1. RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability.

    Chromosome Research 24(2):161 (2016) PMID 26667624

    RbAp46/48, histone chaperone, is a family of evolutionarily conserved WD40 repeat-containing proteins, which are involved in various chromatin-metabolizing processes, but their in vivo functional relevance is yet unclear. In order to examine the biological role of pRbAp48 in chicken DT40 cells, ...
  2. Genetic complementation analysis showed distinct contributions of the N-terminal tail of H2A.Z to epigenetic regulations.

    Genes to Cells 21(2):122 (2016) PMID 26833946

    H2A.Z is one of the most evolutionally conserved histone variants. In vertebrates, this histone variant has two isoforms, H2A.Z.1 and H2A.Z.2, each of which is coded by an individual gene. H2A.Z is involved in multiple epigenetic regulations, and in humans, it also has relevance to carcinogenesi...
  3. Chromatin binding of RCC1 during mitosis is important for its nuclear localization in interphase.

    Molecular Biology of the Cell 27(2):371 (2016) PMID 26564799 PMCID PMC4713138

    RCC1, a guanine nucleotide exchange factor of the small GTPase Ran, plays various roles throughout the cell cycle. However, the functions of RCC1 in biological processes in vivo are still unclear. In particular, although RCC1 has multifunctional domains, the biological significance of each domai...
  4. Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes.

    Methods in Molecular Biology 1413:135 (2016) PMID 27193847

    The kinetochore connects chromosomes to microtubules during mitosis and therefore plays an essential role in faithful chromosome segregation. It is built at the centromeric region of the chromosome and is comprised of many protein complexes. CENP-S, -T, -W, and -X are kinetochore components with...
  5. Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability.

    Open Biology 6(1):150230 (2016) PMID 26791246 PMCID PMC4736828

    Most studies using knockout technologies to examine protein function have relied either on shutting off transcription (conventional conditional knockouts with tetracycline-regulated gene expression or gene disruption) or destroying the mature mRNA (RNAi technology). In both cases, the target pro...
  6. Chicken rRNA Gene Cluster Structure.

    PLoS ONE 11(6):e0157464 (2016) PMID 27299357 PMCID PMC4907446

    Ribosomal RNA (rRNA) genes, whose activity results in nucleolus formation, constitute an extremely important part of genome. Despite the extensive exploration into avian genomes, no complete description of avian rRNA gene primary structure has been offered so far. We publish a complete chicken r...
  7. Whole-proteome genetic analysis of dependencies in assembly of a vertebrate kinetochore.

    Journal of Cell Biology 211(6):1141 (2015) PMID 26668330 PMCID PMC4687880

    Kinetochores orchestrate mitotic chromosome segregation. Here, we use quantitative mass spectrometry of mitotic chromosomes isolated from a comprehensive set of chicken DT40 mutants to examine the dependencies of 93 confirmed and putative kinetochore proteins for stable association with chromoso...
  8. A new Xist allele driven by a constitutively active promoter is dominated by Xist locus environment and exhibits the parent-of-origin effects.

    Development 142(24):4299 (2015) PMID 26511926

    The dosage difference of X-linked genes between the sexes in mammals is compensated for by genetic inactivation of one of the X chromosomes in XX females. A noncoding RNA transcribed from the Xist gene at the onset of X chromosome inactivation coats the X chromosome in cis and induces chromosome...
  9. Tatsuo Fukagawa.

    Current Biology 25(21):R1021 (2015) PMID 26839939

  10. Tatsuo Fukagawa

    Current Biology 25(21):R1021 (2015)

    Tatsuo Fukagawa studies centromeres and kinetochores at the Graduate School of Frontier Bioscience in Osaka University.
  11. Dynamic changes in CCAN organization through CENP-C during cell-cycle progression.

    Molecular Biology of the Cell 26(21):3768 (2015) PMID 26354420 PMCID PMC4626062

    The kinetochore is a crucial structure for faithful chromosome segregation during mitosis and is formed in the centromeric region of each chromosome. The 16-subunit protein complex known as the constitutive centromere-associated network (CCAN) forms the foundation for kinetochore assembly on the...
  12. HJURP is involved in the expansion of centromeric chromatin.

    Molecular Biology of the Cell 26(15):2742 (2015) PMID 26063729 PMCID PMC4571335

    The CENP-A-specific chaperone HJURP mediates CENP-A deposition at centromeres. The N-terminal region of HJURP is responsible for binding to soluble CENP-A. However, it is unclear whether other regions of HJURP have additional functions for centromere formation and maintenance. In this study, we ...
  13. Cell Division: A New Role for the Kinetochore in Central Spindle Assembly.

    Current Biology 25(13):R554 (2015) PMID 26126279

    The central spindle, which is formed between segregating chromosomes, is a critical structure for cell division. However, it was unclear how the central spindle is assembled at anaphase onset. A recent study reveals that a conserved kinetochore protein network plays an essential role in initiati...
  14. CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.

    Molecular Biology of the Cell 26(7):1225 (2015) PMID 25657325 PMCID PMC4454171

    Centrosomes nucleate spindle formation, direct spindle pole positioning, and are important for proper chromosome segregation during mitosis in most animal cells. We previously reported that centromere protein 32 (CENP-32) is required for centrosome association with spindle poles during metaphase...
  15. Neocentromeres

    Current Biology 24(19):R946 (2014) PMID 25291631

    Neocentromeres are formed at new positions on a chromosome, and offer unique opportunities to study the regulation of centromere formation.
  16. The centromere: chromatin foundation for the kinetochore machinery.

    Developmental Cell 30(5):496 (2014) PMID 25203206 PMCID PMC4160344

    Since discovery of the centromere-specific histone H3 variant CENP-A, centromeres have come to be defined as chromatin structures that establish the assembly site for the complex kinetochore machinery. In most organisms, centromere activity is defined epigenetically, rather than by specific DNA ...
  17. The CENP-O complex requirement varies among different cell types.

    Chromosome Research 22(3):293 (2014) PMID 24481920 PMCID PMC4129241

    CENP-U (CENP-50) is a component of the CENP-O complex, which includes CENP-O, CENP-P, CENP-Q, CENP-R, and CENP-U and is constitutively localized at kinetochores throughout the cell cycle in vertebrates. Although CENP-U deficiency results in some mitotic defects in chicken DT40 cells, CENP-U-defi...
  18. Reorganization of damaged chromatin by the exchange of histone variant H2A.Z-2.

    International Journal of Radiation Oncology*Bio... 89(4):736 (2014) PMID 24969791

    The reorganization of damaged chromatin plays an important role in the regulation of the DNA damage response. A recent study revealed the presence of 2 vertebrate H2A.Z isoforms, H2A.Z-1 and H2A.Z-2. However, the roles of the vertebrate H2A.Z isoforms are still unclear. Thus, in this study we ex...
  19. Histone H4 Lys 20 monomethylation of the CENP-A nucleosome is essential for kinetochore assembly.

    Developmental Cell 29(6):740 (2014) PMID 24960696 PMCID PMC4081567

    In vertebrate cells, centromeres are specified epigenetically through the deposition of the centromere-specific histone CENP-A. Following CENP-A deposition, additional proteins are assembled on centromeric chromatin. However, it remains unknown whether additional epigenetic features of centromer...
  20. The histone-fold complex MHF is remodeled by FANCM to recognize branched DNA and protect genome stability.

    Cell Research 24(5):560 (2014) PMID 24699063 PMCID PMC4011343

    Histone-fold proteins typically assemble in multiprotein complexes to bind duplex DNA. However, one histone-fold complex, MHF, associates with Fanconi anemia (FA) protein FANCM to form a branched DNA remodeling complex that senses and repairs stalled replication forks and activates FA DNA damage...