1. Neutrophils exacerbate tuberculosis infection in genetically susceptible mice.

    Tuberculosis 95(4):447 (2015) PMID 25935122

    Mice of the I/St inbred strain genetically hyper-susceptible to TB infection and prone to form neutrophil-abundant necrotic lung lesions and relatively resistant mice of the C57BL/6 (B6) strain were infected with 100 CFU of M. tuberculosis H37Rv. To verify the role of neutrophils in TB immunity,...
  2. Reactivation of dormant "non-culturable" Mycobacterium tuberculosis developed in vitro after injection in mice: both the dormancy depth and host genetics influence the outcome.

    Microbial Pathogenesis 78:63 (2015) PMID 25434928

    Three stocks of Mycobacterium tuberculosis H37Rv were cultured in vitro under prolonged hypoxic or acidified conditions until partial or complete loss of the capacity to form colonies on agar medium was achieved. Such dormant "non-culturable" mycobacteria were assessed for the growth resuscitati...
  3. Latent tuberculosis infection: what we know about its genetic control?

    Tuberculosis 94(5):462 (2014) PMID 25104213

    About 90% of all cases of tuberculosis (TB) infection are comprised of latent mycobacterial persistence in the absence of clinical manifestations. In a proportion of latently infected individuals infection eventually reactivates and becomes contagious, seriously influencing epidemiological situa...
  4. Mycobacterium tuberculosisattenuated by multiple deletions ofrpfgenes effectively protects mice against TB infection

    Tuberculosis 91(3):219 (2011) PMID 21419703

    In this study, we investigated the residual virulence of mutants of Mycobacterium tuberculosis that are defective in 4 of the 5 rpf-like genes, their capacity to persist in the murine host and the utility present in these mutants to serve as novel vaccine candidates. Our data ...
  5. Mycobacterium tuberculosisattenuated by multiple deletions ofrpfgenes effectively protects mice against TB infection

    Tuberculosis 91(3):219 (2011) PMID 21419703

    In this study, we investigated the residual virulence of mutants of Mycobacterium tuberculosis that are defective in 4 of the 5 rpf-like genes, their capacity to persist in the murine host and the utility present in these mutants to serve as novel vaccine candidates. Our data ...
  6. Specificity and efficacy of dendritic cell-based vaccination against tuberculosis with complex mycobacterial antigens in a mouse model.

    Tuberculosis 87(2):134 (2007) PMID 17011827

    Dendritic cells (DC) likely play important and unique roles in the generation of protective immunity to mycobacteria. In order to clarify their contributions, bone marrow-derived DC loaded with Mycobacterium tuberculosis sonicate antigens were used to stimulate T cell proliferation both in vitro...
  7. Specificity and efficacy of dendritic cell-based vaccination against tuberculosis with complex mycobacterial antigens in a mouse model

    Tuberculosis 87(2):134 (2007)

    Dendritic cells (DC) likely play important and unique roles in the generation of protective immunity to mycobacteria. In order to clarify their contributions, bone marrow-derived DC loaded with Mycobacterium tuberculosis sonicate antigens were used to stimulate T cell proliferation b...