1. Whole-Genome Sequencing Reveals the Origin and Rapid Evolution of an Emerging Outbreak Strain of Streptococcus pneumoniae 12F.

    Clinical Infectious Diseases 62(9):1126 (2016) PMID 26908785

    Streptococcus pneumoniaeis a major cause of community-acquired pneumonia and septicemia in adults. The global drug-susceptible capsular serotype 12F, clonal complex 218 caused several outbreaks in the United States between 1989 and 2008, as well as a recent large outbreak in Manitoba, Canada, th...
  2. New Delhi metallo-β-lactamase in Jamaica.

    The Journal of Infection in Developing Countries 10(2):183 (2016) PMID 26927461

    The global dissemination of the New Delhi metallo-beta-lactamase (NDM) gene among certain strains of bacteria has serious implications since the infections caused by such organisms pose a therapeutic challenge. Although the NDM gene has been detected in various parts of the world, this is the fi...
  3. Implementation of Next-Generation Sequencing for Hepatitis B Virus Resistance Testing and Genotyping in a Clinical Microbiology Laboratory.

    Journal of Clinical Microbiology 54(1):127 (2016) PMID 26537448 PMCID PMC4702765

    Sanger sequencing or DNA hybridization have been the primary modalities for hepatitis B (HBV) resistance testing and genotyping; however, there are limitations, such as low sensitivity and the inability to detect novel mutations. Next-generation sequencing (NGS) for HBV can overcome these limita...
  4. The Effectiveness of Culture-Directed Preemptive Anti-Aspergillus Treatment in Lung Transplant Recipients at One Year After Transplant.

    Transplantation 99(11):2387 (2015) PMID 25961491

    Invasive pulmonary aspergillosis (IPA) is a significant complication after lung transplantation. However, the risk factors for IPA in patients colonized with Aspergillus species, and the effectiveness of culture-directed preemptive treatment, are not well known. We studied 328 lung transplant re...
  5. Detection of enterovirus D68 in Canadian laboratories.

    Journal of Clinical Microbiology 53(5):1748 (2015) PMID 25740765 PMCID PMC4400745

    The recent emergence of a severe respiratory disease caused by enterovirus D68 prompted investigation into whether Canadian hospital and provincial laboratories can detect this virus using commercial and laboratory-developed assays. This study demonstrated analytical sensitivity differences betw...
  6. Time-dependent bias in hepatitis C classification.

    Epidemiology 26(2):e24 (2015) PMID 25643117

  7. Hepatitis C virus seroconversion among HIV-positive men who have sex with men with no history of injection drug use: Results from a clinical HIV cohort.

    The Canadian journal of infectious diseases = J... 26(1):17 (2015) PMID 25798149 PMCID PMC4353264

    Internationally, there is a growing recognition that hepatitis C virus (HCV) may be sexually transmitted among HIV-positive men who have sex with men (MSM). To report the first Canadian estimate of HCV seroincidence in 2000 to 2010 and its risk factors among HIV-positive MSM with no known histor...
  8. High incidence of diagnosis with syphilis co-infection among men who have sex with men in an HIV cohort in Ontario, Canada.

    BMC Infectious Diseases 15:356 (2015) PMID 26289937 PMCID PMC4546079

    The re-emergence of syphilis among HIV-positive gay and other men who have sex with men (MSM) requires vigilance. We estimated incidence of and risk factors for first and subsequent syphilis diagnoses among MSM in HIV care in Ontario, Canada. We analyzed data from 2,280 MSM under follow-up from ...
  9. Utility of a monitoring strategy for human herpesviruses 6 and 7 viremia after liver transplantation: a randomized clinical trial.

    Transplantation 99(1):106 (2015) PMID 25073037

    Reactivation of human herpesvirus (HHV)-6 and HHV-7 has been linked to various posttransplant adverse events through immunomodulatory effects. The potential utility of monitoring for HHV-6 and HHV-7 viremia remains unclear. In this clinical trial, 129 liver transplant recipients were randomized ...
  10. Modest rise in chlamydia and gonorrhoea testing did not increase case detection in a clinical HIV cohort in Ontario, Canada.

    Sexually Transmitted Infections 90(8):608 (2014) PMID 25178285 PMCID PMC4251188

    We described patterns of testing for chlamydia and gonorrhoea infection among persons in specialty HIV care in Ontario, Canada, from 2008 to 2011. We analysed data from 3165 participants in the OHTN Cohort Study attending one of seven specialty HIV care clinics. We obtained chlamydia and gonorrh...
  11. Correlates of illness severity in infectious mononucleosis.

    The Canadian journal of infectious diseases = J... 25(5):277 (2014) PMID 25371691 PMCID PMC4211352

    Understanding the spectrum and frequencies of Epstein-Barr virus (EBV) complications and markers of illness severity in immunocompetent patients with primary EBV infection will inform management of patients with EBV-related illnesses. To determine the clinical and laboratory correlates of illnes...
  12. Detection and differentiation of herpes simplex viruses by use of the viper platform: advantages, limitations, and concerns.

    Journal of Clinical Microbiology 52(6):2186 (2014) PMID 24696023 PMCID PMC4042773

    The Viper HSV-Q(x) assay was evaluated for the detection of herpes simplex virus 1 (HSV-1) and HSV-2 in specimens from oral, anogenital, and other miscellaneous sites. The HSV-Q(x) assay was found to be highly sensitive and accurate; however, a gray zone may be required for specimens with values...
  13. Characterization of methicillin-resistant Staphylococcus aureus isolates from patients with persistent or recurrent bacteremia.

    The Canadian journal of infectious diseases = J... 25(2):83 (2014) PMID 24855475 PMCID PMC4028673

    Methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infections (BSI) are associated with considerable morbidity and mortality, especially with persistent (PB) or recurrent bacteremia (RB). To determine the frequency of PB and RB in patients with MRSA BSI, and to characterize the isola...
  14. Respiratory syncytial virus infection-associated hospitalization in adults: a retrospective cohort study.

    BMC Infectious Diseases 14(1):665 (2014) PMID 25494918 PMCID PMC4269936

    Once considered primarily a pediatric concern, respiratory syncytial virus (RSV) infection is gaining recognition as a cause of significant morbidity and mortality in adults. A better understanding of RSV epidemiology and disease in adults is needed to guide patient management and to assess the ...
  15. DO IT Trial: vitamin D Outcomes and Interventions in Toddlers - a TARGet Kids! randomized controlled trial.

    BMC Pediatrics 14:37 (2014) PMID 24506910 PMCID PMC3942179

    Vitamin D levels are alarmingly low (<75 nmol/L) in 65-70% of North American children older than 1 year. An increased risk of viral upper respiratory tract infections (URTI), asthma-related hospitalizations and use of anti-inflammatory medication have all been linked with low vitamin D. No study...
  16. Detection of influenza H7N9 virus: all molecular tests are not equal.

    Journal of Clinical Microbiology 51(11):3835 (2013) PMID 23966485 PMCID PMC3889775

    The recent emergence of influenza A virus (H7N9) emphasizes the need for its rapid detection. While commercial nucleic acid amplification tests (NAATs) are commonly used to detect seasonal influenza virus, this study demonstrated that the analytical sensitivity of commercial assays is highly var...
  17. Clinical characteristics and outcome associated with pandemic (2009) H1N1 influenza infection in patients with hematologic malignancies: a retrospective cohort study.

    Leukemia & Lymphoma 54(6):1250 (2013) PMID 23072372

    Pandemic H1N1 (pH1N1) influenza has been associated with a worldwide outbreak of febrile respiratory illness. Although impaired immunity, such as that caused by hematologic malignancy, has been identified as a risk factor for severe infection with this virus, the course of this infection has not...
  18. Impact of antiretroviral therapy duration and intensification on isolated shedding of HIV-1 RNA in semen.

    Journal of Infectious Diseases 207(8):1226 (2013) PMID 23329849

    Effective antiretroviral therapy (ART) dramatically reduces human immunodeficiency virus (HIV) transmission. However, isolated shedding of HIV type 1 (HIV-1) in semen (IHS) can occur in the absence of detectable viremia or genital infections. We hypothesized that ART intensification with medicat...
  19. Risk factors for influenza among health care workers during 2009 pandemic, Toronto, Ontario, Canada.

    Emerging Infectious Diseases 19(4):606 (2013) PMID 23631831 PMCID PMC3647716

    This prospective cohort study, performed during the 2009 influenza A(H1N1) pandemic, was aimed to determine whether adults working in acute care hospitals were at higher risk than other working adults for influenza and to assess risk factors for influenza among health care workers (HCWs). We ass...
  20. Performance of the xTAG® gastrointestinal pathogen panel, a multiplex molecular assay for simultaneous detection of bacterial, viral, and parasitic causes of infectious gastroenteritis.

    Journal of Microbiology and Biotechnology 23(7):1041 (2013) PMID 23711521

    The xTAG(®) Gastrointestinal Pathogen Panel (GPP) is a multiplexed molecular test for 15 gastrointestinal pathogens. The sensitivity and specificity of this test were assessed in 901 stool specimens collected from pediatric and adult patients at four clinical sites. A combination of conventional...