1. Rph1/KDM4 Mediates Nutrient-Limitation Signaling that Leads to the Transcriptional Induction of Autophagy.

    Current Biology 25(5):546 (2015) PMID 25660547 PMCID PMC4348152

    Autophagy is a conserved process mediating vacuolar degradation and recycling. Autophagy is highly upregulated upon various stresses and is essential for cell survival in deleterious conditions. Autophagy defects are associated with severe pathologies, whereas unchecked autophagy activity causes...
  2. Atg23 and Atg27 Act at the Early Stages of Atg9 Trafficking in S. cerevisiae.

    Traffic 16(2):172 (2015) PMID 25385507 PMCID PMC4305007

    Atg9 is a conserved multipass transmembrane protein with an essential role in autophagy. In Saccharomyces cerevisiae, it travels through the secretory pathway to a unique compartment, the Atg9 peripheral structures. These structures are then targeted to the phagophore assembly site (PAS), where ...
  3. Atg23 and Atg27 act at the early stages of Atg9 trafficking in S. cerevisiae.

    Traffic 16(2):172 (2015) PMID 25385507 PMCID PMC4305007

    Atg9 is a conserved multipass transmembrane protein with an essential role in autophagy. In Saccharomyces cerevisiae, it travels through the secretory pathway to a unique compartment, the Atg9 peripheral structures. These structures are then targeted to the phagophore assembly site (PAS), where ...
  4. High lipid order of Arabidopsis cell-plate membranes mediated by sterol and DYNAMIN-RELATED PROTEIN1A function.

    Plant Journal 80(5):745 (2014) PMID 25234576 PMCID PMC4280860

    Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant...
  5. High lipid order of Arabidopsis cell-plate membranes mediated by sterol and DYNAMIN-RELATED PROTEIN1A function.

    Plant Journal 80(5):745 (2014) PMID 25234576

    Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant...
  6. High lipid order of Arabidopsis cell-plate membranes mediated by sterol and DYNAMIN-RELATED PROTEIN1A function.

    Plant Journal 80(5):745 (2014) PMID 25234576

    Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant...
  7. Loss of Arabidopsis thaliana Dynamin-Related Protein 2B reveals separation of innate immune signaling pathways.

    PLoS Pathogens 10(12):e1004578 (2014) PMID 25521759 PMCID PMC4270792

    Vesicular trafficking has emerged as an important means by which eukaryotes modulate responses to microbial pathogens, likely by contributing to the correct localization and levels of host components necessary for effective immunity. However, considering the complexity of membrane trafficking in...
  8. Loss of Arabidopsis thaliana Dynamin-Related Protein 2B Reveals Separation of Innate Immune Signaling Pathways.

    PLoS Pathogens 10(12):e1004578 (2014) PMID 25521759 PMCID PMC4270792

    Vesicular trafficking has emerged as an important means by which eukaryotes modulate responses to microbial pathogens, likely by contributing to the correct localization and levels of host components necessary for effective immunity. However, considering the complexity of membrane trafficking in...
  9. Loss of Arabidopsis thaliana Dynamin-Related Protein 2B Reveals Separation of Innate Immune Signaling Pathways.

    PLoS Pathogens 10(12):e1004578 (2014) PMID 25521759 PMCID PMC4270792

    Vesicular trafficking has emerged as an important means by which eukaryotes modulate responses to microbial pathogens, likely by contributing to the correct localization and levels of host components necessary for effective immunity. However, considering the complexity of membrane trafficking in...
  10. High lipid order of Arabidopsis cell-plate membranes mediated by sterol and DYNAMIN-RELATED PROTEIN1A function.

    Plant Journal 80(5):745 (2014) PMID 25234576 PMCID PMC4280860

    Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant...
  11. Loss of Arabidopsis thaliana Dynamin-Related Protein 2B reveals separation of innate immune signaling pathways.

    PLoS Pathogens 10(12):e1004578 (2014) PMID 25521759 PMCID PMC4270792

    Vesicular trafficking has emerged as an important means by which eukaryotes modulate responses to microbial pathogens, likely by contributing to the correct localization and levels of host components necessary for effective immunity. However, considering the complexity of membrane trafficking in...
  12. Loss of Arabidopsis thaliana Dynamin-Related Protein 2B Reveals Separation of Innate Immune Signaling Pathways.

    PLoS Pathogens 10(12):e1004578 (2014) PMID 25521759

    Vesicular trafficking has emerged as an important means by which eukaryotes modulate responses to microbial pathogens, likely by contributing to the correct localization and levels of host components necessary for effective immunity. However, considering the complexity of membrane trafficking in...
  13. Transcriptional regulation by Pho23 modulates the frequency of autophagosome formation.

    Current Biology 24(12):1314 (2014) PMID 24881874 PMCID PMC4169046

    Autophagy as a conserved lysosomal/vacuolar degradation and recycling pathway is important in normal development and physiology, and defects in this process are linked to many kinds of disease. Because too much or too little autophagy can be detrimental, the process must be tightly regulated bot...
  14. Transcriptional regulation by Pho23 modulates the frequency of autophagosome formation.

    Current Biology 24(12):1314 (2014) PMID 24881874 PMCID PMC4169046

    Autophagy as a conserved lysosomal/vacuolar degradation and recycling pathway is important in normal development and physiology, and defects in this process are linked to many kinds of disease. Because too much or too little autophagy can be detrimental, the process must be tightly regulated bot...
  15. Estimating the size and number of autophagic bodies by electron microscopy.

    Autophagy 10(1):155 (2014) PMID 24270884

    Much recent and ongoing research is focused on understanding the mechanisms and regulation of autophagy, a cellular self-degradation pathway with many links to human health. Although many assays exist to measure the total magnitude of autophagy, electron microscopy remains the tool of choice for...
  16. Estimating the size and number of autophagic bodies by electron microscopy.

    Autophagy 10(1):155 (2014) PMID 24270884

    Much recent and ongoing research is focused on understanding the mechanisms and regulation of autophagy, a cellular self-degradation pathway with many links to human health. Although many assays exist to measure the total magnitude of autophagy, electron microscopy remains the tool of choice for...
  17. The Ume6-Sin3-Rpd3 complex regulates ATG8 transcription to control autophagosome size.

    Autophagy 8(12):1835 (2012) PMID 22960621 PMCID PMC3541296

    The vast majority of studies addressing the induction of autophagy have focused upon cytoplasmic aspects of its regulation. Recently, we have started to expand our knowledge regarding the nuclear events of autophagic induction. Many autophagy-related genes are transcriptionally upregulated upon ...
  18. The Ume6-Sin3-Rpd3 complex regulates ATG8 transcription to control autophagosome size.

    Autophagy 8(12):1835 (2012) PMID 22960621 PMCID PMC3541296

    The vast majority of studies addressing the induction of autophagy have focused upon cytoplasmic aspects of its regulation. Recently, we have started to expand our knowledge regarding the nuclear events of autophagic induction. Many autophagy-related genes are transcriptionally upregulated upon ...
  19. Atg11: a Rab-dependent, coiled-coil membrane protein that acts as a tether for autophagy.

    Autophagy 8(8):1275 (2012) PMID 22717525 PMCID PMC3679242

    Selective macroautophagy uses double-membrane vesicles, termed autophagosomes, to transport cytoplasmic pathogens, organelles and protein complexes to the vacuole for degradation. Autophagosomes are formed de novo by membrane fusion events at the phagophore assembly site (PAS). Therefore, precur...
  20. Atg11: a Rab-dependent, coiled-coil membrane protein that acts as a tether for autophagy.

    Autophagy 8(8):1275 (2012) PMID 22717525 PMCID PMC3679242

    Selective macroautophagy uses double-membrane vesicles, termed autophagosomes, to transport cytoplasmic pathogens, organelles and protein complexes to the vacuole for degradation. Autophagosomes are formed de novo by membrane fusion events at the phagophore assembly site (PAS). Therefore, precur...