RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability.
Chromosome Research 24(2):161 (2016)
RbAp46/48, histone chaperone, is a family of evolutionarily conserved WD40 repeat-containing proteins, which are involved in various chromatin-metabolizing processes, but their in vivo functional relevance is yet unclear. In order to examine the biological role of pRbAp48 in chicken DT40 cells, ...
Genetic complementation analysis showed distinct contributions of the N-terminal tail of H2A.Z to epigenetic regulations.
Genes to Cells 21(2):122 (2016)
H2A.Z is one of the most evolutionally conserved histone variants. In vertebrates, this histone variant has two isoforms, H2A.Z.1 and H2A.Z.2, each of which is coded by an individual gene. H2A.Z is involved in multiple epigenetic regulations, and in humans, it also has relevance to carcinogenesi...
Chromatin binding of RCC1 during mitosis is important for its nuclear localization in interphase.
Molecular Biology of the Cell 27(2):371 (2016)
RCC1, a guanine nucleotide exchange factor of the small GTPase Ran, plays various roles throughout the cell cycle. However, the functions of RCC1 in biological processes in vivo are still unclear. In particular, although RCC1 has multifunctional domains, the biological significance of each domai...
Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes.
Methods in Molecular Biology 1413:135 (2016)
The kinetochore connects chromosomes to microtubules during mitosis and therefore plays an essential role in faithful chromosome segregation. It is built at the centromeric region of the chromosome and is comprised of many protein complexes. CENP-S, -T, -W, and -X are kinetochore components with...
Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability.
Open Biology 6(1):150230 (2016)
Most studies using knockout technologies to examine protein function have relied either on shutting off transcription (conventional conditional knockouts with tetracycline-regulated gene expression or gene disruption) or destroying the mature mRNA (RNAi technology). In both cases, the target pro...
Chicken rRNA Gene Cluster Structure.
PLoS ONE 11(6):e0157464 (2016)
Ribosomal RNA (rRNA) genes, whose activity results in nucleolus formation, constitute an extremely important part of genome. Despite the extensive exploration into avian genomes, no complete description of avian rRNA gene primary structure has been offered so far. We publish a complete chicken r...
Whole-proteome genetic analysis of dependencies in assembly of a vertebrate kinetochore.
Journal of Cell Biology 211(6):1141 (2015)
Kinetochores orchestrate mitotic chromosome segregation. Here, we use quantitative mass spectrometry of mitotic chromosomes isolated from a comprehensive set of chicken DT40 mutants to examine the dependencies of 93 confirmed and putative kinetochore proteins for stable association with chromoso...
A new Xist allele driven by a constitutively active promoter is dominated by Xist locus environment and exhibits the parent-of-origin effects.
Development 142(24):4299 (2015)
The dosage difference of X-linked genes between the sexes in mammals is compensated for by genetic inactivation of one of the X chromosomes in XX females. A noncoding RNA transcribed from the Xist gene at the onset of X chromosome inactivation coats the X chromosome in cis and induces chromosome...
Current Biology 25(21):R1021 (2015)
Current Biology 25(21):R1021 (2015)
Tatsuo Fukagawa studies centromeres and kinetochores at the Graduate School of Frontier Bioscience in Osaka University.
Dynamic changes in CCAN organization through CENP-C during cell-cycle progression.
Molecular Biology of the Cell 26(21):3768 (2015)
The kinetochore is a crucial structure for faithful chromosome segregation during mitosis and is formed in the centromeric region of each chromosome. The 16-subunit protein complex known as the constitutive centromere-associated network (CCAN) forms the foundation for kinetochore assembly on the...
HJURP is involved in the expansion of centromeric chromatin.
Molecular Biology of the Cell 26(15):2742 (2015)
The CENP-A-specific chaperone HJURP mediates CENP-A deposition at centromeres. The N-terminal region of HJURP is responsible for binding to soluble CENP-A. However, it is unclear whether other regions of HJURP have additional functions for centromere formation and maintenance. In this study, we ...
Cell Division: A New Role for the Kinetochore in Central Spindle Assembly.
Current Biology 25(13):R554 (2015)
The central spindle, which is formed between segregating chromosomes, is a critical structure for cell division. However, it was unclear how the central spindle is assembled at anaphase onset. A recent study reveals that a conserved kinetochore protein network plays an essential role in initiati...
CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.
Molecular Biology of the Cell 26(7):1225 (2015)
Centrosomes nucleate spindle formation, direct spindle pole positioning, and are important for proper chromosome segregation during mitosis in most animal cells. We previously reported that centromere protein 32 (CENP-32) is required for centrosome association with spindle poles during metaphase...
Current Biology 24(19):R946 (2014)
Neocentromeres are formed at new positions on a chromosome, and offer unique opportunities to study the regulation of centromere formation.
The centromere: chromatin foundation for the kinetochore machinery.
Developmental Cell 30(5):496 (2014)
Since discovery of the centromere-specific histone H3 variant CENP-A, centromeres have come to be defined as chromatin structures that establish the assembly site for the complex kinetochore machinery. In most organisms, centromere activity is defined epigenetically, rather than by specific DNA ...
The CENP-O complex requirement varies among different cell types.
Chromosome Research 22(3):293 (2014)
CENP-U (CENP-50) is a component of the CENP-O complex, which includes CENP-O, CENP-P, CENP-Q, CENP-R, and CENP-U and is constitutively localized at kinetochores throughout the cell cycle in vertebrates. Although CENP-U deficiency results in some mitotic defects in chicken DT40 cells, CENP-U-defi...
Reorganization of damaged chromatin by the exchange of histone variant H2A.Z-2.
International Journal of Radiation Oncology*Bio... 89(4):736 (2014)
The reorganization of damaged chromatin plays an important role in the regulation of the DNA damage response. A recent study revealed the presence of 2 vertebrate H2A.Z isoforms, H2A.Z-1 and H2A.Z-2. However, the roles of the vertebrate H2A.Z isoforms are still unclear. Thus, in this study we ex...
Histone H4 Lys 20 monomethylation of the CENP-A nucleosome is essential for kinetochore assembly.
Developmental Cell 29(6):740 (2014)
In vertebrate cells, centromeres are specified epigenetically through the deposition of the centromere-specific histone CENP-A. Following CENP-A deposition, additional proteins are assembled on centromeric chromatin. However, it remains unknown whether additional epigenetic features of centromer...
The histone-fold complex MHF is remodeled by FANCM to recognize branched DNA and protect genome stability.
Cell Research 24(5):560 (2014)
Histone-fold proteins typically assemble in multiprotein complexes to bind duplex DNA. However, one histone-fold complex, MHF, associates with Fanconi anemia (FA) protein FANCM to form a branched DNA remodeling complex that senses and repairs stalled replication forks and activates FA DNA damage...