1. Sae2 promotes DNA damage resistance by removing the Mre11-Rad50-Xrs2 complex from DNA and attenuating Rad53 signaling.

    PNAS 112(15):E1880 (2015) PMID 25831494 PMCID PMC4403154

    The Mre11-Rad50-Xrs2/NBS1 (MRX/N) nuclease/ATPase complex plays structural and catalytic roles in the repair of DNA double-strand breaks (DSBs) and is the DNA damage sensor for Tel1/ATM kinase activation. Saccharomyces cerevisiae Sae2 can function with MRX to initiate 5'-3' end resection and als...
  2. Top3-rmi1 dissolve rad51-mediated d loops by a topoisomerase-based mechanism.

    Molecular Cell 57(4):595 (2015) PMID 25699708

    The displacement loop (D loop) is a DNA strand invasion product formed during homologous recombination. Disruption of nascent D loops prevents recombination, and during synthesis-dependent strand annealing (SDSA), disruption of D loops extended by DNA polymerase ensures a non-crossover outcome. ...
  3. Top3-rmi1 dissolve rad51-mediated d loops by a topoisomerase-based mechanism.

    Molecular Cell 57(4):595 (2015) PMID 25699708 PMCID PMC4338411

    The displacement loop (D loop) is a DNA strand invasion product formed during homologous recombination. Disruption of nascent D loops prevents recombination, and during synthesis-dependent strand annealing (SDSA), disruption of D loops extended by DNA polymerase ensures a non-crossover outcome. ...
  4. Top3-Rmi1 Dissolve Rad51-Mediated D Loops by a Topoisomerase-Based Mechanism

    Molecular Cell 57(4):595 (2015)

    The displacement loop (D loop) is a DNA strand invasion product formed during homologous recombination. Disruption of nascent D loops prevents recombination, and during synthesis-dependent strand annealing (SDSA), disruption of D loops extended by DNA polymerase ensures a non-crossover...
  5. Top3-rmi1 dissolve rad51-mediated d loops by a topoisomerase-based mechanism.

    Molecular Cell 57(4):595 (2015) PMID 25699708

    The displacement loop (D loop) is a DNA strand invasion product formed during homologous recombination. Disruption of nascent D loops prevents recombination, and during synthesis-dependent strand annealing (SDSA), disruption of D loops extended by DNA polymerase ensures a non-crossover outcome. ...
  6. Top3-rmi1 dissolve rad51-mediated d loops by a topoisomerase-based mechanism.

    Molecular Cell 57(4):595 (2015) PMID 25699708 PMCID PMC4338411

    The displacement loop (D loop) is a DNA strand invasion product formed during homologous recombination. Disruption of nascent D loops prevents recombination, and during synthesis-dependent strand annealing (SDSA), disruption of D loops extended by DNA polymerase ensures a non-crossover outcome. ...
  7. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.

    Nature Communications 6:7744 (2015) PMID 26215093

    MCM8-9 complex is required for homologous recombination (HR)-mediated repair of double-strand breaks (DSBs). Here we report that MCM8-9 is required for DNA resection by MRN (MRE11-RAD50-NBS1) at DSBs to generate ssDNA. MCM8-9 interacts with MRN and is required for the nuclease activity and stabl...
  8. RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination.

    PNAS 111(48):E5133 (2014) PMID 25411316

    Recombinational DNA repair by the RecF pathway of Escherichia coli requires the coordinated activities of RecA, RecFOR, RecQ, RecJ, and single-strand DNA binding (SSB) proteins. These proteins facilitate formation of homologously paired joint molecules between linear double-stranded (dsDNA) and ...
  9. RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination.

    PNAS 111(48):E5133 (2014) PMID 25411316 PMCID PMC4260596

    Recombinational DNA repair by the RecF pathway of Escherichia coli requires the coordinated activities of RecA, RecFOR, RecQ, RecJ, and single-strand DNA binding (SSB) proteins. These proteins facilitate formation of homologously paired joint molecules between linear double-stranded (dsDNA) and ...
  10. RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination.

    PNAS 111(48):E5133 (2014) PMID 25411316 PMCID PMC4260596

    Recombinational DNA repair by the RecF pathway of Escherichia coli requires the coordinated activities of RecA, RecFOR, RecQ, RecJ, and single-strand DNA binding (SSB) proteins. These proteins facilitate formation of homologously paired joint molecules between linear double-stranded (dsDNA) and ...
  11. RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination.

    PNAS 111(48):E5133 (2014) PMID 25411316 PMCID PMC4260596

    Recombinational DNA repair by the RecF pathway of Escherichia coli requires the coordinated activities of RecA, RecFOR, RecQ, RecJ, and single-strand DNA binding (SSB) proteins. These proteins facilitate formation of homologously paired joint molecules between linear double-stranded (dsDNA) and ...
  12. Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase IIIα and RMI1.

    Nature Structural & Molecular Biology 21(3):261 (2014) PMID 24509834 PMCID PMC4292918

    Repair of DNA double-strand breaks via homologous recombination can produce double Holliday junctions (dHJs) that require enzymatic separation. Topoisomerase IIIα (TopIIIα) together with RMI1 disentangles the final hemicatenane intermediate obtained once dHJs have converged. How binding of RMI1 ...
  13. Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase IIIα and RMI1.

    Nature Structural & Molecular Biology 21(3):261 (2014) PMID 24509834

    Repair of DNA double-strand breaks via homologous recombination can produce double Holliday junctions (dHJs) that require enzymatic separation. Topoisomerase IIIα (TopIIIα) together with RMI1 disentangles the final hemicatenane intermediate obtained once dHJs have converged. How binding of RMI1 ...
  14. Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase IIIα and RMI1.

    Nature Structural & Molecular Biology 21(3):261 (2014) PMID 24509834 PMCID PMC4292918

    Repair of DNA double-strand breaks via homologous recombination can produce double Holliday junctions (dHJs) that require enzymatic separation. Topoisomerase IIIα (TopIIIα) together with RMI1 disentangles the final hemicatenane intermediate obtained once dHJs have converged. How binding of RMI1 ...
  15. Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase IIIα and RMI1.

    Nature Structural & Molecular Biology 21(3):261 (2014) PMID 24509834 PMCID PMC4292918

    Repair of DNA double-strand breaks via homologous recombination can produce double Holliday junctions (dHJs) that require enzymatic separation. Topoisomerase IIIα (TopIIIα) together with RMI1 disentangles the final hemicatenane intermediate obtained once dHJs have converged. How binding of RMI1 ...
  16. Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase IIIα and RMI1.

    Nature Structural & Molecular Biology 21(3):261 (2014) PMID 24509834

    Repair of DNA double-strand breaks via homologous recombination can produce double Holliday junctions (dHJs) that require enzymatic separation. Topoisomerase IIIα (TopIIIα) together with RMI1 disentangles the final hemicatenane intermediate obtained once dHJs have converged. How binding of RMI1 ...
  17. Nucleosome Positioning on Lambda DNA for Single-Molecule Analysis of Chromatin Remodeling - Development of a Versatile Lambda DNA Construct Capable of Reception of any DNA Sequences of Interest

    Biophysical Journal 106(2):70a (2014)

  18. DNA unwinding heterogeneity by RecBCD results from static molecules able to equilibrate.

    Nature 500(7463):482 (2013) PMID 23851395 PMCID PMC3779544

    Single-molecule studies can overcome the complications of asynchrony and ensemble-averaging in bulk-phase measurements, provide mechanistic insights into molecular activities, and reveal interesting variations between individual molecules. The application of these techniques to the RecBCD helica...
  19. DNA unwinding heterogeneity by RecBCD results from static molecules able to equilibrate.

    Nature 500(7463):482 (2013) PMID 23851395 PMCID PMC3779544

    Single-molecule studies can overcome the complications of asynchrony and ensemble-averaging in bulk-phase measurements, provide mechanistic insights into molecular activities, and reveal interesting variations between individual molecules. The application of these techniques to the RecBCD helica...
  20. Pif1 family helicases suppress genome instability at G-quadruplex motifs.

    Nature 497(7450):458 (2013) PMID 23657261 PMCID PMC3680789

    The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by...