of EOCs and for their cellular hyper-dependence on alternative poly-ADP ribose polymera...
  9. The leukemia-associated Rho guanine nucleotide exchange factor LARG is required for efficient replication stress signaling.

    Cell Cycle 13(21):3450 (2014) PMID 25485589

    We previously identified and characterized TELO2 as a human protein that facilitates efficient DNA damage response (DDR) signaling. A subsequent yeast 2-hybrid screen identified LARG; Leukemia-Associated Rho Guanine Nucleotide Exchange Factor (also known as Arhgef12), as a potential novel TELO2 ...
  10. Metabolism of DNA secondary structures at the eukaryotic replication fork.

    DNA Repair 19:152 (2014) PMID 24815912

    DNA secondary structures are largely advantageous for numerous cellular processes but can pose specific threats to the progression of the replication machinery and therefore genome duplication and cell division. A number of specialized enzymes dismantle these structures to allow replication fork...
  11. Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.

    Journal of Cell Science 127(Pt 13):2910 (2014) PMID 24816561

    Here, we identify coiled-coil domain-containing protein 13 (Ccdc13) in a genome-wide RNA interference screen for regulators of genome stability. We establish that Ccdc13 is a newly identified centriolar satellite protein that interacts with PCM1, Cep290 and pericentrin and prevents the accumulat...
  12. RTEL1: functions of a disease-associated helicase.

    Trends in Cell Biology 24(7):416 (2014) PMID 24582487

    DNA secondary structures that arise during DNA replication, repair, and recombination (3R) must be processed correctly to prevent genetic instability. Regulator of telomere length 1 (RTEL1) is an essential DNA helicase that disassembles a variety of DNA secondary structures to facilitate 3R proc...
  13. Metabolism of DNA secondary structures at the eukaryotic replication fork.

    DNA Repair 19:152 (2014) PMID 24815912

    DNA secondary structures are largely advantageous for numerous cellular processes but can pose specific threats to the progression of the replication machinery and therefore genome duplication and cell division. A number of specialized enzymes dismantle these structures to allow replication fork...
  14. Metabolism of DNA secondary structures at the eukaryotic replication fork.

    DNA Repair 19:152 (2014) PMID 24815912

    DNA secondary structures are largely advantageous for numerous cellular processes but can pose specific threats to the progression of the replication machinery and therefore genome duplication and cell division. A number of specialized enzymes dismantle these structures to allow replication fork...
  15. Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.

    Journal of Cell Science 127(Pt 13):2910 (2014) PMID 24816561

    Here, we identify coiled-coil domain-containing protein 13 (Ccdc13) in a genome-wide RNA interference screen for regulators of genome stability. We establish that Ccdc13 is a newly identified centriolar satellite protein that interacts with PCM1, Cep290 and pericentrin and prevents the accumulat...
  16. RTEL1: functions of a disease-associated helicase.

    Trends in Cell Biology 24(7):416 (2014) PMID 24582487

    DNA secondary structures that arise during DNA replication, repair, and recombination (3R) must be processed correctly to prevent genetic instability. Regulator of telomere length 1 (RTEL1) is an essential DNA helicase that disassembles a variety of DNA secondary structures to facilitate 3R proc...
  17. RTEL1: functions of a disease-associated helicase.

    Trends in Cell Biology 24(7):416 (2014) PMID 24582487

    DNA secondary structures that arise during DNA replication, repair, and recombination (3R) must be processed correctly to prevent genetic instability. Regulator of telomere length 1 (RTEL1) is an essential DNA helicase that disassembles a variety of DNA secondary structures to facilitate 3R proc...
  18. Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.

    Journal of Cell Science 127(Pt 13):2910 (2014) PMID 24816561

    Here, we identify coiled-coil domain-containing protein 13 (Ccdc13) in a genome-wide RNA interference screen for regulators of genome stability. We establish that Ccdc13 is a newly identified centriolar satellite protein that interacts with PCM1, Cep290 and pericentrin and prevents the accumulat...
  19. Phosphorylation-dependent PIH1D1 interactions define substrate specificity of the R2TP cochaperone complex.

    Cell Reports 7(1):19 (2014) PMID 24656813 PMCID PMC3989777

    The R2TP cochaperone complex plays a critical role in the assembly of multisubunit machines, including small nucleolar ribonucleoproteins (snoRNPs), RNA polymerase II, and the mTORC1 and SMG1 kinase complexes, but the molecular basis of substrate recognition remains unclear. Here, we describe a ...
  20. Phosphorylation-dependent PIH1D1 interactions define substrate specificity of the R2TP cochaperone complex.

    Cell Reports 7(1):19 (2014) PMID 24656813 PMCID PMC3989777

    The R2TP cochaperone complex plays a critical role in the assembly of multisubunit machines, including small nucleolar ribonucleoproteins (snoRNPs), RNA polymerase II, and the mTORC1 and SMG1 kinase complexes, but the molecular basis of substrate recognition remains unclear. Here, we describe a ...