1. Membrane nanoclusters-tails of the unexpected.

    Cell 161(3):433 (2015) PMID 25910202

    The existence, nature, and role of highly ordered membrane domains, often referred to as lipid rafts, have been highly debated by cell biologists for many years. In this issue, Raghupathy et al. describe molecular mechanisms leading to the formation of ordered lipid-protein clusters. Copyright ...
  2. Glycan Remodeling with Processing Inhibitors and Lectin-Resistant Eukaryotic Cells.

    Methods in Molecular Biology 1321:307 (2015) PMID 26082231

    Some of the most important and interesting molecules in metazoan biology are glycoproteins. The importance of the carbohydrate component of these structures is often revealed by the disease phenotypes that manifest when the biosynthesis of particular glycoforms is disrupted. On the other hand, t...
  3. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  4. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  5. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  6. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  7. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  8. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  9. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  10. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  11. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  12. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  13. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  14. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  15. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  16. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  17. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  18. Type-3 BRET, an improved competition-based bioluminescence resonance energy transfer assay.

    Biophysical Journal 106(12):L41 (2014) PMID 24940791 PMCID PMC4070276

    We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membr...
  19. TCR signaling: the barrier within.

    Nature Immunology 15(2):136 (2014) PMID 24448571

  20. TCR signaling: the barrier within.

    Nature Immunology 15(2):136 (2014) PMID 24448571